RYA_DROME
ID RYA_DROME Reviewed; 109 AA.
AC G5CKU5; A8Y508; V9I0I4;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 55.
DE RecName: Full=RYamide neuropeptides {ECO:0000303|PubMed:21704020, ECO:0000303|PubMed:21843505};
DE AltName: Full=Neuropeptide Y-like receptor ligand {ECO:0000303|PubMed:21704020, ECO:0000303|PubMed:21843505};
DE Contains:
DE RecName: Full=RYamide-1 {ECO:0000303|PubMed:21704020, ECO:0000303|PubMed:21843505};
DE Contains:
DE RecName: Full=RYamide-2 {ECO:0000303|PubMed:21704020, ECO:0000303|PubMed:21843505};
DE Flags: Precursor;
GN Name=RYa {ECO:0000303|PubMed:21704020, ECO:0000303|PubMed:21843505};
GN Synonyms=NepYr {ECO:0000303|PubMed:21704020};
GN ORFNames=CG40733 {ECO:0000312|FlyBase:FBgn0085512};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|EMBL:AEP22449.1}
RP NUCLEOTIDE SEQUENCE [MRNA], SYNTHESIS OF RYAMIDE-1 AND RYAMIDE-2, FUNCTION,
RP SUBCELLULAR LOCATION, AND AMIDATION AT TYR-33 AND TYR-63.
RX PubMed=21843505; DOI=10.1016/j.bbrc.2011.07.131;
RA Collin C., Hauser F., Krogh-Meyer P., Hansen K.K., Gonzalez de Valdivia E.,
RA Williamson M., Grimmelikhuijzen C.J.;
RT "Identification of the Drosophila and Tribolium receptors for the recently
RT discovered insect RYamide neuropeptides.";
RL Biochem. Biophys. Res. Commun. 412:578-583(2011).
RN [2] {ECO:0000312|EMBL:BAM66571.1}
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 25-33 AND 54-63, SYNTHESIS
RP OF RYAMIDE-1 AND RYAMIDE-2, FUNCTION, SUBCELLULAR LOCATION, AND AMIDATION
RP AT TYR-33 AND TYR-63.
RX PubMed=21704020; DOI=10.1016/j.bbrc.2011.06.081;
RA Ida T., Takahashi T., Tominaga H., Sato T., Kume K., Ozaki M.,
RA Hiraguchi T., Maeda T., Shiotani H., Terajima S., Sano H., Mori K.,
RA Yoshida M., Miyazato M., Kato J., Murakami N., Kangawa K., Kojima M.;
RT "Identification of the novel bioactive peptides dRYamide-1 and dRYamide-2,
RT ligands for a neuropeptide Y-like receptor in Drosophila.";
RL Biochem. Biophys. Res. Commun. 410:872-877(2011).
RN [3] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Neuropeptides RYamide-1 and RYamide-2 are ligands for the G-
CC protein coupled receptor RYa-R (PubMed:21843505, PubMed:21704020). May
CC suppress feeding behavior (PubMed:21704020).
CC {ECO:0000269|PubMed:21704020, ECO:0000269|PubMed:21843505}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:21704020,
CC ECO:0000305|PubMed:21843505}.
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DR EMBL; HQ698845; ADZ15313.1; -; mRNA.
DR EMBL; JN222358; AEP22449.1; -; mRNA.
DR EMBL; AB638268; BAM66571.1; -; mRNA.
DR EMBL; AE013599; EDP28140.3; -; Genomic_DNA.
DR RefSeq; NP_001104382.3; NM_001110912.3.
DR AlphaFoldDB; G5CKU5; -.
DR STRING; 7227.FBpp0302660; -.
DR PaxDb; G5CKU5; -.
DR EnsemblMetazoa; FBtr0310523; FBpp0302660; FBgn0085512.
DR GeneID; 5740597; -.
DR KEGG; dme:Dmel_CG40733; -.
DR CTD; 5740597; -.
DR FlyBase; FBgn0085512; RYa.
DR VEuPathDB; VectorBase:FBgn0085512; -.
DR HOGENOM; CLU_2186633_0_0_1; -.
DR OrthoDB; 1590016at2759; -.
DR ChiTaRS; RYa-R; fly.
DR GenomeRNAi; 5740597; -.
DR PRO; PR:G5CKU5; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0085512; Expressed in midgut and 11 other tissues.
DR GO; GO:0005576; C:extracellular region; IC:FlyBase.
DR GO; GO:0005615; C:extracellular space; IC:FlyBase.
DR GO; GO:0048018; F:receptor ligand activity; IPI:FlyBase.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:FlyBase.
DR GO; GO:2000252; P:negative regulation of feeding behavior; IDA:FlyBase.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Neuropeptide; Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000305|PubMed:21704020"
FT PEPTIDE 25..33
FT /note="RYamide-1"
FT /evidence="ECO:0000269|PubMed:21704020,
FT ECO:0000305|PubMed:21843505"
FT /id="PRO_0000439148"
FT PROPEP 36..53
FT /evidence="ECO:0000305"
FT /id="PRO_0000439149"
FT PEPTIDE 54..63
FT /note="RYamide-2"
FT /evidence="ECO:0000269|PubMed:21704020,
FT ECO:0000305|PubMed:21843505"
FT /id="PRO_0000439150"
FT PROPEP 67..109
FT /evidence="ECO:0000305"
FT /id="PRO_0000439151"
FT MOD_RES 33
FT /note="Tyrosine amide"
FT /evidence="ECO:0000305|PubMed:21704020,
FT ECO:0000305|PubMed:21843505"
FT MOD_RES 63
FT /note="Tyrosine amide"
FT /evidence="ECO:0000305|PubMed:21704020,
FT ECO:0000305|PubMed:21843505"
SQ SEQUENCE 109 AA; 13167 MW; 5C22387ECBEB53D4 CRC64;
MNECVNKLLH LKFLFYFILG IQKRPVFFVA SRYGRSTTYD ESLKSRRIFI VPRNEHFFLG
SRYGKRSGKY LCLSREINKL IVRKRLRNND KERTPTLSFI TKHFLMRNT
