S17AB_XENLA
ID S17AB_XENLA Reviewed; 377 AA.
AC Q90ZH9; Q6PAE5;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Transcription factor Sox-17-alpha-B;
DE Short=xSox17alpha2 {ECO:0000312|EMBL:BAB60828.1};
GN Name=sox17a-b; Synonyms=sox17a2 {ECO:0000303|PubMed:12062811};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:BAB60828.1}
RP NUCLEOTIDE SEQUENCE [MRNA], DNA-BINDING, AND TISSUE SPECIFICITY.
RX PubMed=12062811; DOI=10.1016/s0378-1119(02)00554-1;
RA Hasegawa M., Hiraoka Y., Hagiuda J., Ogawa M., Aiso S.;
RT "Expression and characterization of Xenopus laevis SRY-related cDNAs,
RT xSox17alpha1, xSox17alpha2, xSox18alpha and xSox18beta.";
RL Gene 290:163-172(2002).
RN [2] {ECO:0000312|EMBL:AAH60350.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo {ECO:0000312|EMBL:AAH60350.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12591603; DOI=10.1016/s0925-4773(02)00450-1;
RA Clements D., Cameleyre I., Woodland H.R.;
RT "Redundant early and overlapping larval roles of Xsox17 subgroup genes in
RT Xenopus endoderm development.";
RL Mech. Dev. 120:337-348(2003).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=15302595; DOI=10.1016/j.ydbio.2004.05.019;
RA Zhang C., Basta T., Hernandez-Lagunas L., Simpson P., Stemple D.L.,
RA Artinger K.B., Klymkowsky M.W.;
RT "Repression of nodal expression by maternal B1-type SOXs regulates germ
RT layer formation in Xenopus and zebrafish.";
RL Dev. Biol. 273:23-37(2004).
RN [5] {ECO:0000305}
RP FUNCTION.
RX PubMed=15157240; DOI=10.1111/j.1432-0436.2004.07204005.x;
RA Ahmed N., Howard L., Woodland H.R.;
RT "Early endodermal expression of the Xenopus Endodermin gene is driven by
RT regulatory sequences containing essential Sox protein-binding elements.";
RL Differentiation 72:171-184(2004).
RN [6] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=16651540; DOI=10.1242/dev.02358;
RA Sinner D., Kirilenko P., Rankin S., Wei E., Howard L., Kofron M.,
RA Heasman J., Woodland H.R., Zorn A.M.;
RT "Global analysis of the transcriptional network controlling Xenopus
RT endoderm formation.";
RL Development 133:1955-1966(2006).
CC -!- FUNCTION: Transcription activator. Binds to the DNA sequence 5'-AACAAT-
CC 3'. All of the sox17 proteins are required for embryonic endoderm
CC development and gastrulation movements, and show some redundancy in
CC function. In addition, the sox17 proteins have distinct but overlapping
CC roles in later gut development. Acts downstream of vegt-signaling in
CC endoderm differentiation to induce a range of endodermal genes both
CC directly (including endodermin and dhh/chh) and indirectly. Also
CC represses wnt-responsive genes to inhibit wnt/beta-catenin signaling.
CC {ECO:0000250|UniProtKB:Q3KQ35, ECO:0000269|PubMed:12062811,
CC ECO:0000269|PubMed:12591603, ECO:0000269|PubMed:15157240,
CC ECO:0000269|PubMed:15302595, ECO:0000269|PubMed:16651540}.
CC -!- SUBUNIT: Interacts (via C-terminus) with ctnnb1/beta-catenin (via
CC Armadillo repeats); this interaction is required for inhibition of wnt-
CC signaling. {ECO:0000250|UniProtKB:Q3KQ35}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q3KQ35,
CC ECO:0000255|PROSITE-ProRule:PRU00267}.
CC -!- TISSUE SPECIFICITY: Enriched in the embryonic endoderm. Expressed in
CC the embryonic gut, with strong expression in the posterior gut during
CC tailbud stages. Expressed at a low level in the adult kidney and
CC spleen. {ECO:0000269|PubMed:12062811, ECO:0000269|PubMed:12591603,
CC ECO:0000269|PubMed:16651540}.
CC -!- INDUCTION: Involved in multiple regulatory feedback loops with other
CC endodermal factors, including the nodal-related factors/Xnrs.
