S100_LEPPA
ID S100_LEPPA Reviewed; 75 AA.
AC P82978;
DT 16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Calhepatin;
OS Lepidosiren paradoxus (South American lungfish).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Dipnomorpha; Ceratodontiformes; Lepidosirenoidei; Lepidosirenidae;
OC Lepidosiren.
OX NCBI_TaxID=7883;
RN [1]
RP PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, ACETYLATION AT SER-1, AND
RP 3D-STRUCTURE MODELING.
RC TISSUE=Liver;
RX PubMed=12135482; DOI=10.1046/j.1432-1033.2002.03023.x;
RA Di Pietro S.M., Santome J.A.;
RT "Structural and biochemical characterization of calhepatin, an S100-like
RT calcium-binding protein from the liver of lungfish (Lepidosiren
RT paradoxa).";
RL Eur. J. Biochem. 269:3433-3441(2002).
CC -!- FUNCTION: Binds both calcium and copper, but not zinc. May be involved
CC in calcium signal transduction. {ECO:0000269|PubMed:12135482}.
CC -!- SUBUNIT: Monomer and homodimer.
CC -!- TISSUE SPECIFICITY: Liver, and to a much lower level intestine.
CC -!- MASS SPECTROMETRY: Mass=8672; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:12135482};
CC -!- SIMILARITY: Belongs to the S-100 family. {ECO:0000305}.
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DR AlphaFoldDB; P82978; -.
DR SMR; P82978; -.
DR iPTMnet; P82978; -.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW Acetylation; Calcium; Copper; Direct protein sequencing; Metal-binding;
KW Repeat.
FT CHAIN 1..75
FT /note="Calhepatin"
FT /id="PRO_0000144035"
FT DOMAIN 2..37
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 38..73
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 15
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 17
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 19
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 21
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 26
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 51
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 53
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 55
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 57
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 62
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT MOD_RES 1
FT /note="N-acetylserine"
FT /evidence="ECO:0000269|PubMed:12135482"
SQ SEQUENCE 75 AA; 8628 MW; A349C704FDD6D753 CRC64;
SADEQKLRER FEALDKDKSG TLSVDELYEG VHAVHPKVSR NDIVKIIEKV DTNKDGQVSW
QEFIEAFKRL ADLKL