S17P_SPIOL
ID S17P_SPIOL Reviewed; 387 AA.
AC O20252;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Sedoheptulose-1,7-bisphosphatase, chloroplastic;
DE EC=3.1.3.37;
DE AltName: Full=SED(1,7)P2ase;
DE AltName: Full=Sedoheptulose bisphosphatase;
DE Short=SBPase;
DE Flags: Precursor;
OS Spinacia oleracea (Spinach).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Seedling;
RX PubMed=8980497; DOI=10.1007/bf00019100;
RA Martin W., Mustafa A.Z., Henze K., Schnarrenberger C.;
RT "Higher-plant chloroplast and cytosolic fructose-1,6-bisphosphatase
RT isoenzymes: origins via duplication rather than prokaryote-eukaryote
RT divergence.";
RL Plant Mol. Biol. 32:485-491(1996).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-sedoheptulose 1,7-bisphosphate + H2O = D-sedoheptulose 7-
CC phosphate + phosphate; Xref=Rhea:RHEA:17461, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:57483, ChEBI:CHEBI:58335; EC=3.1.3.37;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305};
CC Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000305};
CC -!- PATHWAY: Carbohydrate biosynthesis; Calvin cycle.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the FBPase class 1 family. {ECO:0000305}.
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DR EMBL; L76556; AAB81104.1; -; mRNA.
DR PIR; T09086; T09086.
DR AlphaFoldDB; O20252; -.
DR SMR; O20252; -.
DR IntAct; O20252; 1.
DR PRIDE; O20252; -.
DR OrthoDB; 1381522at2759; -.
DR SABIO-RK; O20252; -.
DR UniPathway; UPA00116; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0050278; F:sedoheptulose-bisphosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0019253; P:reductive pentose-phosphate cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd00354; FBPase; 1.
DR HAMAP; MF_01855; FBPase_class1; 1.
DR InterPro; IPR044015; FBPase_C_dom.
DR InterPro; IPR000146; FBPase_class-1.
DR InterPro; IPR033391; FBPase_N.
DR InterPro; IPR020548; Fructose_bisphosphatase_AS.
DR InterPro; IPR023079; SBPase.
DR PANTHER; PTHR11556; PTHR11556; 1.
DR Pfam; PF00316; FBPase; 1.
DR Pfam; PF18913; FBPase_C; 1.
DR PRINTS; PR01958; S17BPHPHTASE.
DR PROSITE; PS00124; FBPASE; 1.
PE 2: Evidence at transcript level;
KW Calvin cycle; Carbohydrate metabolism; Chloroplast; Disulfide bond;
KW Hydrolase; Magnesium; Metal-binding; Plastid; Transit peptide.
FT TRANSIT 1..?
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN ?..387
FT /note="Sedoheptulose-1,7-bisphosphatase, chloroplastic"
FT /id="PRO_0000008823"
FT BINDING 120
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000255"
FT BINDING 149
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000255"
FT BINDING 149
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000255"
FT BINDING 170
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000255"
FT BINDING 170
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000255"
FT BINDING 172
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000255"
FT BINDING 173..176
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 173
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000255"
FT BINDING 284
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 314
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 320
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000255"
FT DISULFID 109..114
FT /note="Redox-active (light-modulated)"
FT /evidence="ECO:0000255"
SQ SEQUENCE 387 AA; 42081 MW; 746AE554C6844B79 CRC64;
METSMACCSR SIVLPRVSPQ HSSALVPSSI NLKSLKSSSL FGESLRMTTK SSVRVNKAKN
SSLVTKCELG DSLEEFLAKA TTDKGLIRLM MCMGEALRTI GFKVRTASCG GTQCVNTFGD
EQLAIDVLAD KLLFEALNYS HFCKYACSEE LPELQDMGGP VDGGFSVAFD PLDGSSIVDT
NFSVGTIFGV WPGDKLTGVT GRDQVAAAMG IYGPRTTYVL ALKDYPGTHE FLLLDEGKWQ
HVKETTEINE GKLFCPGNLR ATSDNADYAK LIQYYIKEKY TLRYTGGMVP DVNQIIVKEK
GIFTNVISPT AKAKLRLLFE VAPLGFLIEK AGGHSSEGTK SVLDIEVKNL DDRTQVAYGS
LNEIIRFEKT LYGSSRLEEP VPVGAAA
