ID   RZOD_ECOLI              Reviewed;          60 AA.
AC   P58041; Q2EEQ5; Q2MBM4;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   27-APR-2001, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Prophage outer membrane lipoprotein RzoD {ECO:0000305};
DE            Short=o-spanin;
DE   AltName: Full=Outer membrane lipoprotein Rz1 from lambdoid prophage DLP12;
DE   AltName: Full=Spanin from lambdoid prophage DLP12, outer membrane subunit;
DE   Flags: Precursor;
GN   Name=rzoD; OrderedLocusNames=b4510, JW5080;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Component of the spanin complex that disrupts the outer
CC       membrane and causes cell lysis during virus exit. The spanin complex
CC       conducts the final step in cell lysis by disrupting the outer membrane
CC       after holin and endolysin action have permeabilized the inner membrane
CC       and degraded the host peptidoglycans (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer; disulfide-linked. Interacts (via C-terminus) with
CC       RZ (via C-terminus). Part of the spanin complex which spans the entire
CC       periplasmic space. The spanin complex is composed of spanin, inner
CC       membrane subunit and spanin, outer membrane subunit (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}; Periplasmic side {ECO:0000250}.
CC   -!- MISCELLANEOUS: Encoded by the cryptic lambdoid prophage DLP12.
CC   -!- SIMILARITY: Belongs to the lambdalikevirus o-spanin family.
CC       {ECO:0000305}.
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DR   EMBL; U00096; ABD18640.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76332.1; -; Genomic_DNA.
DR   RefSeq; WP_001228697.1; NZ_CP047127.1.
DR   RefSeq; YP_588440.1; NC_000913.3.
DR   AlphaFoldDB; P58041; -.
DR   BioGRID; 4259886; 8.
DR   STRING; 511145.b4510; -.
DR   PaxDb; P58041; -.
DR   PRIDE; P58041; -.
DR   DNASU; 1450242; -.
DR   EnsemblBacteria; ABD18640; ABD18640; b4510.
DR   EnsemblBacteria; BAE76332; BAE76332; BAE76332.
DR   GeneID; 1450242; -.
DR   KEGG; ecj:JW5080; -.
DR   KEGG; eco:b4510; -.
DR   HOGENOM; CLU_146388_1_0_6; -.
DR   BioCyc; EcoCyc:MON0-2658; -.
DR   PRO; PR:P58041; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0019076; P:viral release from host cell; IEA:InterPro.
DR   InterPro; IPR010346; O-spanin.
DR   Pfam; PF06085; Rz1; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Cytolysis; Disulfide bond; Host cell lysis by virus;
KW   Lipoprotein; Membrane; Palmitate; Reference proteome; Signal;
KW   Viral release from host cell.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           20..60
FT                   /note="Prophage outer membrane lipoprotein RzoD"
FT                   /id="PRO_0000003365"
FT   LIPID           20
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           20
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   DISULFID        29
FT                   /note="Interchain"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   60 AA;  6690 MW;  30E5AF0DC674AA6C CRC64;
     MRKLKMMLCV MMLPLVVVGC TSKQSVSQCV KPPRPPAWIM QPPPDWQTPL NGIISPSERG