RX_MOUSE
ID RX_MOUSE Reviewed; 342 AA.
AC O35602; O08748; Q6GT92;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Retinal homeobox protein Rx;
DE AltName: Full=Retina and anterior neural fold homeobox protein;
GN Name=Rax; Synonyms=Rx;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Embryo;
RX PubMed=9177348; DOI=10.1038/42475;
RA Mathers P.H., Grinberg A., Mahon K.A., Jamrich M.;
RT "The Rx homeobox gene is essential for vertebrate eye development.";
RL Nature 387:603-607(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9096350; DOI=10.1073/pnas.94.7.3088;
RA Furukawa T., Kozak C.A., Cepko C.L.;
RT "Rax, a novel paired-type homeobox gene, shows expression in the anterior
RT neural fold and developing retina.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:3088-3093(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Plays a critical role in eye formation by regulating the
CC initial specification of retinal cells and/or their subsequent
CC proliferation. Binds to the photoreceptor conserved element-I (PCE-
CC 1/Ret 1) in the photoreceptor cell-specific arrestin promoter (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Expressed in the photoreceptor and inner nuclear
CC layers.
CC -!- DEVELOPMENTAL STAGE: Strongly expressed in anterior neural plate at 8.5
CC dpc, followed in optic sulci and ventral forebrain at 9.0 dpc, and in
CC eye at 10.5 dpc uniformly expressed in neuroretina at 15.5 dpc, and at
CC later stages, expression decreases.
CC -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC {ECO:0000305}.
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DR EMBL; AF001906; AAB62324.1; -; mRNA.
DR EMBL; U73177; AAC53129.1; -; mRNA.
DR EMBL; CH466528; EDL09676.1; -; Genomic_DNA.
DR EMBL; BC024731; AAH24731.1; -; mRNA.
DR EMBL; BC058757; AAH58757.1; -; mRNA.
DR CCDS; CCDS29311.1; -.
DR RefSeq; NP_038861.2; NM_013833.2.
DR AlphaFoldDB; O35602; -.
DR SMR; O35602; -.
DR BioGRID; 202604; 1.
DR IntAct; O35602; 2.
DR STRING; 10090.ENSMUSP00000025396; -.
DR PhosphoSitePlus; O35602; -.
DR MaxQB; O35602; -.
DR PaxDb; O35602; -.
DR PRIDE; O35602; -.
DR ProteomicsDB; 256852; -.
DR Antibodypedia; 9851; 285 antibodies from 23 providers.
DR DNASU; 19434; -.
DR Ensembl; ENSMUST00000025396; ENSMUSP00000025396; ENSMUSG00000024518.
DR GeneID; 19434; -.
DR KEGG; mmu:19434; -.
DR UCSC; uc008ffl.1; mouse.
DR CTD; 30062; -.
DR MGI; MGI:109632; Rax.
DR VEuPathDB; HostDB:ENSMUSG00000024518; -.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000162144; -.
DR HOGENOM; CLU_047013_2_0_1; -.
DR InParanoid; O35602; -.
DR OMA; PSYPCGP; -.
DR OrthoDB; 1085093at2759; -.
DR PhylomeDB; O35602; -.
DR TreeFam; TF315976; -.
DR BioGRID-ORCS; 19434; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Rax; mouse.
DR PRO; PR:O35602; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; O35602; protein.
DR Bgee; ENSMUSG00000024518; Expressed in optic vesicle and 55 other tissues.
DR ExpressionAtlas; O35602; baseline and differential.
DR Genevisible; O35602; MM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0007420; P:brain development; IMP:MGI.
DR GO; GO:0043010; P:camera-type eye development; IMP:MGI.
DR GO; GO:0021854; P:hypothalamus development; IMP:MGI.
DR GO; GO:0060173; P:limb development; IMP:MGI.
DR GO; GO:0007389; P:pattern specification process; IMP:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR003654; OAR_dom.
DR InterPro; IPR043562; RAX/RAX2.
DR PANTHER; PTHR46271; PTHR46271; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03826; OAR; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50803; OAR; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..342
FT /note="Retinal homeobox protein Rx"
FT /id="PRO_0000049277"
FT DNA_BIND 136..195
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 44..140
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 208..295
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 33..40
FT /note="Octapeptide motif"
FT MOTIF 319..332
FT /note="OAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT MOTIF 325..329
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 48..65
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 270..295
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 12
FT /note="D -> H (in Ref. 1; AAB62324)"
FT /evidence="ECO:0000305"
FT CONFLICT 213
FT /note="S -> I (in Ref. 1; AAB62324)"
FT /evidence="ECO:0000305"
FT CONFLICT 248
FT /note="P -> T (in Ref. 1; AAB62324)"
FT /evidence="ECO:0000305"
FT CONFLICT 314..315
FT /note="AY -> RS (in Ref. 1; AAB62324)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 342 AA; 36335 MW; 8315DAF6B038D087 CRC64;
MHLPGCAPAM ADGSFSLAGH LLRSPGGSTS RLHSIEAILG FTKEDGILDT FPAERSSRSS
KERDPRLGAQ PACPKAPAGG SESSPPAAPG FVPEYEATRP CYPKEQGEAR PSPGLSVGPA
AGDSKLSEEE PPKKKHRRNR TTFTTYQLHE LERAFEKSHY PDVYSREELA GKVNLPEVRV
QVWFQNRRAK WRRQEKLEVS SMKLQDSPLL SFSRSPPSSA LAPLGTGPGS GSGPPGSALP
LEPWLGPPLP GGGATALQSL PGFGPPGQGL PASYTPPPPF LNSAPLGPGL QQLGPPPAYP
CAPAFGDKFS LEEAYPRNSS IAALRLKAKE HIQAIGKPWQ AL
