S1PR2_RAT
ID S1PR2_RAT Reviewed; 352 AA.
AC P47752; Q54AI6;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Sphingosine 1-phosphate receptor 2;
DE Short=S1P receptor 2;
DE Short=S1P2;
DE AltName: Full=AGR16;
DE AltName: Full=Endothelial differentiation G-protein coupled receptor 5;
DE AltName: Full=G-protein coupled receptor H218;
DE AltName: Full=Sphingosine 1-phosphate receptor Edg-5;
DE Short=S1P receptor Edg-5;
GN Name=S1pr2; Synonyms=Edg5;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Aortic smooth muscle;
RX PubMed=8382486; DOI=10.1006/bbrc.1993.1163;
RA Okazaki H., Ishizaka N., Sakurai T., Kurokawa K., Goto K., Kumada M.,
RA Takuwa Y.;
RT "Molecular cloning of a novel putative G protein-coupled receptor expressed
RT in the cardiovascular system.";
RL Biochem. Biophys. Res. Commun. 190:1104-1109(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain, and Lung;
RX PubMed=8087418; DOI=10.1006/mcne.1994.1024;
RA Maclennan A.J., Browe C.S., Gaskin A.A., Lado D.C., Shaw G.;
RT "Cloning and characterization of a putative G-protein coupled receptor
RT potentially involved in development.";
RL Mol. Cell. Neurosci. 5:201-209(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=9409733; DOI=10.1016/s0014-5793(97)01301-x;
RA An S., Bleu T., Huang W., Hallmark O.G., Coughlin S.R., Goetzl E.J.;
RT "Identification of cDNAs encoding two G protein-coupled receptors for
RT lysosphingolipids.";
RL FEBS Lett. 417:279-282(1997).
RN [4]
RP PHARMACOLOGICAL CHARACTERIZATION.
RX PubMed=10383399; DOI=10.1074/jbc.274.27.18997;
RA Ancellin N., Hla T.;
RT "Differential pharmacological properties and signal transduction of the
RT sphingosine 1-phosphate receptors EDG-1, EDG-3, and EDG-5.";
RL J. Biol. Chem. 274:18997-19002(1999).
RN [5]
RP FUNCTION.
RX PubMed=29453251; DOI=10.1161/circulationaha.117.032398;
RA Wang Y., Chen D., Zhang Y., Wang P., Zheng C., Zhang S., Yu B., Zhang L.,
RA Zhao G., Ma B., Cai Z., Xie N., Huang S., Liu Z., Mo X., Guan Y., Wang X.,
RA Fu Y., Ma D., Wang Y., Kong W.;
RT "Novel Adipokine, FAM19A5, Inhibits Neointima Formation After Injury
RT Through Sphingosine-1-Phosphate Receptor 2.";
RL Circulation 138:48-63(2018).
CC -!- FUNCTION: Receptor for the lysosphingolipid sphingosine 1-phosphate
CC (S1P) (PubMed:10383399). S1P is a bioactive lysophospholipid that
CC elicits diverse physiological effects on most types of cells and
CC tissues (PubMed:10383399). Receptor for the chemokine-like protein
CC FAM19A5 (PubMed:29453251). Mediates the inhibitory effect of FAM19A5 on
CC vascular smooth muscle cell proliferation and migration
CC (PubMed:29453251). {ECO:0000269|PubMed:10383399,
CC ECO:0000269|PubMed:29453251}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in all developing tissues with highest
CC levels detected in primitive, transformed cells. Relative abundance:
CC lung > kidney = skin = gut > spleen > brain > liver.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AB016931; BAA32454.1; -; mRNA.
DR EMBL; U10699; AAA19241.1; -; mRNA.
DR EMBL; AF022138; AAC53494.1; -; mRNA.
DR PIR; JC1465; JC1465.
DR RefSeq; NP_058888.1; NM_017192.1.
DR AlphaFoldDB; P47752; -.
DR SMR; P47752; -.
DR STRING; 10116.ENSRNOP00000028034; -.
DR BindingDB; P47752; -.
DR ChEMBL; CHEMBL3616360; -.
DR GuidetoPHARMACOLOGY; 276; -.
DR GlyGen; P47752; 1 site.
DR iPTMnet; P47752; -.
DR PhosphoSitePlus; P47752; -.
DR PaxDb; P47752; -.
DR Ensembl; ENSRNOT00000107018; ENSRNOP00000080559; ENSRNOG00000020653.
DR GeneID; 29415; -.
DR KEGG; rno:29415; -.
DR UCSC; RGD:68334; rat.
DR CTD; 9294; -.
DR RGD; 68334; S1pr2.
