S130A_ARATH
ID S130A_ARATH Reviewed; 1214 AA.
AC P0DKL4; Q570A5; Q9LD60;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2017, sequence version 1.
DT 03-AUG-2022, entry version 33.
DE RecName: Full=Spliceosome-associated protein 130 A {ECO:0000303|PubMed:21680607};
DE Short=AtSAP130a {ECO:0000303|PubMed:21680607};
DE Short=SAP 130 A {ECO:0000303|PubMed:21680607};
DE AltName: Full=Pre-mRNA-splicing factor SF3b 130 kDa subunit A {ECO:0000305};
DE Short=SF3b130 A {ECO:0000305};
GN Name=SAP130A {ECO:0000303|PubMed:21680607};
GN OrderedLocusNames=At3g55200 {ECO:0000312|Araport:AT3G55200};
GN ORFNames=T26I12.80 {ECO:0000312|EMBL:CAB75754.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1050-1214.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=15987817; DOI=10.1261/rna.2440305;
RA Lorkovic Z.J., Lehner R., Forstner C., Barta A.;
RT "Evolutionary conservation of minor U12-type spliceosome between plants and
RT humans.";
RL RNA 11:1095-1107(2005).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=21680607; DOI=10.1093/pcp/pcr077;
RA Aki S., Nakai H., Aoyama T., Oka A., Tsuge T.;
RT "AtSAP130/AtSF3b-3 function is required for reproduction in Arabidopsis
RT thaliana.";
RL Plant Cell Physiol. 52:1330-1339(2011).
CC -!- FUNCTION: Subunit of the splicing factor SF3B required for 'A' complex
CC assembly formed by the stable binding of U2 snRNP to the branchpoint
CC sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B
CC complex upstream of the branch site is essential, it may anchor U2
CC snRNP to the pre-mRNA. May also be involved in the assembly of the 'E'
CC complex. Belongs also to the minor U12-dependent spliceosome, which is
CC involved in the splicing of rare class of nuclear pre-mRNA intron (By
CC similarity). Required for pollen and ovule development, especially
CC during the transition from microspore to the bicellular stage in pollen
CC development. Involved in the accumulation of QRT1 and QRT3
CC (PubMed:21680607). {ECO:0000250|UniProtKB:Q15393,
CC ECO:0000269|PubMed:21680607}.
CC -!- SUBUNIT: Identified in the spliceosome C complex. Component of the
CC U11/U12 snRNPs that are part of the U12-type spliceosome. Component of
CC splicing factor SF3B complex. {ECO:0000250|UniProtKB:Q15393}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q15393}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in roots, leaves,
CC inflorescence and, to a lower extent, in siliques.
CC {ECO:0000269|PubMed:21680607}.
CC -!- DISRUPTION PHENOTYPE: The double mutant sap130a sap130b displays a
CC slight reduction in the size of aerial organs and in the number of
CC lateral roots, and is impaired in reproduction due to a reduced
CC production of viable pollen and impaired female reproductive organs.
CC Defect in the transition from microspore to the bicellular stage in
CC pollen development. Reduced expression of QRT1 and QRT3.
CC {ECO:0000269|PubMed:21680607}.
CC -!- SIMILARITY: Belongs to the RSE1 family. {ECO:0000305}.
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DR EMBL; AL132954; CAB75754.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79352.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM65660.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM65661.1; -; Genomic_DNA.
DR EMBL; AK220805; BAD94072.1; -; mRNA.
DR PIR; T47659; T47659.
DR RefSeq; NP_001319757.1; NM_001339727.1.
DR RefSeq; NP_001327611.1; NM_001339728.1.
DR RefSeq; NP_567015.1; NM_115378.4.
DR RefSeq; NP_567016.1; NM_115380.3.
DR AlphaFoldDB; P0DKL4; -.
DR SMR; P0DKL4; -.
DR STRING; 3702.AT3G55200.1; -.
DR PRIDE; P0DKL4; -.
DR EnsemblPlants; AT3G55200.1; AT3G55200.1; AT3G55200.
DR EnsemblPlants; AT3G55200.2; AT3G55200.2; AT3G55200.
DR EnsemblPlants; AT3G55200.3; AT3G55200.3; AT3G55200.
DR EnsemblPlants; AT3G55220.1; AT3G55220.1; AT3G55220.
DR GeneID; 824686; -.
DR GeneID; 824688; -.
DR Gramene; AT3G55200.1; AT3G55200.1; AT3G55200.
DR Gramene; AT3G55200.2; AT3G55200.2; AT3G55200.
DR Gramene; AT3G55200.3; AT3G55200.3; AT3G55200.
