RYR_DROME
ID RYR_DROME Reviewed; 5127 AA.
AC Q24498; Q24321; Q24499; Q24500; Q24501; Q8MKS3; Q8MKS4; Q8MKS5; Q9V4Y7;
DT 19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 3.
DT 03-AUG-2022, entry version 183.
DE RecName: Full=Ryanodine receptor;
DE AltName: Full=Ryanodine receptor 44F;
GN Name=RyR {ECO:0000312|FlyBase:FBgn0011286};
GN Synonyms=dry, Rya-r44F {ECO:0000312|FlyBase:FBgn0011286};
GN ORFNames=CG10844 {ECO:0000312|FlyBase:FBgn0011286};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAF59036.2};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS A; B; C AND D), FUNCTION,
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=8276118; DOI=10.1016/0014-5793(94)80634-9;
RA Takeshima H., Nishi M., Iwabe N., Miyata T., Hosoya T., Masai I., Hotta Y.;
RT "Isolation and characterization of a gene for a ryanodine receptor/calcium
RT release channel in Drosophila melanogaster.";
RL FEBS Lett. 337:81-87(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305}
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley {ECO:0000269|PubMed:12537572};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE OF 4619-5127, DEVELOPMENTAL STAGE, AND TISSUE
RP SPECIFICITY.
RC TISSUE=Brain {ECO:0000269|PubMed:1338312};
RX PubMed=1338312; DOI=10.1242/dev.116.4.967;
RA Hasan G., Rosbash M.;
RT "Drosophila homologs of two mammalian intracellular Ca(2+)-release
RT channels: identification and expression patterns of the inositol 1,4,5-
RT triphosphate and the ryanodine receptor genes.";
RL Development 116:967-975(1992).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=10811919; DOI=10.1073/pnas.110145997;
RA Sullivan K.M.C., Scott K., Zuker C.S., Rubin G.M.;
RT "The ryanodine receptor is essential for larval development in Drosophila
RT melanogaster.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:5942-5947(2000).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1459, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Intracellular calcium channel that is required for proper
CC muscle function during embryonic development and may be essential for
CC excitation-contraction coupling in larval body wall muscles.
CC {ECO:0000269|PubMed:10811919, ECO:0000269|PubMed:8276118}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Sarcoplasmic reticulum membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}. Membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}. Note=The number of predicted
CC transmembrane domains varies between orthologs, but both N-terminus and
CC C-terminus seem to be cytoplasmic. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=A {ECO:0000269|PubMed:8276118};
CC IsoId=Q24498-2; Sequence=Displayed;
CC Name=B {ECO:0000269|PubMed:8276118};
CC IsoId=Q24498-3; Sequence=VSP_050196;
CC Name=C {ECO:0000269|PubMed:8276118};
CC IsoId=Q24498-4; Sequence=VSP_050195;
CC Name=D {ECO:0000269|PubMed:8276118};
CC IsoId=Q24498-5; Sequence=VSP_050195, VSP_050196;
CC -!- TISSUE SPECIFICITY: During embryonic stages 9-10, expression is seen in
CC mesoderm of all segments in progenitors of the cephalic and somatic
CC muscles. Adults exhibit high expression in tubular 'jump' muscles of
CC thorax and leg, and lower expression in the brain, ventral ganglion,
CC head muscles and proboscis muscles. {ECO:0000269|PubMed:10811919,
CC ECO:0000269|PubMed:1338312, ECO:0000269|PubMed:8276118}.
CC -!- DEVELOPMENTAL STAGE: Abundant in 6-12 hours embryos, reduced expression
CC in second and third instar larval stages and adults.
CC {ECO:0000269|PubMed:1338312, ECO:0000269|PubMed:8276118}.
CC -!- DOMAIN: The calcium release channel activity resides in the C-terminal
CC region while the remaining part of the protein resides in the
CC cytoplasm. {ECO:0000305}.
