S12A3_PSEAM
ID S12A3_PSEAM Reviewed; 1023 AA.
AC P55019;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Solute carrier family 12 member 3;
DE AltName: Full=Na-Cl symporter;
DE AltName: Full=Thiazide-sensitive sodium-chloride cotransporter;
GN Name=slc12a3; Synonyms=tsc;
OS Pseudopleuronectes americanus (Winter flounder) (Pleuronectes americanus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Carangaria; Pleuronectiformes; Pleuronectoidei; Pleuronectidae;
OC Pseudopleuronectes.
OX NCBI_TaxID=8265;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Urinary bladder urothelium;
RX PubMed=8464884; DOI=10.1073/pnas.90.7.2749;
RA Gamba G., Saltzberg S.N., Lombardi M., Miyanoshita A., Lytton J.,
RA Hediger M.A., Brenner B.M., Hebert S.C.;
RT "Primary structure and functional expression of a cDNA encoding the
RT thiazide-sensitive, electroneutral sodium-chloride cotransporter.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:2749-2753(1993).
CC -!- FUNCTION: Electrically silent transporter system. Mediates sodium and
CC chloride reabsorption.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Urinary bladder.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family. {ECO:0000305}.
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DR EMBL; L11615; AAA49272.1; -; mRNA.
DR PIR; A47296; A47296.
DR AlphaFoldDB; P55019; -.
DR SMR; P55019; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProt.
DR GO; GO:0015377; F:cation:chloride symporter activity; IEA:InterPro.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR013612; AA_permease_N.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR002948; SLC12A3.
DR InterPro; IPR004842; SLC12A_fam.
DR PANTHER; PTHR11827; PTHR11827; 1.
DR PANTHER; PTHR11827:SF9; PTHR11827:SF9; 1.
DR Pfam; PF00324; AA_permease; 1.
DR Pfam; PF08403; AA_permease_N; 1.
DR Pfam; PF03522; SLC12; 2.
DR PRINTS; PR01230; NACLTRNSPORT.
DR TIGRFAMs; TIGR00930; 2a30; 1.
PE 2: Evidence at transcript level;
KW Chloride; Glycoprotein; Ion transport; Membrane; Sodium; Sodium transport;
KW Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1023
FT /note="Solute carrier family 12 member 3"
FT /id="PRO_0000178029"
FT TOPO_DOM 1..132
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..215
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 259..279
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 280..283
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..304
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 337..357
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 358..374
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 375..395
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 452..472
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 473..510
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 511..531
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 577..597
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 598..745
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 746..766
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 831..851
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 852..1023
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 91..131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 403
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 414
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 432
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 543
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1023 AA; 112341 MW; 69AE2D53B8F84D89 CRC64;
MELPGDGVHL ASYGPRAPQL AVNGKAPAGG LDGSGYYQCY RDGSSVASRG SDAPTGYDTV
DAPPHYDFYA NTEVWGRRRR VRPSLYQLSV DPEQDDFKPP MYEETAGERM GGGDSSEEEE
EEHKEPPPEP PRFGWVQGVM IRCMLNIWGV ILYLRLPWIT AQAGIGLTWV IILLSSFITG
ITGLSTSAIA TNGKVKGGGT YFLISRSLGP ELGGSIGLIF AFANAVAVAM HTVGFAETVT
DLMRENGVVM VDPINDIRIV GVITVTCLLG ISMAGMEWES KAQVLFFLVI MVSFVNYIVG
TIIPASPQKQ AKGFFSYKAE IFAANFVPGW RGKEGSFFGM FSIFFPSATG ILAGANISGD
LKDPTVAIPR GTLMAIFWTT ISYLIISATI GACVVRDASG ELNDTLSYSS SSENCSGLAC
QYRWDFSECI KNNTCKHGIM NYYQSMSLVS AFAPLISAGI FGATLSSALA CLVSAPKVFQ
CLCKDQLYPL IGFFGKGYGK NAEPLRAYLL TYVIAVCFVL IAELNTIAPI ISNFFLCSYA
LINFSCFHAS VTNSPGWRPS FRFYSKWLSL LGAVCCVVIM FLLTWWAALI AFGVVFFLLG
YTLYKKPAVN WGSSVQASSY SMALNQCVGL NQVEDHVKNY RPQCLVLTGP PCCRPALVDL
VGCLTKRLSL MMCGHVVTAG PSPVSERHVT WLNQRKVRSF YRGVVAADLR SGVNMLLQGA
GLGRIKPNVL LMGFKKDWGC DSPQAAHHYI GILHDAFDLH YGVCVLRVKE GLDASHPPQC
HVNPGFDGGP ESINTVCAPA CVQTSVTSSV SMDPDPQPSS VFQKKQGKKT IDIYWLSDDG
GLTLLLPYLL TRRKRWAGCK VRVFVGGDTD KKEEQKEEVL ALIKKFRLGF HDVEVLPDIH
QPPQPGNVDH FEDSVNRFRL ETNPKQDSDS GPQQQQQEEP WMITEQDLER NRAKSLRQIR
LNEVLQVHSR EAALIVITMP VGRRGVCPST LFLAWLDVLS RDLRPPVLLV RGNQENVLTF
YCQ
