S13A3_MOUSE
ID S13A3_MOUSE Reviewed; 600 AA.
AC Q91Y63;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Solute carrier family 13 member 3;
DE AltName: Full=Na(+)/dicarboxylate cotransporter 3;
DE Short=NaDC-3;
DE Short=mNaDC3;
DE AltName: Full=Sodium-dependent high-affinity dicarboxylate transporter 2;
GN Name=Slc13a3; Synonyms=Nadc3, Sdct2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=11287335; DOI=10.1152/ajpcell.2001.280.5.c1215;
RA Pajor A.M., Gangula R., Yao X.;
RT "Cloning and functional characterization of a high-affinity
RT Na(+)/dicarboxylate cotransporter from mouse brain.";
RL Am. J. Physiol. 280:C1215-C1223(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: High-affinity sodium-dicarboxylate cotransporter that accepts
CC a range of substrates with 4-6 carbon atoms, including succinate,
CC alpha-ketoglutarate and N-acetylaspartate. The stoichiometry is
CC probably 3 Na(+) for 1 divalent succinate.
CC {ECO:0000250|UniProtKB:Q8WWT9}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Highly expressed in kidney, and at much lower
CC levels in brain.
CC -!- SIMILARITY: Belongs to the SLC13A/DASS transporter (TC 2.A.47) family.
CC NADC subfamily. {ECO:0000305}.
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DR EMBL; AF306491; AAK49898.1; -; mRNA.
DR EMBL; BC026803; AAH26803.1; -; mRNA.
DR CCDS; CCDS17081.1; -.
DR RefSeq; NP_473396.1; NM_054055.2.
DR AlphaFoldDB; Q91Y63; -.
DR SMR; Q91Y63; -.
DR BioGRID; 227779; 1.
DR STRING; 10090.ENSMUSP00000029208; -.
DR GlyGen; Q91Y63; 2 sites.
DR iPTMnet; Q91Y63; -.
DR PhosphoSitePlus; Q91Y63; -.
DR jPOST; Q91Y63; -.
DR MaxQB; Q91Y63; -.
DR PaxDb; Q91Y63; -.
DR PRIDE; Q91Y63; -.
DR ProteomicsDB; 260965; -.
DR Antibodypedia; 13229; 130 antibodies from 24 providers.
DR DNASU; 114644; -.
DR Ensembl; ENSMUST00000029208; ENSMUSP00000029208; ENSMUSG00000018459.
DR GeneID; 114644; -.
DR KEGG; mmu:114644; -.
DR UCSC; uc008nxs.1; mouse.
DR CTD; 64849; -.
DR MGI; MGI:2149635; Slc13a3.
DR VEuPathDB; HostDB:ENSMUSG00000018459; -.
DR eggNOG; KOG1281; Eukaryota.
DR GeneTree; ENSGT01030000234550; -.
DR InParanoid; Q91Y63; -.
DR OMA; NFSICLM; -.
DR OrthoDB; 389981at2759; -.
DR PhylomeDB; Q91Y63; -.
DR TreeFam; TF312913; -.
DR Reactome; R-MMU-433137; Sodium-coupled sulphate, di- and tri-carboxylate transporters.
DR BioGRID-ORCS; 114644; 2 hits in 75 CRISPR screens.
DR ChiTaRS; Slc13a3; mouse.
DR PRO; PR:Q91Y63; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q91Y63; protein.
DR Bgee; ENSMUSG00000018459; Expressed in adult mammalian kidney and 212 other tissues.
DR ExpressionAtlas; Q91Y63; baseline and differential.
DR Genevisible; Q91Y63; MM.
DR GO; GO:0016323; C:basolateral plasma membrane; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; ISO:MGI.
DR GO; GO:0016020; C:membrane; IDA:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0015139; F:alpha-ketoglutarate transmembrane transporter activity; ISO:MGI.
DR GO; GO:0015137; F:citrate transmembrane transporter activity; ISO:MGI.
DR GO; GO:0005310; F:dicarboxylic acid transmembrane transporter activity; ISO:MGI.
DR GO; GO:0034634; F:glutathione transmembrane transporter activity; ISO:MGI.
DR GO; GO:0015362; F:high-affinity sodium:dicarboxylate symporter activity; IDA:MGI.
DR GO; GO:0015183; F:L-aspartate transmembrane transporter activity; ISO:MGI.
DR GO; GO:0005343; F:organic acid:sodium symporter activity; ISO:MGI.
DR GO; GO:0017153; F:sodium:dicarboxylate symporter activity; ISO:MGI.
DR GO; GO:0015141; F:succinate transmembrane transporter activity; IDA:ARUK-UCL.
DR GO; GO:0098656; P:anion transmembrane transport; IBA:GO_Central.
DR GO; GO:0015810; P:aspartate transmembrane transport; ISO:MGI.
DR GO; GO:0015746; P:citrate transport; ISO:MGI.
DR GO; GO:0006835; P:dicarboxylic acid transport; IDA:MGI.
DR GO; GO:0034775; P:glutathione transmembrane transport; ISO:MGI.
DR GO; GO:0071422; P:succinate transmembrane transport; IDA:ARUK-UCL.
DR GO; GO:0015744; P:succinate transport; ISO:MGI.
DR InterPro; IPR001898; SLC13A/DASS.
DR Pfam; PF00939; Na_sulph_symp; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Ion transport; Membrane; Reference proteome;
KW Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..600
FT /note="Solute carrier family 13 member 3"
FT /id="PRO_0000172493"
FT TOPO_DOM 1..16
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 38..55
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..82
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 104..137
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..229
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 251..278
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 300..336
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 337..357
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 358..372
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 373..393
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 394..422
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT INTRAMEM 423..443
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 444..461
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 462..482
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 483..505
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 506..526
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 527..546
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 547..567
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 568..600
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CARBOHYD 584
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 594
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 600 AA; 66143 MW; 8665D6BAA59AAB51 CRC64;
MAALAALAKK VWSARRLLVL LLVPLALLPI LFALPPKEGR CLYVILLMAV YWCTEALPLS
VTALLPIILF PFMGILPSSK VCPQYFLDTN FLFLSGLIMA SAIEEWNLHR RIALKVLMLV
GVQPARLILG MMVTTSFLSM WLSNTASTAM MLPIASAILK SLFGQREARK DLPREGDEST
AAVQGNGLRT VPTEMQFLAS SEGGHTEDAE APMELPDDSK EEEHRRNIWK GFLISIPYSA
SIGGTATLTG TAPNLILLGQ LKSFFPQCDV VNFGSWFIFA FPLMLLFLLV GWLWISFLYG
GMSWRSWRKK KSKIRADAED QAKAVIQEEF QNLGPIKFAE QAVFILFCTF AILLFSRDPK
FIPGWASLFA PGFVSDAVTG VAIVTILFFF PSQKPSLKWW FDFKAPNSET EPLLSWKKAQ
ETVPWNIILL LGGGFAMAKG CEESGLSAWI GGQLHPLEHV PPLLAVLLIT VVIAFFTEFA
SNTATIIIFL PVLAELAIRL HVHPLYLMIP GTVGCSYAFM LPVSTPPNSI AFSTGHLLVK
DMVRTGLLMN LMGVLLLSLA MNTWAQTIFQ LGTFPDWANT HAANATALPP ALTNNTVQTF