CC -!- MISCELLANEOUS: Channel activity is modulated by the alkaloid ryanodine
CC that binds to the open Ca-release channel with high affinity and
CC maintains the channel in an open conformation. The calcium release
CC channel is modulated by calcium ions, magnesium ions, ATP and
CC calmodulin (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ryanodine receptor (TC 1.A.3.1) family.
CC {ECO:0000305}.
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DR EMBL; D17389; BAA04212.1; -; Genomic_DNA.
DR EMBL; D17389; BAA41469.1; -; Genomic_DNA.
DR EMBL; D17389; BAA41470.1; -; Genomic_DNA.
DR EMBL; D17389; BAA41471.1; -; Genomic_DNA.
DR EMBL; AE013599; AAF59036.2; -; Genomic_DNA.
DR EMBL; AE013599; AAM71082.1; -; Genomic_DNA.
DR EMBL; AE013599; AAM71083.1; -; Genomic_DNA.
DR EMBL; AE013599; AAM71084.1; -; Genomic_DNA.
DR EMBL; Z18536; CAA79221.1; -; mRNA.
DR PIR; B49131; B49131.
DR PIR; S40450; S40450.
DR RefSeq; NP_476991.1; NM_057643.4. [Q24498-2]
DR RefSeq; NP_476992.1; NM_057644.4. [Q24498-3]
DR RefSeq; NP_476993.1; NM_057645.3. [Q24498-4]
DR RefSeq; NP_476994.1; NM_057646.4. [Q24498-5]
DR SMR; Q24498; -.
DR BioGRID; 71972; 11.
DR IntAct; Q24498; 13.
DR STRING; 7227.FBpp0293114; -.
DR BindingDB; Q24498; -.
DR ChEMBL; CHEMBL6045; -.
DR TCDB; 1.A.3.1.5; the ryanodine-inositol 1,4,5-triphosphate receptor ca(2+) channel (rir-cac) family.
DR iPTMnet; Q24498; -.
DR PaxDb; Q24498; -.
DR PRIDE; Q24498; -.
DR EnsemblMetazoa; FBtr0088637; FBpp0087718; FBgn0011286. [Q24498-4]
DR EnsemblMetazoa; FBtr0088638; FBpp0087719; FBgn0011286. [Q24498-3]
DR EnsemblMetazoa; FBtr0088639; FBpp0087720; FBgn0011286. [Q24498-5]
DR EnsemblMetazoa; FBtr0088640; FBpp0087721; FBgn0011286. [Q24498-2]
DR GeneID; 49090; -.
DR KEGG; dme:Dmel_CG10844; -.
DR CTD; 49090; -.
DR FlyBase; FBgn0011286; RyR.
DR VEuPathDB; VectorBase:FBgn0011286; -.
DR eggNOG; KOG2243; Eukaryota.
DR GeneTree; ENSGT00940000168850; -.
DR InParanoid; Q24498; -.
DR PhylomeDB; Q24498; -.
DR SignaLink; Q24498; -.
DR BioGRID-ORCS; 49090; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 49090; -.
DR PRO; PR:Q24498; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0011286; Expressed in oviduct (Drosophila) and 22 other tissues.
DR ExpressionAtlas; Q24498; baseline and differential.
DR Genevisible; Q24498; DM.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0042383; C:sarcolemma; IBA:GO_Central.
DR GO; GO:0033017; C:sarcoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0005790; C:smooth endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0030018; C:Z disc; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR GO; GO:0048763; F:calcium-induced calcium release activity; IBA:GO_Central.
DR GO; GO:0005219; F:ryanodine-sensitive calcium-release channel activity; TAS:UniProtKB.
DR GO; GO:0006816; P:calcium ion transport; TAS:UniProtKB.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IEA:InterPro.
DR GO; GO:0060047; P:heart contraction; IMP:FlyBase.
DR GO; GO:0006936; P:muscle contraction; IMP:UniProtKB.
DR GO; GO:0035206; P:regulation of hemocyte proliferation; IMP:FlyBase.
