BEE3_ARATH
ID BEE3_ARATH Reviewed; 261 AA.
AC Q8GWK7; Q9C9S9;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Transcription factor BEE 3;
DE AltName: Full=Basic helix-loop-helix protein 50;
DE Short=AtbHLH50;
DE Short=bHLH 50;
DE AltName: Full=Protein Brassinosteroid enhanced expression 3;
DE AltName: Full=Transcription factor EN 76;
DE AltName: Full=bHLH transcription factor bHLH050;
GN Name=BEE3; Synonyms=BHLH50, EN76; OrderedLocusNames=At1g73830;
GN ORFNames=F25P22.25;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia; TISSUE=Stem;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=12454087; DOI=10.1093/genetics/162.3.1445;
RA Friedrichsen D.M., Nemhauser J., Muramitsu T., Maloof J.N., Alonso J.,
RA Ecker J.R., Furuya M., Chory J.;
RT "Three redundant brassinosteroid early response genes encode putative bHLH
RT transcription factors required for normal growth.";
RL Genetics 162:1445-1456(2002).
RN [7]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
CC -!- FUNCTION: Positive regulator of brassinosteroid signaling.
CC {ECO:0000269|PubMed:12454087}.
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8GWK7-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in stems. {ECO:0000269|PubMed:12679534}.
CC -!- INDUCTION: Induced by brassinosteroid, auxin and ethylene, and
CC repressed by abscisic acid. Insensitive to gibberellic acid.
CC {ECO:0000269|PubMed:12454087}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Redundant with BEE1 and
CC BEE2. {ECO:0000269|PubMed:12454087}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG52074.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AF488585; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF488585; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AC012679; AAG52074.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35513.1; -; Genomic_DNA.
DR EMBL; AK118781; BAC43374.1; -; mRNA.
DR EMBL; BT006055; AAP04040.1; -; mRNA.
DR RefSeq; NP_177524.2; NM_106043.3. [Q8GWK7-1]
DR AlphaFoldDB; Q8GWK7; -.
DR SMR; Q8GWK7; -.
DR BioGRID; 28938; 8.
DR IntAct; Q8GWK7; 9.
DR MINT; Q8GWK7; -.
DR STRING; 3702.AT1G73830.1; -.
DR PaxDb; Q8GWK7; -.
DR PRIDE; Q8GWK7; -.
DR EnsemblPlants; AT1G73830.1; AT1G73830.1; AT1G73830. [Q8GWK7-1]
DR GeneID; 843719; -.
DR Gramene; AT1G73830.1; AT1G73830.1; AT1G73830. [Q8GWK7-1]
DR KEGG; ath:AT1G73830; -.
DR Araport; AT1G73830; -.
DR TAIR; locus:2027809; AT1G73830.
DR eggNOG; ENOG502QTM9; Eukaryota.
DR InParanoid; Q8GWK7; -.
DR OMA; NGTKNSW; -.
DR PhylomeDB; Q8GWK7; -.
DR PRO; PR:Q8GWK7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8GWK7; baseline and differential.
DR Genevisible; Q8GWK7; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0006351; P:transcription, DNA-templated; IMP:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR031064; bHLH_BEE-like_plant.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR024097; bHLH_ZIP_TF.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR PANTHER; PTHR12565; PTHR12565; 1.
DR PANTHER; PTHR12565:SF346; PTHR12565:SF346; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..261
FT /note="Transcription factor BEE 3"
FT /id="PRO_0000302044"
FT DOMAIN 153..203
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 72..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 242..261
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 94..126
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 131..158
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 261 AA; 29499 MW; 61DBE158B7764EF6 CRC64;
MANLSSDFQT FTMDDPIRQL AELSNTLHHF QTFPPPFSSS LDSLFFHNQF PDHFPGKSLE
NNFHQGIFFP SNIQNNEESS SQFDTKKRKS LMEAVSTSEN SVSDQTLSTS SAQVSINGNI
STKNNSSRRG KRSKNREEEK EREVVHVRAR RGQATDSHSI AERVRRGKIN ERLKCLQDIV
PGCYKTMGMA TMLDEIINYV QSLQNQVEFL SMKLTAASSY YDFNSETDAV ESMQKAKARE
AVEMGQGRDG SSVFHSSSWT L
