ID   BEM2_ASHGO              Reviewed;        2071 AA.
AC   Q9HF75;
DT   31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=GTPase-activating protein BEM2;
GN   Name=BEM2; OrderedLocusNames=AGR144C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=10751152; DOI=10.1242/jcs.113.9.1611;
RA   Wendland J., Philippsen P.;
RT   "Determination of cell polarity in germinated spores and hyphal tips of the
RT   filamentous ascomycete Ashbya gossypii requires a rhoGAP homolog.";
RL   J. Cell Sci. 113:1611-1621(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: GTPase-activating protein (GAP) for RHO proteins. Required
CC       for polarized growth and maintenance of cell polarity.
CC       {ECO:0000269|PubMed:10751152}.
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DR   EMBL; AF195007; AAG43463.1; -; Genomic_DNA.
DR   EMBL; AE016820; AAS54634.1; -; Genomic_DNA.
DR   RefSeq; NP_986810.1; NM_211872.1.
DR   AlphaFoldDB; Q9HF75; -.
DR   SMR; Q9HF75; -.
DR   STRING; 33169.AAS54634; -.
DR   EnsemblFungi; AAS54634; AAS54634; AGOS_AGR144C.
DR   GeneID; 4623112; -.
DR   KEGG; ago:AGOS_AGR144C; -.
DR   eggNOG; KOG1450; Eukaryota.
DR   HOGENOM; CLU_002085_0_0_1; -.
DR   InParanoid; Q9HF75; -.
DR   OMA; LEDDRWF; -.
DR   Proteomes; UP000000591; Chromosome VII.
DR   GO; GO:0005938; C:cell cortex; IEA:EnsemblFungi.
DR   GO; GO:0005934; C:cellular bud tip; IEA:EnsemblFungi.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0000131; C:incipient cellular bud site; IEA:EnsemblFungi.
DR   GO; GO:0043332; C:mating projection tip; IEA:EnsemblFungi.
DR   GO; GO:0005886; C:plasma membrane; IEA:EnsemblFungi.
DR   GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro.
DR   GO; GO:0030036; P:actin cytoskeleton organization; IEA:EnsemblFungi.
DR   GO; GO:0044879; P:mitotic morphogenesis checkpoint signaling; IEA:EnsemblFungi.
DR   GO; GO:0035024; P:negative regulation of Rho protein signal transduction; IEA:EnsemblFungi.
DR   GO; GO:0043087; P:regulation of GTPase activity; IBA:GO_Central.
DR   GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR   CDD; cd06224; REM; 1.
DR   Gene3D; 1.10.555.10; -; 1.
DR   Gene3D; 1.10.840.10; -; 1.
DR   Gene3D; 2.30.29.30; -; 1.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR001849; PH_domain.
DR   InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR   InterPro; IPR023578; Ras_GEF_dom_sf.
DR   InterPro; IPR001895; RASGEF_cat_dom.
DR   InterPro; IPR036964; RASGEF_cat_dom_sf.
DR   InterPro; IPR008936; Rho_GTPase_activation_prot.
DR   InterPro; IPR000198; RhoGAP_dom.
DR   Pfam; PF00617; RasGEF; 1.
DR   Pfam; PF00618; RasGEF_N; 1.
DR   Pfam; PF00620; RhoGAP; 1.
DR   SMART; SM00233; PH; 1.
DR   SMART; SM00147; RasGEF; 1.
DR   SMART; SM00229; RasGEFN; 1.
DR   SMART; SM00324; RhoGAP; 1.
DR   SUPFAM; SSF48350; SSF48350; 1.
DR   SUPFAM; SSF48366; SSF48366; 2.
DR   PROSITE; PS50003; PH_DOMAIN; 1.
DR   PROSITE; PS50009; RASGEF_CAT; 1.
DR   PROSITE; PS50238; RHOGAP; 1.
PE   4: Predicted;
KW   GTPase activation; Reference proteome.
