S14L1_MOUSE
ID S14L1_MOUSE Reviewed; 715 AA.
AC A8Y5H7; A2A9B9; Q6A071; Q99J07; Q9DBQ0;
DT 17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=SEC14-like protein 1 {ECO:0000305};
GN Name=Sec14l1 {ECO:0000312|MGI:MGI:1921386};
GN Synonyms=Kiaa4251 {ECO:0000312|EMBL:BAD32225.1};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=C57BL/6J; TISSUE=Lung;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=129; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 86-715 (ISOFORM 1).
RC TISSUE=Fetal brain;
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May play a role in innate immunity by inhibiting the
CC antiviral RIG-I signaling pathway. In this pathway, functions as a
CC negative regulator of DDX58/RIG-I, the cytoplasmic sensor of viral
CC nucleic acids. Prevents the interaction of DDX58 with MAVS/IPS1, an
CC important step in signal propagation. May also regulate the SLC18A3 and
CC SLC5A7 cholinergic transporters. {ECO:0000250|UniProtKB:Q92503}.
CC -!- SUBUNIT: Interacts with DDX58 (via tandem CARD domain); the interaction
CC is direct. Interacts (via GOLD domain) with SLC18A3; the interaction is
CC direct. Interacts with SLC5A7 (via GOLD domain); the interaction is
CC direct. {ECO:0000250|UniProtKB:Q92503}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q92503}. Golgi
CC apparatus {ECO:0000250|UniProtKB:Q92503}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=A8Y5H7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A8Y5H7-2; Sequence=VSP_058109;
CC Name=3;
CC IsoId=A8Y5H7-3; Sequence=VSP_058110;
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DR EMBL; AK004818; BAB23589.1; -; mRNA.
DR EMBL; AL627205; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466558; EDL34612.1; -; Genomic_DNA.
DR EMBL; BC005766; AAH05766.1; -; mRNA.
DR EMBL; AK172947; BAD32225.1; -; mRNA.
DR CCDS; CCDS25683.1; -. [A8Y5H7-3]
DR CCDS; CCDS48988.1; -. [A8Y5H7-2]
DR CCDS; CCDS48989.1; -. [A8Y5H7-1]
DR RefSeq; NP_001159978.1; NM_001166506.1. [A8Y5H7-1]
DR RefSeq; NP_001159979.1; NM_001166507.1. [A8Y5H7-2]
DR RefSeq; NP_083053.2; NM_028777.3. [A8Y5H7-3]
DR AlphaFoldDB; A8Y5H7; -.
DR SMR; A8Y5H7; -.
DR STRING; 10090.ENSMUSP00000021177; -.
DR iPTMnet; A8Y5H7; -.
DR PhosphoSitePlus; A8Y5H7; -.
DR EPD; A8Y5H7; -.
DR MaxQB; A8Y5H7; -.
DR PaxDb; A8Y5H7; -.
DR PRIDE; A8Y5H7; -.
DR ProteomicsDB; 260966; -. [A8Y5H7-1]
DR ProteomicsDB; 260967; -. [A8Y5H7-2]
DR ProteomicsDB; 260968; -. [A8Y5H7-3]
DR Antibodypedia; 32469; 69 antibodies from 15 providers.
DR DNASU; 74136; -.
DR Ensembl; ENSMUST00000021177; ENSMUSP00000021177; ENSMUSG00000020823. [A8Y5H7-3]
DR Ensembl; ENSMUST00000090433; ENSMUSP00000087916; ENSMUSG00000020823. [A8Y5H7-1]
DR Ensembl; ENSMUST00000103026; ENSMUSP00000099315; ENSMUSG00000020823. [A8Y5H7-2]
DR GeneID; 74136; -.
DR KEGG; mmu:74136; -.
DR UCSC; uc007mna.2; mouse. [A8Y5H7-1]
DR UCSC; uc007mnb.2; mouse.
DR UCSC; uc011yih.1; mouse.
DR CTD; 6397; -.
DR MGI; MGI:1921386; Sec14l1.
DR VEuPathDB; HostDB:ENSMUSG00000020823; -.
DR eggNOG; KOG1471; Eukaryota.
DR GeneTree; ENSGT00940000155386; -.
DR HOGENOM; CLU_023840_0_0_1; -.
DR OMA; NAEWTCF; -.
DR OrthoDB; 1133487at2759; -.
DR TreeFam; TF313988; -.
DR BioGRID-ORCS; 74136; 4 hits in 70 CRISPR screens.
DR ChiTaRS; Sec14l1; mouse.
DR PRO; PR:A8Y5H7; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; A8Y5H7; protein.
