S17B1_XENLA
ID S17B1_XENLA Reviewed; 373 AA.
AC O42601; Q6NRU8;
DT 01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Transcription factor Sox-17-beta.1 {ECO:0000250|UniProtKB:Q9H6I2};
DE AltName: Full=SRY (sex determining region Y)-box 17-beta.1;
DE AltName: Full=Transcription factor Sox-17-beta {ECO:0000303|PubMed:9363948};
DE Short=XSox17beta {ECO:0000303|PubMed:9363948};
GN Name=sox17b.1; Synonyms=sox17b {ECO:0000303|PubMed:9363948};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:CAA04967.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION.
RC TISSUE=Vegetal pole {ECO:0000269|PubMed:9363948};
RX PubMed=9363948; DOI=10.1016/s0092-8674(00)80423-7;
RA Hudson C., Clements D., Friday R.V., Stott D., Woodland H.R.;
RT "Xsox17alpha and -beta mediate endoderm formation in Xenopus.";
RL Cell 91:397-405(1997).
RN [2] {ECO:0000312|EMBL:AAH70615.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Gastrula {ECO:0000312|EMBL:AAH70615.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP FUNCTION, DNA-BINDING, AND INTERACTION WITH CTNNB1.
RX PubMed=10549281; DOI=10.1016/s1097-2765(00)80200-2;
RA Zorn A.M., Barish G.D., Williams B.O., Lavender P., Klymkowsky M.W.,
RA Varmus H.E.;
RT "Regulation of Wnt signaling by Sox proteins: XSox17 alpha/beta and XSox3
RT physically interact with beta-catenin.";
RL Mol. Cell 4:487-498(1999).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=11091074; DOI=10.1016/s0925-4773(00)00476-7;
RA Clements D., Woodland H.R.;
RT "Changes in embryonic cell fate produced by expression of an endodermal
RT transcription factor, Xsox17.";
RL Mech. Dev. 99:65-70(2000).
RN [5] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12591603; DOI=10.1016/s0925-4773(02)00450-1;
RA Clements D., Cameleyre I., Woodland H.R.;
RT "Redundant early and overlapping larval roles of Xsox17 subgroup genes in
RT Xenopus endoderm development.";
RL Mech. Dev. 120:337-348(2003).
RN [6] {ECO:0000305}
RP INDUCTION.
RX PubMed=12798301; DOI=10.1016/s0012-1606(03)00124-6;
RA Clements D., Woodland H.R.;
RT "VegT induces endoderm by a self-limiting mechanism and by changing the
RT competence of cells to respond to TGF-beta signals.";
RL Dev. Biol. 258:454-463(2003).
RN [7] {ECO:0000305}
RP FUNCTION, INTERACTION WITH CTNNB1, AND MUTAGENESIS OF GLY-93;
RP 324-GLU--TYR-328 AND 326-ASP--TYR-328.
RX PubMed=15163629; DOI=10.1242/dev.01176;
RA Sinner D., Rankin S., Lee M., Zorn A.M.;
RT "Sox17 and beta-catenin cooperate to regulate the transcription of
RT endodermal genes.";
RL Development 131:3069-3080(2004).
RN [8] {ECO:0000305}
RP FUNCTION.
RX PubMed=15302595; DOI=10.1016/j.ydbio.2004.05.019;
RA Zhang C., Basta T., Hernandez-Lagunas L., Simpson P., Stemple D.L.,
RA Artinger K.B., Klymkowsky M.W.;
RT "Repression of nodal expression by maternal B1-type SOXs regulates germ
RT layer formation in Xenopus and zebrafish.";
RL Dev. Biol. 273:23-37(2004).
RN [9] {ECO:0000305}
RP FUNCTION.
RX PubMed=15157240; DOI=10.1111/j.1432-0436.2004.07204005.x;
RA Ahmed N., Howard L., Woodland H.R.;
RT "Early endodermal expression of the Xenopus Endodermin gene is driven by
RT regulatory sequences containing essential Sox protein-binding elements.";
RL Differentiation 72:171-184(2004).
RN [10] {ECO:0000305}
RP INDUCTION.
RX PubMed=15680368; DOI=10.1016/j.ydbio.2004.11.008;
RA Zhang C., Basta T., Fawcett S.R., Klymkowsky M.W.;
RT "SOX7 is an immediate-early target of VegT and regulates Nodal-related gene
RT expression in Xenopus.";
RL Dev. Biol. 278:526-541(2005).