DR eggNOG; ENOG502QVQY; Eukaryota.
DR GeneTree; ENSGT01050000244887; -.
DR HOGENOM; CLU_047979_1_0_1; -.
DR InParanoid; P47752; -.
DR OMA; CPVRACP; -.
DR OrthoDB; 981486at2759; -.
DR PhylomeDB; P47752; -.
DR TreeFam; TF330052; -.
DR Reactome; R-RNO-418594; G alpha (i) signalling events.
DR Reactome; R-RNO-419408; Lysosphingolipid and LPA receptors.
DR PRO; PR:P47752; -.
DR Proteomes; UP000002494; Chromosome 8.
DR Bgee; ENSRNOG00000020653; Expressed in lung and 19 other tissues.
DR Genevisible; P47752; RN.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR GO; GO:0098794; C:postsynapse; IEA:GOC.
DR GO; GO:0008528; F:G protein-coupled peptide receptor activity; IDA:MGI.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IDA:RGD.
DR GO; GO:0001664; F:G protein-coupled receptor binding; ISO:RGD.
DR GO; GO:0005178; F:integrin binding; ISO:RGD.
DR GO; GO:0038036; F:sphingosine-1-phosphate receptor activity; ISO:RGD.
DR GO; GO:0031532; P:actin cytoskeleton reorganization; ISO:RGD.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0060079; P:excitatory postsynaptic potential; IEA:Ensembl.
DR GO; GO:0046847; P:filopodium assembly; ISO:RGD.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:MGI.
DR GO; GO:0090394; P:negative regulation of excitatory postsynaptic potential; ISO:RGD.
DR GO; GO:0014912; P:negative regulation of smooth muscle cell migration; IGI:MGI.
DR GO; GO:1904706; P:negative regulation of vascular associated smooth muscle cell proliferation; IGI:MGI.
DR GO; GO:1903142; P:positive regulation of establishment of endothelial barrier; ISO:RGD.
DR GO; GO:0010800; P:positive regulation of peptidyl-threonine phosphorylation; ISO:RGD.
DR GO; GO:0019222; P:regulation of metabolic process; IBA:GO_Central.
DR GO; GO:0003376; P:sphingosine-1-phosphate receptor signaling pathway; ISO:RGD.
DR InterPro; IPR004063; EDG5_rcpt.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR004061; S1P_rcpt.
DR PANTHER; PTHR22750:SF17; PTHR22750:SF17; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR01525; EDG5RECEPTOR.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01523; S1PRECEPTOR.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; G-protein coupled receptor; Glycoprotein; Lipoprotein;
KW Membrane; Palmitate; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..352
FT /note="Sphingosine 1-phosphate receptor 2"
FT /id="PRO_0000069429"
FT TOPO_DOM 1..34
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 35..59
FT /note="Helical; Name=1"
FT /evidence="ECO:0000250"
FT TOPO_DOM 60..66
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 67..95
FT /note="Helical; Name=2"
FT /evidence="ECO:0000250"
FT TOPO_DOM 96..109
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 110..128
FT /note="Helical; Name=3"
FT /evidence="ECO:0000250"
FT TOPO_DOM 129..147
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 148..173
FT /note="Helical; Name=4"
FT /evidence="ECO:0000250"
FT TOPO_DOM 174..189
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 190..210
FT /note="Helical; Name=5"
FT /evidence="ECO:0000250"
FT TOPO_DOM 211..233
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 234..255
FT /note="Helical; Name=6"
FT /evidence="ECO:0000250"
FT TOPO_DOM 256..271
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 272..292
FT /note="Helical; Name=7"
FT /evidence="ECO:0000250"
FT TOPO_DOM 293..352
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT LIPID 305
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250"
FT CARBOHYD 19
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 352 AA; 38735 MW; 9C933A18E756CE1E CRC64;
MGGLYSEYLN PEKVQEHYNY TKETLDMQET PSRKVASAFI IILCCAIVVE NLLVLIAVAR
NSKFHSAMYL FLGNLAASDL LAGVAFVANT LLSGPVTLSL TPLQWFAREG SAFITLSASV
FSLLAIAIER QVAIAKVKLY GSDKSCRMLM LIGASWLISL ILGGLPILGW NCLDHLEACS
TVLPLYAKHY VLCVVTIFSV ILLAIVALYV RIYFVVRSSH ADVAGPQTLA LLKTVTIVLG
VFIICWLPAF SILLLDSTCP VRACPVLYKA HYFFAFATLN SLLNPVIYTW RSRDLRREVL
RPLLCWRQGK GATGRRGGNP GHRLLPLRSS SSLERGLHMP TSPTFLEGNT VV