DR GO; GO:0072347; P:response to anesthetic; IMP:FlyBase.
DR CDD; cd12877; SPRY1_RyR; 1.
DR CDD; cd12878; SPRY2_RyR; 1.
DR CDD; cd12879; SPRY3_RyR; 1.
DR Gene3D; 2.60.120.920; -; 3.
DR InterPro; IPR001870; B30.2/SPRY.
DR InterPro; IPR043136; B30.2/SPRY_sf.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR014821; Ins145_P3_rcpt.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR036300; MIR_dom_sf.
DR InterPro; IPR016093; MIR_motif.
DR InterPro; IPR013662; RIH_assoc-dom.
DR InterPro; IPR000699; RIH_dom.
DR InterPro; IPR013333; Ryan_recept.
DR InterPro; IPR003032; Ryanodine_rcpt.
DR InterPro; IPR009460; Ryanrecept_TM4-6.
DR InterPro; IPR035910; RyR/IP3R_RIH_dom_sf.
DR InterPro; IPR035761; SPRY1_RyR.
DR InterPro; IPR035764; SPRY2_RyR.
DR InterPro; IPR035762; SPRY3_RyR.
DR InterPro; IPR003877; SPRY_dom.
DR Pfam; PF08709; Ins145_P3_rec; 1.
DR Pfam; PF00520; Ion_trans; 1.
DR Pfam; PF02815; MIR; 1.
DR Pfam; PF08454; RIH_assoc; 1.
DR Pfam; PF06459; RR_TM4-6; 1.
DR Pfam; PF01365; RYDR_ITPR; 2.
DR Pfam; PF02026; RyR; 4.
DR Pfam; PF00622; SPRY; 3.
DR PRINTS; PR00795; RYANODINER.
DR SMART; SM00472; MIR; 4.
DR SMART; SM00449; SPRY; 3.
DR SUPFAM; SSF100909; SSF100909; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR SUPFAM; SSF49899; SSF49899; 2.
DR SUPFAM; SSF82109; SSF82109; 2.
DR PROSITE; PS50188; B302_SPRY; 3.
DR PROSITE; PS50919; MIR; 5.
PE 1: Evidence at protein level;
KW Alternative splicing; Calcium; Calcium channel; Calcium transport;
KW Developmental protein; Ion channel; Ion transport;
KW Ligand-gated ion channel; Membrane; Phosphoprotein; Receptor;
KW Reference proteome; Repeat; Sarcoplasmic reticulum; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..5127
FT /note="Ryanodine receptor"
FT /id="PRO_0000219364"
FT TOPO_DOM 1..4451
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 4452..4472
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 4651..4671
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 4683..4703
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 4742..4762
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 4885..4905
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 4924..4944
FT /note="Helical"
FT /evidence="ECO:0000255"
FT INTRAMEM 4974..4983
FT /note="Pore-forming"
FT /evidence="ECO:0000250"
FT TRANSMEM 4984..5024
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 5025..5127
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 94..148
