BEGIN_MOUSE
ID BEGIN_MOUSE Reviewed; 600 AA.
AC Q68EF6;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Brain-enriched guanylate kinase-associated protein;
GN Name=Begain;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 35-600.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-246, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=16452087; DOI=10.1074/mcp.t500041-mcp200;
RA Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.;
RT "Comprehensive identification of phosphorylation sites in postsynaptic
RT density preparations.";
RL Mol. Cell. Proteomics 5:914-922(2006).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-137, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=18034455; DOI=10.1021/pr0701254;
RA Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.;
RT "Large-scale identification and evolution indexing of tyrosine
RT phosphorylation sites from murine brain.";
RL J. Proteome Res. 7:311-318(2008).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-229; SER-246; THR-249;
RP SER-265; SER-483 AND SER-485, AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-380, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryo;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- FUNCTION: May sustain the structure of the postsynaptic density (PSD).
CC -!- SUBUNIT: Interacts with DLG4 and DLGAP1 and forms a ternary complex.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}.
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DR EMBL; AC140111; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC080282; AAH80282.1; -; mRNA.
DR CCDS; CCDS88400.1; -.
DR RefSeq; NP_001156647.1; NM_001163175.1.
DR RefSeq; XP_006516107.1; XM_006516044.2.
DR RefSeq; XP_011242432.1; XM_011244130.2.
DR AlphaFoldDB; Q68EF6; -.
DR SMR; Q68EF6; -.
DR BioGRID; 237646; 4.
DR IntAct; Q68EF6; 4.
DR MINT; Q68EF6; -.
DR STRING; 10090.ENSMUSP00000140393; -.
DR iPTMnet; Q68EF6; -.
DR PhosphoSitePlus; Q68EF6; -.
DR PaxDb; Q68EF6; -.
DR PRIDE; Q68EF6; -.
DR ProteomicsDB; 273554; -.
DR Antibodypedia; 146; 171 antibodies from 27 providers.
DR DNASU; 380785; -.
DR Ensembl; ENSMUST00000238841; ENSMUSP00000158999; ENSMUSG00000040867.
DR GeneID; 380785; -.
DR KEGG; mmu:380785; -.
DR CTD; 57596; -.
DR MGI; MGI:3044626; Begain.
DR VEuPathDB; HostDB:ENSMUSG00000040867; -.
DR eggNOG; ENOG502QUGW; Eukaryota.
DR GeneTree; ENSGT00940000161760; -.
DR InParanoid; Q68EF6; -.
DR OrthoDB; 443898at2759; -.
DR PhylomeDB; Q68EF6; -.
DR BioGRID-ORCS; 380785; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Begain; mouse.
DR PRO; PR:Q68EF6; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q68EF6; protein.
DR Bgee; ENSMUSG00000040867; Expressed in hypothalamus and 67 other tissues.
DR ExpressionAtlas; Q68EF6; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030425; C:dendrite; ISO:MGI.
DR GO; GO:0098978; C:glutamatergic synapse; IDA:SynGO.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043025; C:neuronal cell body; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0098794; C:postsynapse; ISO:MGI.
DR GO; GO:0098793; C:presynapse; IDA:MGI.
DR GO; GO:0045202; C:synapse; ISO:MGI.
DR GO; GO:0098817; P:evoked excitatory postsynaptic potential; IDA:MGI.
DR GO; GO:0098962; P:regulation of postsynaptic neurotransmitter receptor activity; IDA:SynGO.
DR InterPro; IPR033584; BEGAIN.
DR InterPro; IPR043441; Tjap1/BEGAIN.
DR PANTHER; PTHR28664; PTHR28664; 1.
DR PANTHER; PTHR28664:SF2; PTHR28664:SF2; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Membrane; Methylation; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..600
FT /note="Brain-enriched guanylate kinase-associated protein"
FT /id="PRO_0000064905"
FT REGION 192..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 298..317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 537..590
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 204..218
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 571..590
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q9BUH8"
FT MOD_RES 137
FT /note="Phosphotyrosine"
FT /evidence="ECO:0007744|PubMed:18034455"
FT MOD_RES 200
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BUH8"
FT MOD_RES 229
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 246
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:16452087,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 249
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 265
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 372
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BUH8"
FT MOD_RES 380
FT /note="Asymmetric dimethylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 463
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BUH8"
FT MOD_RES 473
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BUH8"
FT MOD_RES 483
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 485
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 508
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BUH8"
FT MOD_RES 510
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BUH8"
FT MOD_RES 514
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BUH8"
FT MOD_RES 560
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BUH8"
FT MOD_RES 570
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BUH8"
SQ SEQUENCE 600 AA; 65309 MW; 0BECA620CF2971D0 CRC64;
MEKLSALQEQ KGELRKRLSY TTHKLEKLET EFDSTRHYLE IELRRAQEEL DKVTEKLRRI
QSNYMALQRI NQELEDKLYR MGQHYEEEKR AMSHEIVALN SHLLEAKVTI DKLSEDNELY
RKDCNLAAQL LQCSQTYGRV HKVSELPSDF QQRVSLHMEK HGCSLPSALC HPAYADSVPT
CVIAKVLEKP DPGSLSSRMS DASARDLGYR DGVEKSGPRP PYKGDIYCSD PALYCPDERE
HARRPSVDTP VTDVGFLRAQ NSTDSAAEEE EEAEAAAFPE AYRREAYQGY AASLPTSSSY
SSFSATSEEK EHAQAGTLTA SQQAIYLSSR DEFFNRKPSA TYGSGPRFAK AASTLGSPLE
AQVAPGFART VSPYPAEPYR YPASPGPQQA LMPPNLWSLR AKPSGNRLAG EDIRGQWRPV
SVEDVGAYSY QAGAAAGRAA SPCNYSERYY GGGGGGGAAG GGSPGDKAEG RASPLYATYK
ADSFSEGDDL SQGHLAEPCF LRAGGDLSLS PSRSADALAG YAASDGDGDR LRVQLCGAGS
SPEPEHGSRE SLEPSSMEAS PEMHPPTRLS PQQAFPRTGG SGLSRKDSLT KAQLYGTLLN