DR Gramene; AT3G55220.1; AT3G55220.1; AT3G55220.
DR KEGG; ath:AT3G55200; -.
DR KEGG; ath:AT3G55220; -.
DR Araport; AT3G55200; -.
DR eggNOG; KOG1898; Eukaryota.
DR OrthoDB; 97116at2759; -.
DR PRO; PR:P0DKL4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; P0DKL4; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005686; C:U2 snRNP; IBA:GO_Central.
DR GO; GO:0030620; F:U2 snRNA binding; IBA:GO_Central.
DR GO; GO:0055046; P:microgametogenesis; IMP:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0048481; P:plant ovule development; IMP:UniProtKB.
DR GO; GO:0009555; P:pollen development; IMP:UniProtKB.
DR GO; GO:0009846; P:pollen germination; IMP:UniProtKB.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR004871; Cleavage/polyA-sp_fac_asu_C.
DR InterPro; IPR018846; Cleavage/polyA-sp_fac_asu_N.
DR InterPro; IPR011044; Quino_amine_DH_bsu.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF03178; CPSF_A; 1.
DR Pfam; PF10433; MMS1_N; 1.
DR SUPFAM; SSF50969; SSF50969; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 2: Evidence at transcript level;
KW Nucleus; Reference proteome.
FT CHAIN 1..1214
FT /note="Spliceosome-associated protein 130 A"
FT /id="PRO_0000439506"
FT REGION 817..848
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 1154
FT /note="C -> R (in Ref. 3; BAD94072)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1214 AA; 134967 MW; F21E86C930D11FC9 CRC64;
MYLYSLTLQQ ATGIVCAING NFSGGKTQEI AVARGKILDL LRPDENGKIQ TIHSVEVFGA
IRSLAQFRLT GAQKDYIVVG SDSGRIVILE YNKEKNVFDK VHQETFGKSG CRRIVPGQYV
AVDPKGRAVM IGACEKQKLV YVLNRDTTAR LTISSPLEAH KSHTICYSLC GVDCGFDNPI
FAAIELDYSE ADQDPTGQAA SEAQKHLTFY ELDLGLNHVS RKWSNPVDNG ANMLVTVPGG
ADGPSGVLVC AENFVIYMNQ GHPDVRAVIP RRTDLPAERG VLVVSAAVHK QKTMFFFLIQ
TEYGDVFKVT LDHNGDHVSE LKVKYFDTIP VASSICVLKL GFLFSASEFG NHGLYQFQAI
GEEPDVESSS SNLMETEEGF QPVFFQPRRL KNLVRIDQVE SLMPLMDMKV LNIFEEETPQ
IFSLCGRGPR SSLRILRPGL AITEMAVSQL PGQPSAVWTV KKNVSDEFDA YIVVSFTNAT
LVLSIGEQVE EVNDSGFLDT TPSLAVSLIG DDSLMQVHPN GIRHIREDGR INEWRTPGKR
SIVKVGYNRL QVVIALSGGE LIYFEADMTG QLMEVEKHEM SGDVACLDIA PVPEGRKRSR
FLAVGSYDNT VRILSLDPDD CLQILSVQSV SSAPESLLFL EVQASIGGDD GADHPANLFL
NSGLQNGVLF RTVVDMVTGQ LSDSRSRFLG LKPPKLFSIS VRGRSAMLCL SSRPWLGYIH
RGHFHLTPLS YETLEFAAPF SSDQCAEGVV SVAGDALRIF MIDRLGETFN ETVVPLRYTP
RKFVLHPKRK LLVIIESDQG AFTAEEREAA RKECFEAGGV GENGNGNADQ MENGADDEDK
EDPLSDEQYG YPKAESEKWV SCIRVLDPKT ATTTCLLELQ DNEAAYSVCT VNFHDKEYGT
LLAVGTVKGM QFWPKKNLVA GFIHIYRFVE DGKSLELLHK TQVEGVPLAL CQFQGRLLAG
IGPVLRLYDL GKKRLLRKCE NKLFPNTIIS IQTYRDRIYV GDIQESFHYC KYRRDENQLY
IFADDCVPRW LTASHHVDFD TMAGADKFGN VYFVRLPQDL SEEIEEDPTG GKIKWEQGKL
NGAPNKVDEI VQFHVGDVVT CLQKASMIPG GSESIMYGTV MGSIGALHAF TSRDDVDFFS
HLEMHMRQEY PPLCGRDHMA YRSAYFPVKD VIDGDLCEQF PTLPMDLQRK IADELDRTPA
EILKKLEDAR NKII