FT /note="MIR 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00131"
FT DOMAIN 155..200
FT /note="MIR 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00131"
FT DOMAIN 210..264
FT /note="MIR 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00131"
FT DOMAIN 270..328
FT /note="MIR 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00131"
FT DOMAIN 336..394
FT /note="MIR 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00131"
FT DOMAIN 577..803
FT /note="B30.2/SPRY 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00548"
FT REPEAT 845..956
FT /note="1"
FT /evidence="ECO:0000250|UniProtKB:Q15413"
FT REPEAT 957..1075
FT /note="2"
FT /evidence="ECO:0000250|UniProtKB:Q15413"
FT DOMAIN 1032..1217
FT /note="B30.2/SPRY 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00548"
FT DOMAIN 1452..1673
FT /note="B30.2/SPRY 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00548"
FT REPEAT 2822..2945
FT /note="3"
FT /evidence="ECO:0000250|UniProtKB:Q15413"
FT REPEAT 2946..3063
FT /note="4"
FT /evidence="ECO:0000250|UniProtKB:Q15413"
FT REGION 845..3063
FT /note="4 X approximate repeats"
FT REGION 1416..1453
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1477..1511
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2286..2306
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3594..3615
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3809..3831
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3864..3894
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4543..4569
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4604..4623
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4690..4714
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1421..1453
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3809..3825
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 4162
FT /note="Important for activation by Ca(2+)"
FT /evidence="ECO:0000250"
FT MOD_RES 1459
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VAR_SEQ 1135..1165
FT /note="VTKMHAGSIEHFGVRYEAGDVIGCFIDVKEQ -> EEKVYGGVSESFGKQCG
FT PGDIVGVFLDLADH (in isoform C and isoform D)"
FT /evidence="ECO:0000303|PubMed:8276118"
FT /id="VSP_050195"
FT VAR_SEQ 1876..1889
FT /note="Missing (in isoform B and isoform D)"
FT /evidence="ECO:0000303|PubMed:8276118"
FT /id="VSP_050196"
FT CONFLICT 314..316
FT /note="CLR -> SW (in Ref. 1; BAA41469/BAA41470/BAA41471/
