RX_DROME
ID RX_DROME Reviewed; 873 AA.
AC Q9W2Q1; O46035;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2001, sequence version 2.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Retinal homeobox protein Rx;
DE Short=DRx;
DE Short=DRx1;
GN Name=Rx; ORFNames=CG10052;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Canton-S; TISSUE=Embryo;
RX PubMed=9482887; DOI=10.1073/pnas.95.5.2343;
RA Eggert T., Hauck B., Hildebrandt N., Gehring W.J., Walldorf U.;
RT "Isolation of a Drosophila homolog of the vertebrate homeobox gene Rx and
RT its possible role in brain and eye development.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:2343-2348(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP PARTIAL NUCLEOTIDE SEQUENCE.
RX PubMed=9177348; DOI=10.1038/42475;
RA Mathers P.H., Grinberg A., Mahon K.A., Jamrich M.;
RT "The Rx homeobox gene is essential for vertebrate eye development.";
RL Nature 387:603-607(1997).
CC -!- FUNCTION: Appears to function in brain development.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000255|PROSITE-ProRule:PRU00138}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the procephalic region and in the
CC clypeolabrum from stage 8 on and later in the brain and the central
CC nervous system.
CC -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA11241.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AJ223300; CAA11241.1; ALT_INIT; mRNA.
DR EMBL; AE013599; AAF46639.3; -; Genomic_DNA.
DR EMBL; AY118390; AAM48419.1; -; mRNA.
DR RefSeq; NP_726006.3; NM_166413.3.
DR AlphaFoldDB; Q9W2Q1; -.
DR BioGRID; 63010; 10.
DR IntAct; Q9W2Q1; 7.
DR STRING; 7227.FBpp0071447; -.
DR PaxDb; Q9W2Q1; -.
DR PRIDE; Q9W2Q1; -.
DR DNASU; 37367; -.
DR EnsemblMetazoa; FBtr0342783; FBpp0309625; FBgn0020617.
DR GeneID; 37367; -.
DR KEGG; dme:Dmel_CG10052; -.
DR CTD; 37367; -.
DR FlyBase; FBgn0020617; Rx.
DR VEuPathDB; VectorBase:FBgn0020617; -.
DR eggNOG; KOG0490; Eukaryota.
DR HOGENOM; CLU_320970_0_0_1; -.
DR InParanoid; Q9W2Q1; -.
DR OrthoDB; 1478355at2759; -.
DR PhylomeDB; Q9W2Q1; -.
DR BioGRID-ORCS; 37367; 0 hits in 3 CRISPR screens.
DR ChiTaRS; pre-mod(mdg4)-X; fly.
DR GenomeRNAi; 37367; -.
DR PRO; PR:Q9W2Q1; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0020617; Expressed in embryonic procephalic segment and 25 other tissues.
DR Genevisible; Q9W2Q1; DM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR003654; OAR_dom.
DR InterPro; IPR032967; Rx-like.
DR PANTHER; PTHR24329:SF405; PTHR24329:SF405; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03826; OAR; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50803; OAR; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..873
FT /note="Retinal homeobox protein Rx"
FT /id="PRO_0000049288"
FT DNA_BIND 528..587
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 127..157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 183..240
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 278..339
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 374..528
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 662..810
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 822..850
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 116..123
FT /note="Octapeptide motif"
FT MOTIF 849..862
FT /note="OAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT MOTIF 855..859
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 130..144
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 294..322
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 434..477
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 484..509
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 510..527
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 676..696
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 737..810
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 4
FT /note="S -> P (in Ref. 1; CAA11241)"
FT /evidence="ECO:0000305"
FT CONFLICT 136
FT /note="R -> W (in Ref. 1; CAA11241)"
FT /evidence="ECO:0000305"
FT CONFLICT 384
FT /note="S -> T (in Ref. 1; CAA11241)"
FT /evidence="ECO:0000305"
FT CONFLICT 645..672
FT /note="PLSLAPGNLTMSSLAAMGHHHAHNGPPP -> QPGARKSDHEQSGGHGPPPC
FT PQWAAA (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 768
FT /note="G -> L (in Ref. 1; CAA11241)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 873 AA; 92897 MW; 87B56AD4693F6710 CRC64;
MSGSGGVANS SSTAAANNTA ATFQHIFEQL VQQGGGNHKL PPKQLEQLRH LLGNVRDAKN
LQMIVEKFKN LEQFHEHYAA HLANNNTVIS TEDSNDLVKD NARKYGSGGQ TLTPRHTIDA
ILGLKNRNGA ANGSGRNPET VSDGSVDPSL GDDDATDLRC GMTLTQLRSM DNHMASMLQQ
HAKNGGALPY GPPTPPGGQQ PQVPNATPLH HGQQMGGQAG HATHAGHGHP THHGHAPFGY
HNAFGFGQGG HGYGHPEEAA GNYLNSMHQM VEANQLQTGA NGANPPPAPL PPSSFGSHQQ
HLAALAAQAQ EQQNQHSKYA KSSPTGAGPP PPPGAYFMES QTAPVAPSQI NYDERSMSSA
SDLEEDDDDA AKLQLDVTSP PTPSPRGQLA AKRKSAGVFC DDNEPKLANG QLPGGNYGIR
PRSMEEVHHQ QQSHHHQQQQ QQQQQQQQLQ QQQGFQHDFR NSGNGNPNGN SNSGDHGERL
NADSDSLVNG SCASSEDLNQ TNSSEQGEKI TSGSDDEGQD DNCAKKKHRR NRTTFTTYQL
HELERAFEKS HYPDVYSREE LAMKVNLPEV RVQVWFQNRR AKWRRQEKSE SLRLGLTHFT
QLPHRLGCGA SGLPVDPWLS PPLLSALPGF LSHPQTVYPS YLTPPLSLAP GNLTMSSLAA
MGHHHAHNGP PPPHVGHGGH GQPQPPPPPP PHGVPHPHGS HHVVPLSHLS PHLSRMSPHA
TSLGSPHHGV TPLGTPLHSS LPPSSTATTV AVSSSQSSSS SASLECSGPD VCMSPQNLSI
GNADSNGDGR DLSSDLDAGS TSSNPGSSLD KCAASANIEL LDVGRDSPPP PPTPTGKGSS
TPPTDMRSNS IATLRIKAKE HLDNLNKGMV SIV
