BEE2_ARATH
ID BEE2_ARATH Reviewed; 304 AA.
AC Q93VJ4; O23226; Q3E9Q1; Q6YJU3; Q8LF81; Q8S3E4; Q940I6;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Transcription factor BEE 2;
DE AltName: Full=Basic helix-loop-helix protein 58;
DE Short=AtbHLH58;
DE Short=bHLH 58;
DE AltName: Full=Protein Brassinosteroid enhanced expression 2;
DE AltName: Full=Transcription factor EN 80;
DE AltName: Full=bHLH transcription factor bHLH058;
GN Name=BEE2; Synonyms=BHLH58, EN80; OrderedLocusNames=At4g36540;
GN ORFNames=AP22.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INDUCTION, GENE
RP FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia; TISSUE=Stem;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 17-304 (ISOFORM 1), FUNCTION, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=12454087; DOI=10.1093/genetics/162.3.1445;
RA Friedrichsen D.M., Nemhauser J., Muramitsu T., Maloof J.N., Alonso J.,
RA Ecker J.R., Furuya M., Chory J.;
RT "Three redundant brassinosteroid early response genes encode putative bHLH
RT transcription factors required for normal growth.";
RL Genetics 162:1445-1456(2002).
RN [8]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [9]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
CC -!- FUNCTION: Positive regulator of brassinosteroid signaling.
CC {ECO:0000269|PubMed:12454087}.
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- INTERACTION:
CC Q93VJ4; A0A384L5D0: At3g29370; NbExp=3; IntAct=EBI-4424312, EBI-25520520;
CC Q93VJ4; Q9C8Z9: BHLH148; NbExp=4; IntAct=EBI-4424312, EBI-4434374;
CC Q93VJ4; Q9SKX1: IBH1; NbExp=4; IntAct=EBI-4424312, EBI-4433589;
CC Q93VJ4; Q9M0B9: IBL1; NbExp=3; IntAct=EBI-4424312, EBI-15192969;
CC Q93VJ4; Q38845: PP2AA1; NbExp=3; IntAct=EBI-4424312, EBI-1645478;
CC Q93VJ4; Q9C9L2: TCP15; NbExp=3; IntAct=EBI-4424312, EBI-4426144;
CC Q93VJ4; Q9LQF0: TCP23; NbExp=5; IntAct=EBI-4424312, EBI-15192297;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q93VJ4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q93VJ4-2; Sequence=VSP_027917;
CC -!- TISSUE SPECIFICITY: Expressed in stems and flowers.
CC {ECO:0000269|PubMed:12679534}.
CC -!- INDUCTION: Induced by flagellin, jasmonic acid (JA), brassinosteroid
CC and cytokinin, and repressed by abscisic acid. Insensitive to
CC gibberellic acid. {ECO:0000269|PubMed:12454087,
CC ECO:0000269|PubMed:12679534}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Redundant with BEE1 and
CC BEE3. {ECO:0000269|PubMed:12454087}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM61556.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAB16839.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80320.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF488591; AAM10947.1; -; mRNA.
DR EMBL; Z99708; CAB16839.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161589; CAB80320.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86669.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86670.1; -; Genomic_DNA.
DR EMBL; AF370231; AAK44046.1; -; mRNA.
DR EMBL; AY059122; AAL15228.1; -; mRNA.
DR EMBL; AY054588; AAK96779.1; -; mRNA.
DR EMBL; BT000388; AAN15707.1; -; mRNA.
DR EMBL; AY084997; AAM61556.1; ALT_INIT; mRNA.
DR EMBL; AY138254; AAN18284.1; -; mRNA.
DR PIR; D85431; D85431.
DR RefSeq; NP_195372.1; NM_119817.3. [Q93VJ4-1]
DR RefSeq; NP_849508.1; NM_179177.2. [Q93VJ4-2]
DR AlphaFoldDB; Q93VJ4; -.
DR SMR; Q93VJ4; -.
DR BioGRID; 15088; 48.
DR IntAct; Q93VJ4; 46.
DR STRING; 3702.AT4G36540.1; -.
DR PaxDb; Q93VJ4; -.
DR PRIDE; Q93VJ4; -.
DR ProteomicsDB; 240823; -. [Q93VJ4-1]
DR EnsemblPlants; AT4G36540.1; AT4G36540.1; AT4G36540. [Q93VJ4-1]
DR EnsemblPlants; AT4G36540.2; AT4G36540.2; AT4G36540. [Q93VJ4-2]
DR GeneID; 829806; -.
DR Gramene; AT4G36540.1; AT4G36540.1; AT4G36540. [Q93VJ4-1]
DR Gramene; AT4G36540.2; AT4G36540.2; AT4G36540. [Q93VJ4-2]
DR KEGG; ath:AT4G36540; -.
DR Araport; AT4G36540; -.
DR TAIR; locus:2115200; AT4G36540.
DR eggNOG; ENOG502SH3S; Eukaryota.
DR InParanoid; Q93VJ4; -.
DR OMA; WDVHSQC; -.
DR PhylomeDB; Q93VJ4; -.
DR PRO; PR:Q93VJ4; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q93VJ4; baseline and differential.
DR Genevisible; Q93VJ4; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0045824; P:negative regulation of innate immune response; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0006351; P:transcription, DNA-templated; IMP:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR031064; bHLH_BEE-like_plant.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR024097; bHLH_ZIP_TF.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR PANTHER; PTHR12565; PTHR12565; 1.
DR PANTHER; PTHR12565:SF404; PTHR12565:SF404; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..304
FT /note="Transcription factor BEE 2"
FT /id="PRO_0000302043"
FT DOMAIN 147..197
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 74..132
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 74..89
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..118
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 226..227
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_027917"
FT CONFLICT 81
FT /note="N -> Y (in Ref. 1; AAM10947)"
FT /evidence="ECO:0000305"
FT CONFLICT 297
FT /note="V -> I (in Ref. 6; AAM61556)"
FT /evidence="ECO:0000305"
FT CONFLICT 299
FT /note="N -> S (in Ref. 5; AAN15707/AAK96779)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 304 AA; 34674 MW; ACDDD85B8D575B88 CRC64;
MDLSVLDRLK WLQQQQMVSP EFLQILGSDG REELKRVESY LGNNNDELQS FRHFPEFGPD
YDTTDGCISR TSSFHMEPVK NNGHSRAITL QNKRKPEGKT EKREKKKIKA EDETEPSMKG
KSNMSNTETS SEIQKPDYIH VRARRGEATD RHSLAERARR EKISKKMKCL QDIVPGCNKV
TGKAGMLDEI INYVQSLQQQ VEFLSMKLSV INPELECHID DLSAKQFQAY FTGPPEGDSK
QSIMADFRSF PLHQQGSLDY SVINSDHTTS LGAKDHTSSS WETHSQCLYN SLRTDSVSNF
FSLK