RN [11] {ECO:0000305}
RP FUNCTION.
RX PubMed=16193513; DOI=10.1002/dvdy.20565;
RA Zhang C., Basta T., Klymkowsky M.W.;
RT "SOX7 and SOX18 are essential for cardiogenesis in Xenopus.";
RL Dev. Dyn. 234:878-891(2005).
RN [12] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=16651540; DOI=10.1242/dev.02358;
RA Sinner D., Kirilenko P., Rankin S., Wei E., Howard L., Kofron M.,
RA Heasman J., Woodland H.R., Zorn A.M.;
RT "Global analysis of the transcriptional network controlling Xenopus
RT endoderm formation.";
RL Development 133:1955-1966(2006).
RN [13] {ECO:0000305}
RP FUNCTION.
RX PubMed=16278889; DOI=10.1002/dvdy.20636;
RA Dickinson K., Leonard J., Baker J.C.;
RT "Genomic profiling of mixer and Sox17beta targets during Xenopus endoderm
RT development.";
RL Dev. Dyn. 235:368-381(2006).
RN [14] {ECO:0000305}
RP FUNCTION, AND DNA-BINDING.
RX PubMed=17719026; DOI=10.1016/j.ydbio.2007.07.028;
RA Howard L., Rex M., Clements D., Woodland H.R.;
RT "Regulation of the Xenopus Xsox17alpha(1) promoter by co-operating VegT and
RT Sox17 sites.";
RL Dev. Biol. 310:402-415(2007).
RN [15] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=19003160; DOI=10.1007/s10616-008-9127-2;
RA Yagi Y., Ito Y., Kuhara S., Tashiro K.;
RT "Cephalic hedgehog expression is regulated directly by Sox17 in endoderm
RT development of Xenopus laevis.";
RL Cytotechnology 57:151-159(2008).
RN [16] {ECO:0000305}
RP FUNCTION, AND INDUCTION.
RX PubMed=18651654; DOI=10.1002/dvdy.21631;
RA Wills A., Dickinson K., Khokha M., Baker J.C.;
RT "Bmp signaling is necessary and sufficient for ventrolateral endoderm
RT specification in Xenopus.";
RL Dev. Dyn. 237:2177-2186(2008).
CC -!- FUNCTION: Transcription activator. Doesn't appear to bind to the
CC consensus 5'-AACAAT-3' DNA binding site, but binds 5'-ATTGTT-3'. All of
CC the sox17 proteins are required for embryonic endoderm development and
CC gastrulation movements, and show some redundancy in function. In
CC addition, the sox17 proteins have distinct but overlapping roles in
CC later gut development. Acts downstream of vegt-signaling in endoderm
CC differentiation to induce a range of endodermal genes both directly and
CC indirectly. Also represses wnt-responsive genes to inhibit wnt/beta-
CC catenin-mediated signaling. {ECO:0000269|PubMed:10549281,
CC ECO:0000269|PubMed:11091074, ECO:0000269|PubMed:12591603,
CC ECO:0000269|PubMed:15157240, ECO:0000269|PubMed:15163629,
CC ECO:0000269|PubMed:15302595, ECO:0000269|PubMed:16193513,
CC ECO:0000269|PubMed:16278889, ECO:0000269|PubMed:16651540,
CC ECO:0000269|PubMed:17719026, ECO:0000269|PubMed:18651654,
CC ECO:0000269|PubMed:19003160, ECO:0000269|PubMed:9363948}.
CC -!- SUBUNIT: Interacts (via C-terminus) with ctnnb1/beta-catenin (via
CC Armadillo repeats); this interaction is required for inhibition of wnt-
CC signaling. {ECO:0000269|PubMed:10549281, ECO:0000269|PubMed:15163629}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00267,
CC ECO:0000269|PubMed:9363948}.
CC -!- TISSUE SPECIFICITY: In early gastrulae, expressed in the vegetal but
CC not animal hemisphere. The vegetal region is fated to become endoderm
CC and endodermal expression continues throughout gastrulation and
CC neurulation. At tailbud stages, expression is barely detectable.
CC {ECO:0000269|PubMed:12591603, ECO:0000269|PubMed:16651540,
CC ECO:0000269|PubMed:19003160, ECO:0000269|PubMed:9363948}.
CC -!- DEVELOPMENTAL STAGE: Expressed zygotically from late blastula stage.
CC Expression is barely detectable during tailbud stages and is absent by
CC stage 35 (early tadpole). {ECO:0000269|PubMed:9363948}.