FT BAA04212)"
FT /evidence="ECO:0000305"
FT CONFLICT 1045..1079
FT /note="VRTLLVYGYVLDPPTGEGTEALLAEAQRLKFAGFR -> SANAPGLRICLGS
FT SDGRRNGGTSGRGTTPQVRRIP (in Ref. 1; BAA41469/BAA41470/
FT BAA41471/BAA04212)"
FT /evidence="ECO:0000305"
FT CONFLICT 2282
FT /note="L -> I (in Ref. 1; BAA41469/BAA41470/BAA41471/
FT BAA04212)"
FT /evidence="ECO:0000305"
FT CONFLICT 2550
FT /note="I -> S (in Ref. 1; BAA41469/BAA41470/BAA41471/
FT BAA04212)"
FT /evidence="ECO:0000305"
FT CONFLICT 2927
FT /note="A -> P (in Ref. 1; BAA41469/BAA41470/BAA41471/
FT BAA04212)"
FT /evidence="ECO:0000305"
FT CONFLICT 3214
FT /note="L -> Q (in Ref. 1; BAA41469/BAA41470/BAA41471/
FT BAA04212)"
FT /evidence="ECO:0000305"
FT CONFLICT 3361
FT /note="A -> G (in Ref. 1; BAA41469/BAA41470/BAA41471/
FT BAA04212)"
FT /evidence="ECO:0000305"
FT CONFLICT 4096
FT /note="T -> I (in Ref. 1; BAA41469/BAA41470/BAA41471/
FT BAA04212)"
FT /evidence="ECO:0000305"
FT CONFLICT 4633
FT /note="Y -> D (in Ref. 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 4695
FT /note="I -> T (in Ref. 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 5022..5024
FT /note="DAF -> AL (in Ref. 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 5073
FT /note="M -> I (in Ref. 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 5077
FT /note="M -> L (in Ref. 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 5103..5127
FT /note="WDFFPVGDCFRKQYEDELSGGGGGG -> GTSSQWETASASNTRMSFPAEAA
FT AA (in Ref. 4)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 5127 AA; 581012 MW; 01B637D778DECE6A CRC64;
MAEAEGGSEQ DDVSFLRTED MVTLSCTATG ERVCLAAEGF GNRHCFLENI ADKNVPPDLS
QCVFVIEQAL SVRALQELVT AAGSETGKGT GSGHRTLLYG NAILLRHHNS DMYLACLSTS
SSNDKLSFDV GLQEHSQGEA CWWTVHPASK QRSEGEKVRV GDDLILVSVA TERYLHTTKE
NEQSIVNASF HVTHWSVQPY GTGISRMKYV GYVFGGDVLR FFHGGDECLT IPSTWGREAG
QNIVIYEGGV VMAQARSLWR LELARTKWTG GFINWYHPMR IRHITTGRYL GVNDSNELIL
VKKEEASIAT TTFCLRQEKD DEKKVLEDKD LEVIGSPIIK YGDTTVIVQH CETSLWLSYK
SYETKKKGVG KVEEKQAILH EEGKMDDCLD FSRSQEEESK TARVIRKCSS LFTQFITALE
TLQSNRRHSI FFQKVNLNEM VMCLEDLINY FSQPEDDMEH EEKQNRFRAL RNRQDLFQEE
GVLNLILEAI DKINIITSQG FLASFLAGDE TGQSWDLIST YLYQLLAAII KGNHTNCAQF
ANSNRLNWLF SRLGSQASSE GSGMLDVLHC VLIDSPEALN MMRDEHIKVI ISLLEKHGRD
PKVLDVLCSL CVGNGVAVRS SQNNICDFLL PGKNLLLQTL LVDHVASIRP NIFVGRVDGS
SMYQKWYFEV TMDHIEQTTH MMPHLRIGWA NTSGYVPYPG GGKKWGGNGV GDDLYSFGFD
GAFLWTGGRK TLVVDALPEE PFIRKGDVIG VAIDLSVPII TFTFNGVKVR GSFRDFNLDG