CC Autoinduces. {ECO:0000269|PubMed:16651540}.
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors.
CC {ECO:0000250|UniProtKB:Q9H6I2}.
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DR EMBL; AB052691; BAB60828.1; -; mRNA.
DR EMBL; BC060350; AAH60350.1; -; mRNA.
DR RefSeq; NP_001083136.1; NM_001089667.1.
DR AlphaFoldDB; Q90ZH9; -.
DR SMR; Q90ZH9; -.
DR DNASU; 398765; -.
DR GeneID; 398765; -.
DR KEGG; xla:398765; -.
DR CTD; 398765; -.
DR Xenbase; XB-GENE-6255435; sox17a.S.
DR OMA; CDAQGLF; -.
DR OrthoDB; 1042753at2759; -.
DR Proteomes; UP000186698; Chromosome 6S.
DR Bgee; 398765; Expressed in gastrula and 18 other tissues.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0008013; F:beta-catenin binding; ISS:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0042074; P:cell migration involved in gastrulation; IMP:UniProtKB.
DR GO; GO:0007492; P:endoderm development; IMP:UniProtKB.
DR GO; GO:0007442; P:hindgut morphogenesis; IMP:UniProtKB.
DR GO; GO:0007494; P:midgut development; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR Gene3D; 1.10.30.10; -; 1.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR InterPro; IPR033392; Sox7/17/18_central.
DR InterPro; IPR021934; Sox_C.
DR Pfam; PF00505; HMG_box; 1.
DR Pfam; PF12067; Sox17_18_mid; 1.
DR SMART; SM00398; HMG; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
DR PROSITE; PS50118; HMG_BOX_2; 1.
DR PROSITE; PS51516; SOX_C; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; DNA-binding; Gastrulation; Nucleus;
KW Reference proteome; Transcription; Transcription regulation;
KW Wnt signaling pathway.
FT CHAIN 1..377
FT /note="Transcription factor Sox-17-alpha-B"
FT /id="PRO_0000376051"
FT DOMAIN 262..376
FT /note="Sox C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00849"
FT DNA_BIND 58..126
FT /note="HMG box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 31..57
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 282..321
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 326..331
FT /note="Required for transcriptional activity and
FT interaction with ctnnb1"
FT /evidence="ECO:0000250|UniProtKB:Q3KQ35"
FT MOTIF 325..333
FT /note="9aaTAD"
FT /evidence="ECO:0000250|UniProtKB:Q9H6I2"
FT CONFLICT 33
FT /note="S -> A (in Ref. 2; AAH60350)"
FT /evidence="ECO:0000305"
FT CONFLICT 50
FT /note="S -> Y (in Ref. 2; AAH60350)"
FT /evidence="ECO:0000305"
FT CONFLICT 56
FT /note="G -> T (in Ref. 2; AAH60350)"
FT /evidence="ECO:0000305"
FT CONFLICT 159
FT /note="T -> A (in Ref. 2; AAH60350)"
FT /evidence="ECO:0000305"
FT CONFLICT 261
FT /note="E -> Q (in Ref. 2; AAH60350)"
FT /evidence="ECO:0000305"
FT CONFLICT 342
FT /note="N -> H (in Ref. 2; AAH60350)"
FT /evidence="ECO:0000305"
FT CONFLICT 350
FT /note="G -> V (in Ref. 2; AAH60350)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 377 AA; 42385 MW; 7C3762AD3439DCDB CRC64;
MSSPDGGYGS DDQNQGKCSV PIMMSGLGQC QWSEPMTSLG EGKLKSDANS RSKAEGRIRR
PMNAFMVWAK DERKRLAQQN PDLHNAELSK MLGKSWKALS LAEKRPFVEE AERLRVQHMQ
DHPNYKYRPR RRKQVKRMKR AENGFMHMTE AQESAVMGTD GRMCLENFNL GFHEQTYPQL
PQASHYREPQ AMAPHYDGYS LPTPESSPLD LAEADPVFFT SPAQDECQMM PYSYNSSYTH
QHNSGASMLV RQMPQTEQIG EGSPVEGMMA CQSSPHMYYG QMYLPGSTRH HQHPQAGQPS
PPPEAQQLGR ADQTQQADMM AEDRTEFEQY LSYVSKSDLG MNYHGQESVG PTADNGPISS
VLSDATTAVY YCNYPSA
