ID   BEDC2_PSEPU             Reviewed;         187 AA.
AC   Q07945;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Benzene 1,2-dioxygenase subunit beta;
DE            EC=1.14.12.3;
GN   Name=bedC2;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OG   Plasmid pHMT112.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-15.
RC   STRAIN=ML2;
RX   PubMed=8344526; DOI=10.1016/0378-1119(93)90343-2;
RA   Tan H.-M., Tang H.-Y., Joannou C., Abdel-Wahab N.H., Mason J.R.;
RT   "The Pseudomonas putida ML2 plasmid-encoded genes for benzene dioxygenase
RT   are unusual in codon usage and low in G+C content.";
RL   Gene 130:33-39(1993).
CC   -!- FUNCTION: The beta subunit may be responsible for the substrate
CC       specificity of the enzyme.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=benzene + H(+) + NADH + O2 = cis-1,2-dihydrobenzene-1,2-diol +
CC         NAD(+); Xref=Rhea:RHEA:13813, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16190, ChEBI:CHEBI:16716, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.14.12.3;
CC   -!- PATHWAY: Aromatic compound metabolism; benzene degradation; catechol
CC       from benzene: step 1/2.
CC   -!- SUBUNIT: This dioxygenase system consists of four proteins: the two
CC       subunits of the hydroxylase component (BedC1 and BedC2), a ferredoxin
CC       (BedB) and a ferredoxin reductase (BedA).
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase
CC       beta subunit family. {ECO:0000305}.
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DR   EMBL; AF148496; AAA17759.1; -; Genomic_DNA.
DR   PIR; JN0813; JN0813.
DR   AlphaFoldDB; Q07945; -.
DR   SMR; Q07945; -.
DR   PRIDE; Q07945; -.
DR   BioCyc; MetaCyc:MON-12881; -.
DR   UniPathway; UPA00272; UER00391.
DR   GO; GO:0018619; F:benzene 1,2-dioxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0071704; P:organic substance metabolic process; IEA:UniProt.
DR   CDD; cd00667; ring_hydroxylating_dioxygenases_beta; 1.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR000391; Rng_hydr_dOase-bsu.
DR   PANTHER; PTHR41534; PTHR41534; 1.
DR   Pfam; PF00866; Ring_hydroxyl_B; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Dioxygenase; Direct protein sequencing;
KW   NAD; Oxidoreductase; Plasmid.
FT   CHAIN           1..187
FT                   /note="Benzene 1,2-dioxygenase subunit beta"
FT                   /id="PRO_0000085066"
SQ   SEQUENCE   187 AA;  22253 MW;  ECF709BE6DF2B2BD CRC64;
     MIDSVNRADL FLRKPAPVAL ELQNEIEQFY YWEAKLLNDR RFDEWFALLA KDIHYFMPIR
     TTRIMRDSRL EYSGLRDYAH FDDDATMMKG RLRKITSDVS WSENPASRTR HIVSNVMIIP
     TEVEGEYEIS STFIVYRNRL ERQLDIFAGE RRDRLRRNKG EAGFEIVNRT ILIDQSTILA
     NNLSFFF