MFFPVMSCSS KLSCRFLFGG DHGRLKFAPP MGFSALVQCL MPQQILSLDP CFYFGNLAKN
VLAGPWLIED DTAFVPKPVD TTGVTLPSSV DQIKEKLAEN IHEMWALNKI EAGWSWGEHR
DDYHRIHPCL THFEKLPAAE KRYDNQLAVQ TLKTIISLGY YITMDKPPAR IRPVRLPNEI
FMQGNGYKPA PLDLSAVTLT PKLEELVDQL AENTHNLWAR ERIQQGWTYG LNEDSENHRS
PHLVPYAKVD EAIKKANRDT ASETVRTLLV YGYVLDPPTG EGTEALLAEA QRLKFAGFRT
YRVERNYAVT SGKWYFEFEV LTSGPMRVGW ARADCYPGAM LGSEDTSWAF DGHNVTKMHA
GSIEHFGVRY EAGDVIGCFI DVKEQTISFS LNGELLMDAL GGETTFADVT AEGVGFVPAC
TLGVGQKARL IYGQDVDSLK FFTTCGLQEG YEPFCVNMRR PVTHWYTKDQ PIFENTEEMP
DCRIDVTRIP GGADTPPHLK ISHNTFETME KANWEFLRLS LPVTCMGEFI SEQEKARRWD
EIKNRQYRLM REAEIAAQMQ VQTQAAHMDH MLKGGFNMND IKGLTRNFDE HADAEADHMM
RGPNRPPRKG SLTRNITFET DMSAALDEMQ RSTSVLDMNG LGEEMDDKKK RGRSPFKFFS
KKSRDQSREK MGARTLDTSL ERRNTVAHGR NVVNQQMTTR APTLRLNNAE IPPSPVPQGP
KQLSGSNLGQ QPVETSGDEM FDAECLKLIN EYFYGVRIFP GQDPTHVYVG WVTTQYHLHS
REFNKNKVRR GSVYIEDDYE MAIERIDRQS CYVVRADELF NEVTQDASGK GASQGMFVGC
FVDTATGIIR FTCEGKDTSH RWMMEPDTKL FPAIFVEATS KEILQIELGR TPTTLPLSAA
VLPTSDKHIN PQSPPRLKVQ CLRPHQWARV PNTALQVHAL KLSDVRGWSM LCEDPVSMLA
LHIPEEDRCI DILELIEMDK LLSFHAHSLT LYAALCYQSN YRAAHALCQH VDQKQLLYAI
RSEYMSGPLR QGFYDLLIAL HLESHATTME VCKNEYITPL GAELKELYSD EEMQHSLRSL
VTESVRPQLR MTEITPPVIA TSSMPSVSSE PIPDIDQLYS PKFPLEVVRQ FVMEALKDAV
EINQVHNRDP IGWTNENLFL PLIKLTDRLL LVGVLTDEDV QRLLVMIDPE TWDQAFEREG
KDEHRKGLLT MKMAEGAKLQ MCYLLHHLYD TQLRHRVESI IAFSHDFVGD LQTDQLRRYI
EIKQSDLPSA VAAKKTKEFR CPPREQMNQI LCFKNLEPDD QDNCTCGLEL RGRLGDFHDS
LMQKVSLNAL QEPDGVEGTA IEEVKTGPIT KIYNFINTVK ELEEGPKEVE EPEKKTPEEV
FRKVLIKTIV SWAEESQIEN PKLVREMFSL LLRQYDTVGE LVRALEKTYV INTRARDDVA
EMWVGLSQIR ALLPVQMSQE EEELMRKRLW KLVNNATFFQ HPDLIRILRV HENVMAVMMN
TLGRRAQAQS DAPTQSEVAE GAPSKEKDTS HEMVVACCRF LCYFCRTGRQ NQKAMFDHFD
FLLDNANILL ARPSLRGSTP LDVAYSSLME NTELALALRE HYLEKIAVYL SRCGLQSNSE
LVEKGYPDLG WDPVEGERYL DFLRYCVWVN GESVEENANL VIRLLIRRPE CLGPALRGEG
EGLFRAIVEA NRMSERISDR CKMQDEAEGT IAGLNFTHPL PEGEEDEDYI DTGAAILNFY
CTLVDLLGRC APDASVIEQG KNESLRARAI LRSLVPLEDL QGVLSLKFTL SQTAPGEEKP
KSDMPSGLLP NNKQSIVLFL ERVYGIEAQD LFYRLLEDAF LPDLRTATIL DKSDGSESDM
ALAMNRYIGN SILPLLIKHS KFYNEAENYA SLLDATLHTV YRLSKNRMLT KGQREAVSDF
LVALTSQMQP AMLLKLLRKL TVDVSKLSEY TTVALRLLTL HFDRCAKYYG STQGQGSYGA
SSDEEKRLTM LLFSNIFDSL SNMDYDPELF GKALPCLIAI GCALPPDYSL SKNTDEDYYG
RQMGAPDQPQ YMPNPIDTNN VHLDNDLNSL VQKFSEHYHD AWASRRLEGG WTYGDIRSDN
DRKHPRLKPY NMLSEYERER YRDPVRECLK GLLAIGWTVE HSEVEVALNH RGSTRRQSKP