DR Bgee; ENSMUSG00000020823; Expressed in submandibular gland and 254 other tissues.
DR Genevisible; A8Y5H7; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0039552; F:RIG-I binding; ISS:UniProtKB.
DR GO; GO:0015871; P:choline transport; ISS:UniProtKB.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0039536; P:negative regulation of RIG-I signaling pathway; ISS:UniProtKB.
DR CDD; cd00170; SEC14; 1.
DR Gene3D; 3.40.525.10; -; 1.
DR InterPro; IPR001251; CRAL-TRIO_dom.
DR InterPro; IPR036865; CRAL-TRIO_dom_sf.
DR InterPro; IPR011074; CRAL/TRIO_N_dom.
DR InterPro; IPR036273; CRAL/TRIO_N_dom_sf.
DR InterPro; IPR009038; GOLD_dom.
DR InterPro; IPR036598; GOLD_dom_sf.
DR InterPro; IPR006797; PRELI/MSF1_dom.
DR Pfam; PF00650; CRAL_TRIO; 1.
DR Pfam; PF03765; CRAL_TRIO_N; 1.
DR Pfam; PF04707; PRELI; 1.
DR SMART; SM01100; CRAL_TRIO_N; 1.
DR SMART; SM00516; SEC14; 1.
DR SUPFAM; SSF101576; SSF101576; 1.
DR SUPFAM; SSF46938; SSF46938; 1.
DR SUPFAM; SSF52087; SSF52087; 1.
DR PROSITE; PS50191; CRAL_TRIO; 1.
DR PROSITE; PS50866; GOLD; 1.
DR PROSITE; PS50904; PRELI_MSF1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Golgi apparatus; Immunity;
KW Innate immunity; Phosphoprotein; Reference proteome;
KW Signal transduction inhibitor.
FT CHAIN 1..715
FT /note="SEC14-like protein 1"
FT /id="PRO_0000435528"
FT DOMAIN 3..175
FT /note="PRELI/MSF1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00158"
FT DOMAIN 319..495
FT /note="CRAL-TRIO"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00056"
FT DOMAIN 521..674
FT /note="GOLD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00096"
FT REGION 1..510
FT /note="Required for interaction and inhibitory function
FT toward DDX58"
FT /evidence="ECO:0000250|UniProtKB:Q92503"
FT MOD_RES 234
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q92503"
FT MOD_RES 586
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q92503"
FT VAR_SEQ 715
FT /note="R -> SE (in isoform 2)"
FT /id="VSP_058109"
FT VAR_SEQ 715
FT /note="R -> RWRFC (in isoform 3)"
FT /id="VSP_058110"
FT CONFLICT 288
FT /note="D -> G (in Ref. 1; BAB23589)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 715 AA; 81216 MW; 4495D06ABFE8E4AF CRC64;
MVQKYQSPVR VYKHPFELIM AAYERRFPTC PLIPMFVDSD TVSEFKSEDG ALHVIERRCK
LDIDAPRLLK KIAGVDYVYF VQKNSLNSRD RTLHIEAHNE TFSNRVIIHE HCCYTVHPEN
EDWTCFEQSA SLDIKSFFGF ESTVEKIAMK HYTSNIKKGK EIIEYYLRQL EEEGITFVPR
WTPPPVGPSE TCSSSKNQVT SAAVLVPDAA AVMEGLSGEN LSSPGTASEP VVGTPDDKLD
ADYIKRYLGD LTPLQESCLI RLRQWLQETH KGKIPKDEHI LRFLRARDFN IDKAREIMCQ
SLTWRKQHQV DYILDTWTPP QVLLDYYAGG WHHHDKDGRP LYVLRLGQMD TKGLVRALGE
EALLRYVLSI NEEGLRRCEE NTKVFGRPIS SWTCLVDLEG LNMRHLWRPG VKALLRIIEV
VEANYPETLG RLLILRAPRV FPVLWTLVSP FIDDNTRRKF LIYAGNDYQG PGGLLDYIDK
EIIPDFLSGE CMCDVPEGGL VPKSLYRTAE ELENEDLKLW TETIYQSASV FKGAPHEILI
QIVDASSVIT WDFDVCKGDI VFNIYHSKRS PQPPKKDSLG AHSITSPGGN NVQLIDKVWQ
LGRDYSMVES PLICKEGESV QGSHVTRWPG FYILQWKFHT MPACAATNLP RVDDVLASLQ
VSSHKCKVMY YTEVIGSEDF RGSMTSLESS HSGFSQLSAA TTSSSQSQSS SMISR
