S12A8_RAT
ID S12A8_RAT Reviewed; 707 AA.
AC Q8CJI3; Q8CJI2;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=Solute carrier family 12 member 8;
DE AltName: Full=Cation-chloride cotransporter 9;
GN Name=Slc12a8; Synonyms=Ccc9;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RA Ishibashi K., Imai M.;
RT "Molecular cloning of a new member of cation chloride cotransporters.";
RL Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Cation/chloride cotransporter that may play a role in the
CC control of keratinocyte proliferation. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=ccc9a;
CC IsoId=Q8CJI3-1; Sequence=Displayed;
CC Name=2; Synonyms=ccc9b;
CC IsoId=Q8CJI3-2; Sequence=VSP_028337, VSP_028338, VSP_028339;
CC -!- SIMILARITY: Belongs to the SLC12A transporter family. {ECO:0000305}.
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DR EMBL; AB024430; BAC20264.1; -; mRNA.
DR EMBL; AB024431; BAC20265.1; -; mRNA.
DR RefSeq; NP_705889.1; NM_153625.1. [Q8CJI3-1]
DR AlphaFoldDB; Q8CJI3; -.
DR SMR; Q8CJI3; -.
DR STRING; 10116.ENSRNOP00000002439; -.
DR PaxDb; Q8CJI3; -.
DR GeneID; 266733; -.
DR KEGG; rno:266733; -.
DR UCSC; RGD:628730; rat. [Q8CJI3-1]
DR CTD; 84561; -.
DR RGD; 628730; Slc12a8.
DR eggNOG; KOG2083; Eukaryota.
DR InParanoid; Q8CJI3; -.
DR OrthoDB; 792706at2759; -.
DR PhylomeDB; Q8CJI3; -.
DR PRO; PR:Q8CJI3; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015379; F:potassium:chloride symporter activity; IBA:GO_Central.
DR GO; GO:0006884; P:cell volume homeostasis; IBA:GO_Central.
DR GO; GO:0055064; P:chloride ion homeostasis; IBA:GO_Central.
DR GO; GO:1902476; P:chloride transmembrane transport; IBA:GO_Central.
DR GO; GO:0055075; P:potassium ion homeostasis; IBA:GO_Central.
DR GO; GO:1990573; P:potassium ion import across plasma membrane; IBA:GO_Central.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR030343; SLC12A8.
DR InterPro; IPR004842; SLC12A_fam.
DR PANTHER; PTHR11827; PTHR11827; 1.
DR PANTHER; PTHR11827:SF6; PTHR11827:SF6; 1.
DR Pfam; PF00324; AA_permease; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloride; Ion transport; Membrane; Potassium;
KW Potassium transport; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..707
FT /note="Solute carrier family 12 member 8"
FT /id="PRO_0000305289"
FT TRANSMEM 36..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..251
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..295
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 307..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..380
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 579..600
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 606..623
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 469..489
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 354..379
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_028337"
FT VAR_SEQ 594..611
FT /note="ALASLLIMFVIQWLYTLA -> EPEIHPGANHLGTVPSQG (in isoform
FT 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_028338"
FT VAR_SEQ 612..707
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_028339"
FT CONFLICT 431
FT /note="E -> L (in Ref. 1; BAC20265)"
FT /evidence="ECO:0000305"
FT CONFLICT 433
FT /note="L -> R (in Ref. 1; BAC20265)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 707 AA; 77073 MW; 96A01BA7A04EDC57 CRC64;
MAQRSPQELF HEAAQQGVLA RSQPWWKIQL FMWEPVLFGT WDGVFTSCMI NIFGVVLFLR
TGWLVGNTGV LLGLLLVSFV ILVALITVLS GIGVAEHGWM GSGGVYSMIS SVLGGQMGGT
VGLLYVFGQC VAGAMYLTGF AESISDLLGL GNIWAVRGIS VAVLLALLGI NLAGVKWIIR
LQLLLLSLLA VSTLDFVVGS FTHLDPEHGF IGYSPELLQS NILPEYSPGE SFFTVFGVFF
PAATGVMAGF NMGGDLREPA DQHTPRLPSS CWVSRGFSNI IFAFLLGAVC TREALRSDFL
IAEKVSLVGF LFLLGLYISS LASCMGGLYG APRILQCIAQ DKVIPALAFL GNGKGPNKTP
VAAICLTSLV TMAFVLVGQV NVLAPVVTIN FMLTYIAVDY SYFALSMAHC GLAQSPEPMP
RQGPDALHCS EHLLQDKAPS YGSDTPAGSL SEGTLLEFTK DMDQLLQPIG GLESQPRSRE
VNQVLKSQKH KCKKDAKQTL QDSFLLDAES PLSFSMGASE RLSVASCGEQ ESYQNQQTSR
SESHDHLVPD LCTQPRANGE DFFLKCRLQE QQVQRRPSAF YVCMCNPWVS LLGALASLLI
MFVIQWLYTL ASMGVAALVY LYIGQASPGL YLGSASNFSF FQWMKSFLVP SCRSLRSTRE
QIILAPSPAK VDMAMTQAYP GQWQTSATRE RYHRSSFLSR EQLMPPY
