S17AA_XENLA
ID S17AA_XENLA Reviewed; 380 AA.
AC Q3KQ35; O42600; Q90ZI0;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Transcription factor Sox-17-alpha-A;
DE Short=Xsox17alpha {ECO:0000312|EMBL:AAI06404.1};
DE Short=xSox17alpha1 {ECO:0000312|EMBL:BAB60827.1};
GN Name=sox17a-a; Synonyms=sox17a, sox17a1 {ECO:0000303|PubMed:12062811};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:CAA04957.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION.
RC TISSUE=Vegetal pole {ECO:0000269|PubMed:9363948};
RX PubMed=9363948; DOI=10.1016/s0092-8674(00)80423-7;
RA Hudson C., Clements D., Friday R.V., Stott D., Woodland H.R.;
RT "Xsox17alpha and -beta mediate endoderm formation in Xenopus.";
RL Cell 91:397-405(1997).
RN [2] {ECO:0000312|EMBL:AAI06404.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo {ECO:0000312|EMBL:AAI06404.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305, ECO:0000312|EMBL:BAB60827.1}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 27-380, AND TISSUE SPECIFICITY.
RX PubMed=12062811; DOI=10.1016/s0378-1119(02)00554-1;
RA Hasegawa M., Hiraoka Y., Hagiuda J., Ogawa M., Aiso S.;
RT "Expression and characterization of Xenopus laevis SRY-related cDNAs,
RT xSox17alpha1, xSox17alpha2, xSox18alpha and xSox18beta.";
RL Gene 290:163-172(2002).
RN [4] {ECO:0000305}
RP FUNCTION, AND INTERACTION WITH CTNNB1.
RX PubMed=10549281; DOI=10.1016/s1097-2765(00)80200-2;
RA Zorn A.M., Barish G.D., Williams B.O., Lavender P., Klymkowsky M.W.,
RA Varmus H.E.;
RT "Regulation of Wnt signaling by Sox proteins: XSox17 alpha/beta and XSox3
RT physically interact with beta-catenin.";
RL Mol. Cell 4:487-498(1999).
RN [5] {ECO:0000305}
RP FUNCTION.
RX PubMed=11091074; DOI=10.1016/s0925-4773(00)00476-7;
RA Clements D., Woodland H.R.;
RT "Changes in embryonic cell fate produced by expression of an endodermal
RT transcription factor, Xsox17.";
RL Mech. Dev. 99:65-70(2000).
RN [6] {ECO:0000305}
RP FUNCTION, AND INDUCTION.
RX PubMed=11124113; DOI=10.1242/dev.128.2.167;
RA Xanthos J.B., Kofron M., Wylie C., Heasman J.;
RT "Maternal VegT is the initiator of a molecular network specifying endoderm
RT in Xenopus laevis.";
RL Development 128:167-180(2001).
RN [7] {ECO:0000305}
RP FUNCTION, AND INDUCTION.
RX PubMed=11518513; DOI=10.1006/dbio.2001.0366;
RA Engleka M.J., Craig E.J., Kessler D.S.;
RT "VegT activation of Sox17 at the midblastula transition alters the response
RT to nodal signals in the vegetal endoderm domain.";
RL Dev. Biol. 237:159-172(2001).
RN [8] {ECO:0000305}
RP TISSUE SPECIFICITY.
RX PubMed=11335125; DOI=10.1016/s0925-4773(01)00341-0;
RA Zorn A.M., Mason J.;
RT "Gene expression in the embryonic Xenopus liver.";
RL Mech. Dev. 103:153-157(2001).
RN [9] {ECO:0000305}
RP FUNCTION, AND INDUCTION.
RX PubMed=12798301; DOI=10.1016/s0012-1606(03)00124-6;
RA Clements D., Woodland H.R.;
RT "VegT induces endoderm by a self-limiting mechanism and by changing the
RT competence of cells to respond to TGF-beta signals.";
RL Dev. Biol. 258:454-463(2003).
RN [10] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12591603; DOI=10.1016/s0925-4773(02)00450-1;
RA Clements D., Cameleyre I., Woodland H.R.;
RT "Redundant early and overlapping larval roles of Xsox17 subgroup genes in
RT Xenopus endoderm development.";
RL Mech. Dev. 120:337-348(2003).
RN [11] {ECO:0000305}
RP FUNCTION, INTERACTION WITH CTNNB1, AND MUTAGENESIS OF 329-GLU--TYR-333 AND
RP 331-GLU--TYR-333.