CC -!- INDUCTION: Involved in multiple regulatory feedback loops with other
CC endodermal factors, including the nodal-related factors/Xnrs.
CC Autoinduces. By activin. Directly by vegt; originally cell-autonomously
CC and then cell contact is required for expression to be maintained. By
CC bmp-signaling. {ECO:0000269|PubMed:12798301,
CC ECO:0000269|PubMed:15680368, ECO:0000269|PubMed:16651540,
CC ECO:0000269|PubMed:18651654, ECO:0000269|PubMed:9363948}.
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors.
CC {ECO:0000250|UniProtKB:Q9H6I2}.
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DR EMBL; AJ001742; CAA04967.1; -; mRNA.
DR EMBL; BC070615; AAH70615.1; -; mRNA.
DR RefSeq; NP_001081633.1; NM_001088164.1.
DR AlphaFoldDB; O42601; -.
DR PRIDE; O42601; -.
DR DNASU; 397967; -.
DR GeneID; 397967; -.
DR KEGG; xla:397967; -.
DR CTD; 397967; -.
DR Xenbase; XB-GENE-6252148; sox17b.1.S.
DR OrthoDB; 1641977at2759; -.
DR Proteomes; UP000186698; Chromosome 6S.
DR Bgee; 397967; Expressed in gastrula and 4 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0008013; F:beta-catenin binding; IDA:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0007492; P:endoderm development; IMP:UniProtKB.
DR GO; GO:0007369; P:gastrulation; IEA:UniProtKB-KW.
DR GO; GO:0007494; P:midgut development; IMP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0030178; P:negative regulation of Wnt signaling pathway; IGI:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR Gene3D; 1.10.30.10; -; 1.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR InterPro; IPR021934; Sox_C.
DR Pfam; PF00505; HMG_box; 1.
DR SMART; SM00398; HMG; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
DR PROSITE; PS50118; HMG_BOX_2; 1.
DR PROSITE; PS51516; SOX_C; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; DNA-binding; Gastrulation; Nucleus;
KW Reference proteome; Transcription; Transcription regulation;
KW Wnt signaling pathway.
FT CHAIN 1..373
FT /note="Transcription factor Sox-17-beta.1"
FT /id="PRO_0000382472"
FT DOMAIN 256..373
FT /note="Sox C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00849"
FT DNA_BIND 58..126
FT /note="HMG box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 324..328
FT /note="Required for transcriptional activity and
FT interaction with ctnnb1"
FT /evidence="ECO:0000269|PubMed:15163629"
FT MOTIF 323..331
FT /note="9aaTAD"
FT /evidence="ECO:0000250|UniProtKB:Q9H6I2"
FT MUTAGEN 93
FT /note="G->R: No effect on ctnnb1-binding."
FT /evidence="ECO:0000269|PubMed:15163629"
FT MUTAGEN 324..328
FT /note="Missing: Significantly reduces transcriptional
FT activation activity. Impairs interaction with ctnnb1."
FT /evidence="ECO:0000269|PubMed:15163629"
FT MUTAGEN 326..328
FT /note="DQY->GGG: Significantly reduces transcriptional
FT activation activity. Impairs interaction with ctnnb1."
FT /evidence="ECO:0000269|PubMed:15163629"
FT CONFLICT 193
FT /note="M -> I (in Ref. 2; AAH70615)"
FT /evidence="ECO:0000305"
FT CONFLICT 302
FT /note="T -> M (in Ref. 2; AAH70615)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 373 AA; 43248 MW; 425711BA12D657AD CRC64;
MSSPDCGYSS DDQIQGSCSV PMMMGQYQWT EPLTVFQDLK PKRDEGSADS RSKAEGRIRR
PMNAFMVWAK DERKRLAQQN PDLHNAELSK MLGKSWKSLT LATKRPFVEE AERLRVQHIQ
DYPDYKYRPR RKKQVKRMKR EEDGFLPSAN FPGSQIMDNN VMVGENYRMQ YSAQNHQQNQ
LPPAGYFEGH NSMGYYYRDY SVPNYHISQN SSGYDSPPAQ DEYQALSYSF NSSYMPYQQN
ATTPVMAKQM AVTQNIPQES PEHGMMASPQ MYNRQMYVSE CAKTHPMAQT EQHFPSYQSQ
KTVRQNYLQS QQDGHLESDI DKTEFDQYLM YEPKADMEII YTIDQDSGAY STNLLPSLIT
EANNVCYYDY CGV
