S10AC_PIG
ID S10AC_PIG Reviewed; 92 AA.
AC P80310;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Protein S100-A12;
DE AltName: Full=Calgranulin-C;
DE Short=CAGC;
DE AltName: Full=Extracellular newly identified RAGE-binding protein;
DE Short=EN-RAGE;
DE AltName: Full=S100 calcium-binding protein A12;
GN Name=S100A12;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP PROTEIN SEQUENCE OF 2-92.
RC TISSUE=Granulocyte;
RX PubMed=7961855; DOI=10.1016/s0021-9258(19)61996-4;
RA Dell'Angelica E.C., Schleicher C.H., Santome J.A.;
RT "Primary structure and binding properties of calgranulin C, a novel S100-
RT like calcium-binding protein from pig granulocytes.";
RL J. Biol. Chem. 269:28929-28936(1994).
CC -!- FUNCTION: S100A12 is a calcium-, zinc- and copper-binding protein which
CC plays a prominent role in the regulation of inflammatory processes and
CC immune response. Its pro-inflammatory activity involves recruitment of
CC leukocytes, promotion of cytokine and chemokine production, and
CC regulation of leukocyte adhesion and migration. Acts as an alarmin or a
CC danger associated molecular pattern (DAMP) molecule and stimulates
CC innate immune cells via binding to receptor for advanced glycation
CC endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-
CC kappa-B signaling pathways leading to production of pro-inflammatory
CC cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1.
CC Acts as a monocyte and mast cell chemoattractant. Can stimulate mast
CC cell degranulation and activation which generates chemokines, histamine
CC and cytokines inducing further leukocyte recruitment to the sites of
CC inflammation. Can inhibit the activity of matrix metalloproteinases;
CC MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. Homooligomer (tetramer or hexamer) in the presence
CC of calcium, zinc and copper ions. Interacts with AGER and both calcium
CC and zinc are essential for the interaction (By similarity). Interacts
CC with CACYBP in a calcium-dependent manner (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC Cytoplasm, cytoskeleton {ECO:0000250}. Cell membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}. Note=Predominantly localized
CC in the cytoplasm. Upon elevation of the intracellular calcium level,
CC translocated from the cytoplasm to the cytoskeleton and the cell
CC membrane. Upon neutrophil activation is secreted via a microtubule-
CC mediated, alternative pathway (By similarity). {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Found essentially in granulocytes with small
CC amounts found in lymphocytes.
CC -!- DOMAIN: The hinge domain contributes significantly to its chemotactic
CC properties. {ECO:0000250}.
CC -!- MISCELLANEOUS: In the absence of zinc binds one calcium ion per
CC molecule, in the presence of zinc binds two calcium ions per molecule.
CC -!- SIMILARITY: Belongs to the S-100 family. {ECO:0000305}.
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DR PIR; A55406; A55406.
DR RefSeq; NP_001153744.1; NM_001160272.1.
DR AlphaFoldDB; P80310; -.
DR SMR; P80310; -.
DR STRING; 9823.ENSSSCP00000007024; -.
DR PaxDb; P80310; -.
DR PeptideAtlas; P80310; -.
DR PRIDE; P80310; -.
DR Ensembl; ENSSSCT00005027709; ENSSSCP00005016877; ENSSSCG00005017529.
DR Ensembl; ENSSSCT00015028578; ENSSSCP00015011189; ENSSSCG00015021486.
DR Ensembl; ENSSSCT00015028675; ENSSSCP00015011241; ENSSSCG00015021486.
DR Ensembl; ENSSSCT00070056169; ENSSSCP00070047719; ENSSSCG00070027991.
DR GeneID; 100301483; -.
DR KEGG; ssc:100301483; -.
DR CTD; 6283; -.
DR eggNOG; ENOG502SA01; Eukaryota.
DR HOGENOM; CLU_138624_6_2_1; -.
DR InParanoid; P80310; -.
DR OMA; TKLEDHM; -.
DR OrthoDB; 1521098at2759; -.
DR TreeFam; TF332727; -.
DR Reactome; R-SSC-445989; TAK1-dependent IKK and NF-kappa-B activation.
DR Reactome; R-SSC-6798695; Neutrophil degranulation.
DR Reactome; R-SSC-879415; Advanced glycosylation endproduct receptor signaling.
DR Reactome; R-SSC-933542; TRAF6 mediated NF-kB activation.
DR Proteomes; UP000008227; Unplaced.
DR Proteomes; UP000314985; Chromosome 4.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IDA:UniProtKB.
DR GO; GO:0048306; F:calcium-dependent protein binding; IBA:GO_Central.
DR GO; GO:0050786; F:RAGE receptor binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IDA:UniProtKB.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR GO; GO:0006954; P:inflammatory response; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB signaling; IBA:GO_Central.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR001751; S100/CaBP7/8-like_CS.
DR InterPro; IPR013787; S100_Ca-bd_sub.
DR Pfam; PF01023; S_100; 1.
DR SMART; SM00054; EFh; 1.
DR SMART; SM01394; S_100; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
DR PROSITE; PS00303; S100_CABP; 1.
PE 1: Evidence at protein level;
KW Calcium; Cell membrane; Copper; Cytoplasm; Cytoskeleton;
KW Direct protein sequencing; Immunity; Inflammatory response;
KW Innate immunity; Membrane; Metal-binding; Reference proteome; Repeat;
KW Secreted; Zinc.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:7961855"
FT CHAIN 2..92
FT /note="Protein S100-A12"
FT /id="PRO_0000144016"
FT DOMAIN 13..48
FT /note="EF-hand 1"
FT /evidence="ECO:0000305"
FT DOMAIN 49..84
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 38..53
FT /note="Hinge domain"
FT /evidence="ECO:0000250"
FT BINDING 16
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000250|UniProtKB:P80511"
FT BINDING 16
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P80511"
FT BINDING 19
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /ligand_note="low affinity"
FT /evidence="ECO:0000250|UniProtKB:P80511"
FT BINDING 24
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /ligand_note="low affinity"
FT /evidence="ECO:0000250|UniProtKB:P80511"
FT BINDING 26
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000250|UniProtKB:P80511"
FT BINDING 26
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P80511"
FT BINDING 27
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /ligand_note="low affinity"
FT /evidence="ECO:0000250|UniProtKB:P80511"
FT BINDING 32
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /ligand_note="low affinity"
FT /evidence="ECO:0000250|UniProtKB:P80511"
FT BINDING 62
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /ligand_note="high affinity"
FT /evidence="ECO:0000250|UniProtKB:P80511"
FT BINDING 64
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /ligand_note="high affinity"
FT /evidence="ECO:0000250|UniProtKB:P80511"
FT BINDING 66
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /ligand_note="high affinity"
FT /evidence="ECO:0000250|UniProtKB:P80511"
FT BINDING 68
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /ligand_note="high affinity"
FT /evidence="ECO:0000250|UniProtKB:P80511"
FT BINDING 73
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /ligand_note="high affinity"
FT /evidence="ECO:0000250|UniProtKB:P80511"
FT BINDING 86
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000250|UniProtKB:P80511"
FT BINDING 86
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P80511"
FT BINDING 90
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000250|UniProtKB:P80511"
FT BINDING 90
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P80511"
SQ SEQUENCE 92 AA; 10745 MW; D4D9B428DE64AB53 CRC64;
MTKLEDHLEG IINIFHQYSV RLGHYDTLIK RELKQLITKE LPNTLKNTKD QGTIDKIFQN
LDANQDEQVS FKEFVVLVTD VLITAHDNIH KE