RX PubMed=15163629; DOI=10.1242/dev.01176;
RA Sinner D., Rankin S., Lee M., Zorn A.M.;
RT "Sox17 and beta-catenin cooperate to regulate the transcription of
RT endodermal genes.";
RL Development 131:3069-3080(2004).
RN [12] {ECO:0000305}
RP FUNCTION.
RX PubMed=15157240; DOI=10.1111/j.1432-0436.2004.07204005.x;
RA Ahmed N., Howard L., Woodland H.R.;
RT "Early endodermal expression of the Xenopus Endodermin gene is driven by
RT regulatory sequences containing essential Sox protein-binding elements.";
RL Differentiation 72:171-184(2004).
RN [13] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=16651540; DOI=10.1242/dev.02358;
RA Sinner D., Kirilenko P., Rankin S., Wei E., Howard L., Kofron M.,
RA Heasman J., Woodland H.R., Zorn A.M.;
RT "Global analysis of the transcriptional network controlling Xenopus
RT endoderm formation.";
RL Development 133:1955-1966(2006).
RN [14] {ECO:0000305}
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=17719026; DOI=10.1016/j.ydbio.2007.07.028;
RA Howard L., Rex M., Clements D., Woodland H.R.;
RT "Regulation of the Xenopus Xsox17alpha(1) promoter by co-operating VegT and
RT Sox17 sites.";
RL Dev. Biol. 310:402-415(2007).
RN [15] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=19003160; DOI=10.1007/s10616-008-9127-2;
RA Yagi Y., Ito Y., Kuhara S., Tashiro K.;
RT "Cephalic hedgehog expression is regulated directly by Sox17 in endoderm
RT development of Xenopus laevis.";
RL Cytotechnology 57:151-159(2008).
CC -!- FUNCTION: Transcriptional activator. Binds to the DNA sequence 5'-
CC AACAAT-3' (By similarity). All of the sox17 proteins are required for
CC embryonic endoderm development and gastrulation movements, and show
CC some redundancy in function. In addition, the sox17 proteins have
CC distinct but overlapping roles in later gut development. Acts
CC downstream of vegt-signaling in endoderm differentiation to induce a
CC range of endodermal genes both directly (including endodermin and
CC dhh/chh) and indirectly. Also represses wnt-responsive genes to inhibit
CC wnt/beta-catenin signaling. {ECO:0000250, ECO:0000269|PubMed:10549281,
CC ECO:0000269|PubMed:11091074, ECO:0000269|PubMed:11124113,
CC ECO:0000269|PubMed:11518513, ECO:0000269|PubMed:12591603,
CC ECO:0000269|PubMed:12798301, ECO:0000269|PubMed:15157240,
CC ECO:0000269|PubMed:15163629, ECO:0000269|PubMed:16651540,
CC ECO:0000269|PubMed:19003160, ECO:0000269|PubMed:9363948}.
CC -!- SUBUNIT: Interacts (via C-terminus) with ctnnb1/beta-catenin (via
CC Armadillo repeats); this interaction is required for inhibition of wnt-
CC signaling. {ECO:0000269|PubMed:10549281, ECO:0000269|PubMed:15163629}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00267,
CC ECO:0000269|PubMed:9363948}.
CC -!- TISSUE SPECIFICITY: In early gastrulae, expressed in the vegetal but
CC not animal hemisphere. The vegetal region is fated to become endoderm,
CC and endodermal expression continues throughout gastrulation and
CC neurulation. At tailbud stages, expression is down-regulated and
CC becomes restricted to the most posterior endoderm and the future
CC liver/gall bladder region. By 3-7 days, endodermal expression is
CC restricted to the gall bladder bud. Also expressed in the embryonic
CC gut, with strong expression in the posterior gut during tailbud stages,
CC but by stage 40, expression rises again in the anterior gut. Expressed
CC at a low level in the adult kidney and spleen.
CC {ECO:0000269|PubMed:11335125, ECO:0000269|PubMed:12062811,
CC ECO:0000269|PubMed:12591603, ECO:0000269|PubMed:16651540,
CC ECO:0000269|PubMed:17719026, ECO:0000269|PubMed:19003160,
CC ECO:0000269|PubMed:9363948}.
CC -!- DEVELOPMENTAL STAGE: Expressed zygotically from late blastula stage.
CC Expressed at all subsequent stages from 10 (early gastrula) to 35
CC (early tadpole). {ECO:0000269|PubMed:9363948}.
CC -!- INDUCTION: Involved in multiple regulatory feedback loops with other
CC endodermal factors, including the nodal-related factors/Xnrs.
