S13A3_RAT
ID S13A3_RAT Reviewed; 600 AA.
AC Q9Z0Z5;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Solute carrier family 13 member 3;
DE AltName: Full=Na(+)/dicarboxylate cotransporter 3;
DE Short=NaDC-3;
DE Short=rNaDC3;
DE AltName: Full=Sodium-dependent high-affinity dicarboxylate transporter 2;
GN Name=Slc13a3; Synonyms=Nadc3, Sdct2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Placenta;
RX PubMed=9920886; DOI=10.1074/jbc.274.6.3422;
RA Kekuda R., Wang H., Huang W., Pajor A.M., Leibach F.H., Devoe L.D.,
RA Prasad P.D., Ganapathy V.;
RT "Primary structure and functional characteristics of a mammalian sodium-
RT coupled high affinity dicarboxylate transporter.";
RL J. Biol. Chem. 274:3422-3429(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Kidney;
RX PubMed=10207168; DOI=10.1172/jci5392;
RA Chen X., Tsukaguchi H., Chen X.-Z., Berger U.V., Hediger M.A.;
RT "Molecular and functional analysis of SDCT2, a novel rat sodium-dependent
RT dicarboxylate transporter.";
RL J. Clin. Invest. 103:1159-1168(1999).
CC -!- FUNCTION: High-affinity sodium-dicarboxylate cotransporter that accepts
CC a range of substrates with 4-6 carbon atoms, including succinate,
CC alpha-ketoglutarate and N-acetylaspartate. The stoichiometry is
CC probably 3 Na(+) for 1 divalent succinate.
CC {ECO:0000250|UniProtKB:Q8WWT9}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Highly expressed in proximal parts of straight
CC tubules in the kidney. Detected in placenta, in meningeal layers in the
CC brain, and in hepatocytes surrounding the central vein in liver.
CC -!- SIMILARITY: Belongs to the SLC13A/DASS transporter (TC 2.A.47) family.
CC NADC subfamily. {ECO:0000305}.
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DR EMBL; AF081825; AAD16019.1; -; mRNA.
DR EMBL; AF080451; AAD30657.1; -; mRNA.
DR AlphaFoldDB; Q9Z0Z5; -.
DR SMR; Q9Z0Z5; -.
DR STRING; 10116.ENSRNOP00000025843; -.
DR TCDB; 2.A.47.1.4; the divalent anion:na(+) symporter (dass) family.
DR GlyGen; Q9Z0Z5; 2 sites.
DR PaxDb; Q9Z0Z5; -.
DR PRIDE; Q9Z0Z5; -.
DR UCSC; RGD:628786; rat.
DR RGD; 628786; Slc13a3.
DR eggNOG; KOG1281; Eukaryota.
DR InParanoid; Q9Z0Z5; -.
DR PhylomeDB; Q9Z0Z5; -.
DR Reactome; R-RNO-433137; Sodium-coupled sulphate, di- and tri-carboxylate transporters.
DR SABIO-RK; Q9Z0Z5; -.
DR PRO; PR:Q9Z0Z5; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0016323; C:basolateral plasma membrane; ISO:RGD.
DR GO; GO:0016021; C:integral component of membrane; IDA:RGD.
DR GO; GO:0016020; C:membrane; IDA:RGD.
DR GO; GO:0015139; F:alpha-ketoglutarate transmembrane transporter activity; ISO:RGD.
DR GO; GO:0015137; F:citrate transmembrane transporter activity; IDA:ARUK-UCL.
DR GO; GO:0005310; F:dicarboxylic acid transmembrane transporter activity; IDA:RGD.
DR GO; GO:0034634; F:glutathione transmembrane transporter activity; ISO:RGD.
DR GO; GO:0015362; F:high-affinity sodium:dicarboxylate symporter activity; ISO:RGD.
DR GO; GO:0015183; F:L-aspartate transmembrane transporter activity; IDA:RGD.
DR GO; GO:0005343; F:organic acid:sodium symporter activity; IDA:RGD.
DR GO; GO:0017153; F:sodium:dicarboxylate symporter activity; IDA:RGD.
DR GO; GO:0015141; F:succinate transmembrane transporter activity; IDA:ARUK-UCL.
DR GO; GO:0098656; P:anion transmembrane transport; IBA:GO_Central.
DR GO; GO:0015810; P:aspartate transmembrane transport; IDA:RGD.
DR GO; GO:0015746; P:citrate transport; IDA:ARUK-UCL.
DR GO; GO:0006835; P:dicarboxylic acid transport; IDA:RGD.
DR GO; GO:0034775; P:glutathione transmembrane transport; ISO:RGD.
DR GO; GO:0071422; P:succinate transmembrane transport; IDA:ARUK-UCL.
DR GO; GO:0015744; P:succinate transport; IDA:RGD.
DR InterPro; IPR001898; SLC13A/DASS.
DR Pfam; PF00939; Na_sulph_symp; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Ion transport; Membrane; Reference proteome;
KW Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..600
FT /note="Solute carrier family 13 member 3"
FT /id="PRO_0000172494"
FT TOPO_DOM 1..16
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 38..55
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..82
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 104..137
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..229
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 251..278
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 300..336
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 337..357
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 358..372
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 373..393
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 394..422
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT INTRAMEM 423..443
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 444..461
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 462..482
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 483..505
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 506..526
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 527..546
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 547..567
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 568..600
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CARBOHYD 584
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 594
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 600 AA; 66099 MW; 35BC6E0885D7AB3B CRC64;
MAALAALAKK VWSARRLLVL LLVPLALLPI LFALPPKEGR CLYVILLMAV YWCTEALPLS
VTALLPIILF PFMGILPSSK VCPQYFLDTN FLFLSGLIMA SAIEERNLHR RIALKVLMLV
GVQPARLILG MMVTTSFLSM WLSNTASTAM MLPIASAILK SLFGQRDTRK DLPREGEDST
AAVRGNGLRT VPTEMQFLAS SEGGHAEDVE APLELPDDSK EEEHRRNIWK GFLISIPYSA
SIGGTATLTG TAPNLILLGQ LKSFFPQCDV VNFGSWFIFA FPLMLLFLLV GWLWISFLYG
GMSWRGWRKK NSKLQDVAED KAKAVIQEEF QNLGPIKFAE QAVFILFCLF AILLFSRDPK
FIPGWASLFA PGFVSDAVTG VAIVTILFFF PSQKPSLKWW FDFKAPNSET EPLLSWKKAQ
ETVPWNIILL LGGGFAMAKG CEESGLSAWI GGQLHPLEHV PPLLAVLLIT VVIAFFTEFA
SNTATIIIFL PVLAELAIRL HVHPLYLMIP GTVSCSYAFM LPVSTPPNSI AFSTGHLLVK
DMVRTGLLMN LMGVLLLSLA MNTWAQAIFQ LGTFPDWANT HAANVTALPP ALTNNTVQTL
