ID   BEDB_PSEPU              Reviewed;         107 AA.
AC   Q07947;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Benzene 1,2-dioxygenase system ferredoxin subunit;
GN   Name=bedB;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OG   Plasmid pHMT112.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ML2;
RX   PubMed=8344526; DOI=10.1016/0378-1119(93)90343-2;
RA   Tan H.-M., Tang H.-Y., Joannou C., Abdel-Wahab N.H., Mason J.R.;
RT   "The Pseudomonas putida ML2 plasmid-encoded genes for benzene dioxygenase
RT   are unusual in codon usage and low in G+C content.";
RL   Gene 130:33-39(1993).
CC   -!- FUNCTION: This protein seems to be a 2Fe-2S ferredoxin.
CC   -!- PATHWAY: Aromatic compound metabolism; benzene degradation; catechol
CC       from benzene: step 1/2.
CC   -!- SUBUNIT: This dioxygenase system consists of four proteins: the two
CC       subunits of the hydroxylase component (BedC1 and BedC2), a ferredoxin
CC       (BedB) and a ferredoxin reductase (BedA).
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase
CC       ferredoxin component family. {ECO:0000305}.
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DR   EMBL; AF148496; AAA17760.1; -; Genomic_DNA.
DR   PIR; JN0811; JN0811.
DR   AlphaFoldDB; Q07947; -.
DR   SMR; Q07947; -.
DR   BioCyc; MetaCyc:MON-12883; -.
DR   UniPathway; UPA00272; UER00391.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.102.10.10; -; 1.
DR   InterPro; IPR017941; Rieske_2Fe-2S.
DR   InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR   Pfam; PF00355; Rieske; 1.
DR   SUPFAM; SSF50022; SSF50022; 1.
DR   PROSITE; PS51296; RIESKE; 1.
PE   3: Inferred from homology;
KW   2Fe-2S; Aromatic hydrocarbons catabolism; Electron transport; Iron;
KW   Iron-sulfur; Metal-binding; Plasmid; Transport.
FT   CHAIN           1..107
FT                   /note="Benzene 1,2-dioxygenase system ferredoxin subunit"
FT                   /id="PRO_0000201684"
FT   DOMAIN          4..99
FT                   /note="Rieske"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         43
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         45
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         62
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         65
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
SQ   SEQUENCE   107 AA;  11940 MW;  904BA5421B25892B CRC64;
     MTWTYILRQS DLPPGEMQRY EGGSEPVMVC NVDGEFFAVQ DTCTHGDWAL SEGYLDGDVV
     ECTLHFGKFC VRTGKVKALP ACKPIKVYPI KIEGDEVHVD LDNGELK