CC Autoinduces. Induced by activin. Induced directly by vegt; originally
CC this is in a cell-autonomous fashion but subsequently cell contact is
CC required for expression to be maintained. {ECO:0000269|PubMed:11124113,
CC ECO:0000269|PubMed:11518513, ECO:0000269|PubMed:12798301,
CC ECO:0000269|PubMed:16651540, ECO:0000269|PubMed:17719026,
CC ECO:0000269|PubMed:9363948}.
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors.
CC {ECO:0000250|UniProtKB:Q9H6I2}.
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DR EMBL; AJ001730; CAA04957.1; -; mRNA.
DR EMBL; BC106403; AAI06404.1; -; mRNA.
DR EMBL; AB052690; BAB60827.1; -; mRNA.
DR RefSeq; NP_001081631.1; NM_001088162.1.
DR AlphaFoldDB; Q3KQ35; -.
DR SMR; Q3KQ35; -.
DR MaxQB; Q3KQ35; -.
DR DNASU; 397966; -.
DR GeneID; 397966; -.
DR KEGG; xla:397966; -.
DR CTD; 397966; -.
DR Xenbase; XB-GENE-865067; sox17a.L.
DR OrthoDB; 1042753at2759; -.
DR Proteomes; UP000186698; Chromosome 6L.
DR Bgee; 397966; Expressed in gastrula and 15 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0008013; F:beta-catenin binding; IDA:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0042074; P:cell migration involved in gastrulation; IMP:UniProtKB.
DR GO; GO:0007492; P:endoderm development; IMP:UniProtKB.
DR GO; GO:0007440; P:foregut morphogenesis; IMP:UniProtKB.
DR GO; GO:0007442; P:hindgut morphogenesis; IMP:UniProtKB.
DR GO; GO:0007494; P:midgut development; IMP:UniProtKB.
DR GO; GO:0090090; P:negative regulation of canonical Wnt signaling pathway; IDA:UniProtKB.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IDA:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR Gene3D; 1.10.30.10; -; 1.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR InterPro; IPR033392; Sox7/17/18_central.
DR InterPro; IPR021934; Sox_C.
DR Pfam; PF00505; HMG_box; 1.
DR Pfam; PF12067; Sox17_18_mid; 1.
DR SMART; SM00398; HMG; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
DR PROSITE; PS50118; HMG_BOX_2; 1.
DR PROSITE; PS51516; SOX_C; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; DNA-binding; Gastrulation; Nucleus;
KW Reference proteome; Transcription; Transcription regulation;
KW Wnt signaling pathway.
FT CHAIN 1..380
FT /note="Transcription factor Sox-17-alpha-A"
FT /id="PRO_0000376050"
FT DOMAIN 264..379
FT /note="Sox C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00849"
FT DNA_BIND 61..129
FT /note="HMG box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 40..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 292..312
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 329..334
FT /note="Required for transcriptional activity and
FT interaction with ctnnb1"
FT /evidence="ECO:0000269|PubMed:15163629"
FT MOTIF 328..336
FT /note="9aaTAD"
FT /evidence="ECO:0000250|UniProtKB:Q9H6I2"
FT MUTAGEN 329..333
FT /note="Missing: Significantly reduces transcriptional
FT activation activity. Impairs interaction with ctnnb1."
FT /evidence="ECO:0000269|PubMed:15163629"
FT MUTAGEN 331..333
FT /note="EQY->GGG: Significantly reduces transcriptional
FT activation activity. Impairs interaction with ctnnb1."
FT /evidence="ECO:0000269|PubMed:15163629"
FT CONFLICT 181
FT /note="P -> S (in Ref. 1; CAA04957)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 380 AA; 42656 MW; BBB4D116897C69AA CRC64;
MSSPDGGYAS DDQNQGKCSV PIMMTGLGQC QWAEPMTSLG EGKLKSDAGS ANSRSKAEAR
IRRPMNAFMV WAKDERKRLA QQNPDLHNAE LSKMLGKSWK SLTLAEKRPF VEEAERLRVQ
HMQDHPNYKY RPRRRKQVKR MKRAENGFMH MAEAQESAVL GADGRMCLEN FSLGYHEQTY
PHGQVPQSSH YREPQAVAPH YDGYSLPTPE SSPLDLAEAD PVFFTSPAQD ECQMMPYSYN
GSYPHQQNSM LVRQMPQTEQ MGQVSPVQGM MACQSSPHMY YGQMYLPGSA RHHQLHQAGQ
PSPPPEAQQM GRADHIQPAD MLAEVDRTEF EQYLSYVAKS DLGMHYHAQE SVVPTADNGP
ISSVLSDAST AVYYCNYPSA
