S10A5_RAT
ID S10A5_RAT Reviewed; 93 AA.
AC P63083; O88945; P82540;
DT 13-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Protein S100-A5;
DE AltName: Full=Protein S-100D;
DE AltName: Full=S100 calcium-binding protein A5;
GN Name=S100a5; Synonyms=S100d;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP PROTEIN SEQUENCE, FUNCTION, SUBUNIT, AND MASS SPECTROMETRY.
RC STRAIN=Sprague-Dawley; TISSUE=Brain;
RX PubMed=10882717; DOI=10.1074/jbc.m002260200;
RA Schaefer B.W., Fritschy J.-M., Murmann P., Troxler H., Durussel I.,
RA Heizmann C.W., Cox J.A.;
RT "Brain S100A5 is a novel calcium-, zinc-, and copper ion-binding protein of
RT the EF-hand superfamily.";
RL J. Biol. Chem. 275:30623-30630(2000).
CC -!- FUNCTION: Binds calcium, zinc and copper. One subunit can
CC simultaneously bind 2 calcium ions or 2 copper ions plus 1 zinc ion.
CC Calcium and copper ions compete for the same binding sites.
CC {ECO:0000269|PubMed:10882717}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:10882717}.
CC -!- MASS SPECTROMETRY: Mass=10878; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:10882717};
CC -!- SIMILARITY: Belongs to the S-100 family. {ECO:0000305}.
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DR RefSeq; NP_001099908.1; NM_001106438.1.
DR RefSeq; XP_006232663.1; XM_006232601.3.
DR RefSeq; XP_017446250.1; XM_017590761.1.
DR RefSeq; XP_017446251.1; XM_017590762.1.
DR RefSeq; XP_017446252.1; XM_017590763.1.
DR AlphaFoldDB; P63083; -.
DR SMR; P63083; -.
DR STRING; 10116.ENSRNOP00000015712; -.
DR PaxDb; P63083; -.
DR PRIDE; P63083; -.
DR ABCD; P63083; 1 sequenced antibody.
DR Ensembl; ENSRNOT00000015712; ENSRNOP00000015712; ENSRNOG00000011748.
DR GeneID; 295211; -.
DR KEGG; rno:295211; -.
DR UCSC; RGD:1308996; rat.
DR CTD; 6276; -.
DR RGD; 1308996; S100a5.
DR eggNOG; ENOG502S40V; Eukaryota.
DR GeneTree; ENSGT00940000161986; -.
DR HOGENOM; CLU_138624_2_0_1; -.
DR InParanoid; P63083; -.
DR OMA; HAVMETP; -.
DR OrthoDB; 1563974at2759; -.
DR PhylomeDB; P63083; -.
DR TreeFam; TF332727; -.
DR PRO; PR:P63083; -.
DR Proteomes; UP000002494; Chromosome 2.
DR Bgee; ENSRNOG00000011748; Expressed in thymus and 15 other tissues.
DR Genevisible; P63083; RN.
DR GO; GO:0043025; C:neuronal cell body; ISO:RGD.
DR GO; GO:0005634; C:nucleus; ISO:RGD.
DR GO; GO:0005509; F:calcium ion binding; ISS:UniProtKB.
DR GO; GO:0048306; F:calcium-dependent protein binding; IBA:GO_Central.
DR GO; GO:0005507; F:copper ion binding; ISS:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:RGD.
DR GO; GO:0008270; F:zinc ion binding; ISS:UniProtKB.
DR CDD; cd00213; S-100; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR034325; S-100_dom.
DR InterPro; IPR028497; S100-A5.
DR InterPro; IPR001751; S100/CaBP7/8-like_CS.
DR InterPro; IPR013787; S100_Ca-bd_sub.
DR PANTHER; PTHR11639:SF65; PTHR11639:SF65; 1.
DR Pfam; PF01023; S_100; 1.
DR SMART; SM00054; EFh; 1.
DR SMART; SM01394; S_100; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
DR PROSITE; PS00303; S100_CABP; 1.
PE 1: Evidence at protein level;
KW Calcium; Copper; Direct protein sequencing; Metal-binding;
KW Reference proteome; Repeat; Zinc.
FT CHAIN 1..93
FT /note="Protein S100-A5"
FT /id="PRO_0000143982"
FT DOMAIN 12..47
FT /note="EF-hand 1"
FT /evidence="ECO:0000305"
FT DOMAIN 48..83
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 28
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /ligand_note="low affinity"
FT /evidence="ECO:0000305"
FT BINDING 33
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /ligand_note="low affinity"
FT /evidence="ECO:0000305"
FT BINDING 61
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /ligand_note="high affinity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 63
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /ligand_note="high affinity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 65
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /ligand_note="high affinity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 67
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /ligand_note="high affinity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 72
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /ligand_note="high affinity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ SEQUENCE 93 AA; 10812 MW; 9A922E1898D202A4 CRC64;
METPLEKALT TMVTTFHKYS GREGSKLTLS RKELKELIKT ELSLAEKMKE SSIDNLMKSL
DKNSDQEIDF KEYSVFLTTL CMAYNDFFLE DNK