QINEFQNEGS PFNYNPHPVD MSNLTLSREM QNMAERLAEN SHDIWAKKKN EELNGCGGVI
HPQLVPYDLL TDKEKKKDRE RSQEFLKYMQ YQGYKLHKPS KGGAVEEGGA TQAAVELRFS
YSLLEKLIQY LDRATINMKL LKPSTTFSRR SSFKTATRDI KFFSKVVLPL MEKYFSTHRN
YFIAIATATN NIGAASLKEK EMVASIFCKL AALLRNRLSA FGPDVRITVR CLQVLVKGID
ARTLTKNCPE FIRTSMLTFF NQTSDDLGNT ILNLQDGKYS HLRGTHLKTS TSLGYVNQVV
LPVLTAMFDH LAACDYGSDL LLDEIQVASY KILAALYHLG TDGTLTHDRK YLKTEIERHR
PALGSCLGAY SSCFPVAFLE PHLNKHNQYS LLNRIADHSL EAQDIMVKME SCMPNLETIL
AEVDQFVESD KTYNDAPHII DVILPLLCAY LPFWWSQGPD NVSPTSGNHV TMVTADHMNP
LLRNVLKMIK KNIGNDNAPW MTRIAAYTQQ IIINTSEELL KDPFLPLAER VKKRTENMLH
KEDSMRGFIK SATDDTSQVE TQLQEDWNLL VRDIYSFYPL LIKYVDLQRN HWLKDNIPEA
EELYNHVAEI FNIWSKSQYF LKEEQNFISA NEIDNMALIM PTATRRSAIS EGAPAVGGKV
KKKKKNRDKK RDKDKEVQAS LMVACLKRLL PVGLNLFAGR EQELVQHCKD RYLKKMPEYD
VIEFARNQLT LPDKLDPSDE MSWQHYLYSK LGKTEEPVDE QALEKANVNS NEKGKDKTQE
TVDRIVAMAK VLFGLHMIDH PQQQSKNVYR SVVSIQRKRA VIACFRQTSL HSLPRHRACN
IFARSYYEQW LQEENVGQEV MVEDLTQTFE DSEKSKKEGE ETDSKPDPLT QLVTTFCRGA
MTERSGALQE DLLYMSYAQI AAKSTGKEEE EGGDEEGGEG GEEGEGTSIH EQEMEKQKLL
FHQARLSNRG VAEMVLLHIS ASKGIPSEMV MTTLNLGIAI LRGGNIDIQM GMLNHLKEKK
DVGFFTSIAG LMNSCSVLDL DAFERNTKAE GLGVGSEGAA GEKNMHDAEF TCALFRFIQL
TCEGHNLEWQ NYLRTQAGNT TTVNVVICTV DYLLRLQESI MDFYWHYSSK EIIDPAGKAN
FFKAIEVASQ VFNTLTEVIQ GPCTLNQQAL AHSRLWDAVG GFLFLFSHMQ DKLSKHSSQV
DLLKELLNLQ KDMITMMLSM LEGNVVNGTI GKQMVDTLVE SASNVELILK YFDMFLKLAD
LIESPSFHEV DMKNEGWVTP KDFREKMEQS KNYTPEEMDF LLACCERNHE GKIDYRAFVE
HFHEPSKEIG FNLAVLLTNL SEHMPNEPRL ARFLETAGSV LNYFEPFLGR IEILGSSKRI
ERVYFEIKDS NIEQWEKPQI RESKRAFFYS IVTEGGDKEK LEAFVNFCED AIFEMTHASG
LMATDDGGGN VKRDTAYSSY MSEEEEERAA RDPIRRTITA VKEGLKFGVH MLSPANIKHQ
IGVMQTKSIP ELIVGFFKII FYIFYYTGYA HFCVVRYIFG ILLNLMRGPA PEQEEEPVVE
EETFGRALPP LPLEEPPGTV QAFGLDINKE ENGMYKVVVH ESPANSSMEE GGESSPEDGA
AASGELVEGE PHQEPISIVD LLGGEAAKKA AQERQEAQKA QEAAMASIEA EAKKSSSAPQ
ETPAVHQIDF SQYTHRAVSF LARNFYNLKY VALVLAFSIN FMLLFYKVTS FTEEADSSAE
EELILGSGSG GGADITGSGF GGSGDGGSGD GEMEDEIPEL VHVDEDFFYM EHVLRIAACL
HSLVSLAMLI AYYHLKVPLA IFKREKEIAR RLEFEGLFIA EQPEDDDFKS HWDKLVISAK
SFPVNYWDKF VKKKVRQKYS ETYDFDSISN LLGMEKSTFA AQESEETGIF KYIMNIDWRY
QVWKAGVTFT DNAFLYSLWY FSFSVMGNFN NFFFAAHLLD VAVGFKTLRT ILQSVTHNGK
QLVLTVMLLT IIVYIYTVIA FNFFRKFYIQ EEDEEVDKKC HDMLTCFVFH LYKGVRAGGG
IGDEIGDPDG DDYEVYRIIF DITFFFFVII ILLAIIQGLI IDAFGELRDQ LESVKDNMES
NCFICGMGKD FFDIVPHGFD THVQKEHNLA NYMFFLMHLI NKPDTEYTGQ ETYVWNMYQQ
RSWDFFPVGD CFRKQYEDEL SGGGGGG
