S12A8_MOUSE
ID S12A8_MOUSE Reviewed; 705 AA.
AC Q8VI23; Q3TNQ9; Q8K0M9;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Solute carrier family 12 member 8;
DE AltName: Full=Cation-chloride cotransporter 9;
GN Name=Slc12a8; Synonyms=Ccc9;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J;
RA Mount D.B.;
RT "Cloning and characterization of mouse CCC9 (SLC12A9), a new member of the
RT cation-chloride cotransporter gene family.";
RL Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC STRAIN=C57BL/6J; TISSUE=Ovary;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=FVB/N; TISSUE=Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Cation/chloride cotransporter that may play a role in the
CC control of keratinocyte proliferation. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q8VI23-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VI23-2; Sequence=VSP_028335;
CC Name=3;
CC IsoId=Q8VI23-3; Sequence=VSP_028334, VSP_028336;
CC Name=4;
CC IsoId=Q8VI23-4; Sequence=VSP_028333, VSP_028336;
CC -!- SIMILARITY: Belongs to the SLC12A transporter family. {ECO:0000305}.
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DR EMBL; AF319951; AAL37178.1; -; mRNA.
DR EMBL; AK165080; BAE38029.1; -; mRNA.
DR EMBL; AC121570; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC127412; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC030926; AAH30926.1; -; mRNA.
DR CCDS; CCDS37317.1; -. [Q8VI23-3]
DR CCDS; CCDS49834.1; -. [Q8VI23-1]
DR RefSeq; NP_001077371.1; NM_001083902.1. [Q8VI23-3]
DR RefSeq; NP_599012.2; NM_134251.2. [Q8VI23-1]
DR RefSeq; XP_006521883.1; XM_006521820.2.
DR AlphaFoldDB; Q8VI23; -.
DR SMR; Q8VI23; -.
DR STRING; 10090.ENSMUSP00000113633; -.
DR iPTMnet; Q8VI23; -.
DR PhosphoSitePlus; Q8VI23; -.
DR PaxDb; Q8VI23; -.
DR PRIDE; Q8VI23; -.
DR ProteomicsDB; 253341; -. [Q8VI23-1]
DR ProteomicsDB; 253342; -. [Q8VI23-2]
DR ProteomicsDB; 253343; -. [Q8VI23-3]
DR ProteomicsDB; 253344; -. [Q8VI23-4]
DR Antibodypedia; 33008; 39 antibodies from 17 providers.
DR DNASU; 171286; -.
DR Ensembl; ENSMUST00000059056; ENSMUSP00000062337; ENSMUSG00000035506. [Q8VI23-1]
DR Ensembl; ENSMUST00000119173; ENSMUSP00000113633; ENSMUSG00000035506. [Q8VI23-3]
DR Ensembl; ENSMUST00000121925; ENSMUSP00000112439; ENSMUSG00000035506. [Q8VI23-1]
DR GeneID; 171286; -.
DR KEGG; mmu:171286; -.
DR UCSC; uc007zad.1; mouse. [Q8VI23-1]
DR UCSC; uc007zae.1; mouse. [Q8VI23-3]
DR UCSC; uc007zag.1; mouse. [Q8VI23-4]
DR CTD; 84561; -.
DR MGI; MGI:2443672; Slc12a8.
DR VEuPathDB; HostDB:ENSMUSG00000035506; -.
DR eggNOG; KOG2083; Eukaryota.
DR GeneTree; ENSGT00940000160130; -.
DR HOGENOM; CLU_017440_1_1_1; -.
DR InParanoid; Q8VI23; -.
DR OMA; WYSGFCN; -.
DR OrthoDB; 792706at2759; -.
DR PhylomeDB; Q8VI23; -.
DR TreeFam; TF313191; -.
DR BioGRID-ORCS; 171286; 4 hits in 75 CRISPR screens.
DR ChiTaRS; Slc12a8; mouse.
DR PRO; PR:Q8VI23; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q8VI23; protein.
DR Bgee; ENSMUSG00000035506; Expressed in gastrula and 92 other tissues.
DR ExpressionAtlas; Q8VI23; baseline and differential.
DR Genevisible; Q8VI23; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015379; F:potassium:chloride symporter activity; IBA:GO_Central.
DR GO; GO:0006884; P:cell volume homeostasis; IBA:GO_Central.
DR GO; GO:0055064; P:chloride ion homeostasis; IBA:GO_Central.
DR GO; GO:1902476; P:chloride transmembrane transport; IBA:GO_Central.
DR GO; GO:0055075; P:potassium ion homeostasis; IBA:GO_Central.
DR GO; GO:1990573; P:potassium ion import across plasma membrane; IBA:GO_Central.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR030343; SLC12A8.
DR InterPro; IPR004842; SLC12A_fam.
DR PANTHER; PTHR11827; PTHR11827; 1.
DR PANTHER; PTHR11827:SF6; PTHR11827:SF6; 1.
DR Pfam; PF00324; AA_permease; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloride; Ion transport; Membrane; Potassium;
KW Potassium transport; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..705
FT /note="Solute carrier family 12 member 8"
FT /id="PRO_0000305288"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 306..326
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 368..388
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 390..410
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 587..607
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 612..632
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 472..512
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..246
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028333"
FT VAR_SEQ 1..31
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_028334"
FT VAR_SEQ 1..6
FT /note="MAQRSP -> MSQV (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_028335"
FT VAR_SEQ 304..352
FT /note="Missing (in isoform 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_028336"
FT CONFLICT 183
FT /note="L -> P (in Ref. 1; AAL37178)"
FT /evidence="ECO:0000305"
FT CONFLICT 236
FT /note="F -> L (in Ref. 1; AAL37178)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 705 AA; 77020 MW; 0AF9193CB067D315 CRC64;
MAQRSPQELF HEAAQQGILA QPQPWWKIQL FMWEPVLFGT WDGVFTSCMI NIFGVVLFLR
TGWLVGNTGV LLGLLLVSFV VLVALITVLS GIGVAEHGGI SSGGVYSMIS SVLGGQMGGT
VGLLYVFGQC VAGAMYITGF AESISDLLGL GDIWAVRGIS VAVLLALLGI NLAGVKWIIR
LQLLLLLLLA VSTLDFVVGS FTHLDPEHGF IGYSPELLQS NILPEYSPGE SFFTVFGVFF
PAATGVMAGF NMGGDLRDPA DSVPLGSLAA VGVSWFLYII FAFLLGAVCT REALRSDFLI
AEKVSLVGFL FLLGLYISSL ASCMGGLYGA PRILQCIAQD KVIPALAFLA NGKGPNKTPV
AAICLTSLVT MAFVLVGQVN VLAPVVTINF MLTYIMVDYS YFALSMAHCG LAPSPEPVPR
QGPDTLHCSE HLLQDRAPSY GSDVPARSLS EGTLLEFTKD MDQFLQPIEE LESRQLGSRE
GNNPKNQKRK GKKGAKQTLQ DSFLLDPGSP LSFPTRTSER LSVAFCGEQE SYQKQQTSRS
ESHDHLVPDL RNQPRVNRED FFLKCRLQEQ EIQRRPSVFY ACMCNPWVSL LGALASLLIM
FVIQWLYTLA SMGVAALVYF YIGQASPGLY LGSASNFSFF QWMKSFLVPS CRSLRSAQEQ
IILAPSPAKV DMAMTQLTQD NADFATRDRY HHSSFLSREQ LMPPY