FT   CHAIN           1..2071
FT                   /note="GTPase-activating protein BEM2"
FT                   /id="PRO_0000068857"
FT   DOMAIN          471..738
FT                   /note="Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT   DOMAIN          1751..1853
FT                   /note="PH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT   DOMAIN          1872..2070
FT                   /note="Rho-GAP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT   REGION          1..115
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          171..195
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          787..819
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1645..1676
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1702..1738
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        14..114
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        181..195
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1651..1676
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1702..1734
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   2071 AA;  232152 MW;  1CBA152E9AB8A148 CRC64;
     MPLKWAARNK KPPSAPQSCA SKPSSASQSS CVDERISATP RSSISSNSSP NSKNNMSRHS
     HSNGSVYSDE TTLKTAQTHY TQQGQQAKPQ QHTQQQQQQP QTPMQLQVPT GQAHKRTLTC
     EDMKAGARCE EQVSPCSQPA GSPVRRGGGL NGETYDGTVF RLGWVNKAQG AAPAREGRYS
     HQPTASLSSI GSERPHFTGG GTSGYQYVAT AYRLHRAQLK GCILNLYKSG LTNVKYFDPA
     LEPSAAALQM HQERQEMPLL QPPLPSEAVP APSILEASME SGELRLEYLS EAYPHPDLQL
     DKKDGKILSG SLESLCHAVL FMPTTDAKRV TDILLLLPLL DDFTRVLNYF NLFGKVFSKH
     HPAGAAGADD LNQNYNISNE TDRQLTLRLA TVVQTVLDMF PGFLLDDKIF QSLVILLDTI
     SFHDEDTSQE LKVAIAEKQT VLVKLTGFAN EPIQSAKLDV LIKVQSFLKL DTEKVANQIH
     KINLTFNRVW SPQADYSLLY DSQYTQKHVE LNPLVFFNDK NVQYLSRLMV SHIFCEETGF
     TPKKRAEVLT KWVQLGCKFE RLGDMVSWLA IATVICSIPV LRLTRTWQYV PDSYLKIIFK
     DWVPTIVQLD RRQMSSKSMN SVFILAPPNL NDAFVRDNVI PYFGDLVIHS DDLPRDSKYK
     YLEKKIRRTK NAFYKWQQRL DQAFAQDRDS ASSFTDSLHL DEEEHDVADF YQYWRFHMNL
     PPMNIETIME MSLKMEPPSI NQQTYSKTYS TRSALISGAY LPTLFTTLLP SYSLFPQELL
     IAAASTPSTK NNNSSQASNR ISQLSVNSTP HSNASSSSAA SAVTGIDNID VPITKEISSK
     LSNKQVLLKF IRDMFNVDIN VFHISDDVIF KSIRDYEAKS RPTSVVIESP KRLSLLSSVS
     PDVSAVSSAL ENLDLFKNFN SSSDDIAEFT VQVVLKCASL EKIFDILVLT SRVFSNLVTT
     TDLVSYFNSE KARREKSGAQ HNGQHSIGLL DFALISLIMD NELFAETFFN NYKSFTTTLC
     VLENLAKRFI GAKSSAISIS LINKLRNSES SRQIPPSTTS NQFSASGIFK PSYDELKFPV
     WDLKVTSVEG CPLDYLAKIQ IGVLESLYHL IREHYADFTD DLANNKTFLD ILKIINQEVY
     DEWDKRLDDL RNNNNSSQKR KNSCDDNSSA KITFHVNDAR PENSNENKRG AATNLGDSSL
     AALEKLQCTL QDLYVKIKSS YQRQLYRPLG VTRNCRKVHD MLCQFQPQTS MSALIMNGSS
     DTLDKMVTEF QALKHTDYDD IINWIYKLDH FITSKLKLVS NQDWIQVSQI LESLSNDSLV
     ALFNYPLHAE SNNVIASGSS QLDDLQILDI FTWLSTLESG SAHIIDKFPA SVQLIVRLHL
     SLTKFFTVHI AHLHSTYEAR VNTCSLILEI LNFVHVKNAN VNLFHSDDAG EGSMATISPH
     VPSFIETAIE NAIISPESRF FEVSWKQAYK TISEKDEKLT FIGSVLTGLD KSTAHFLDAD
     NRQPVRPKNF SPCPGWFISR LLEITGLVPN MSIENSKMIN FDKRRFINNI VINYQDLIPN
     TEQLPSHDDE KSAHQFGSIL FHYGTESSIK AFRKASKEAA SNEARKLKFQ AMGLFNDILV
     TEVYKVQRDQ KKQEQLTVQE HEAKRSVLIQ HPNKVSVSSA SSSVSGSSSG STARTSNPAH
     AAYALNMAGS LSISAARHGR SSVSSRSSVI SNTATATSPA SGASPNQTST SHHGGMGKKI
     GGFLRRPFSI SGFTSSSSQY TTTSVVLSGV QANGSISPYE LPELTSEIQD TKIVTVIKTF
     EIKSCIQINN YRQDPDMMHC FKIVMEDGTQ HTLQCMDDAD MHEWMKAITL SKRYSFHSKR
     FKGKTSNKIF GVPVEDVCER EGALIPNIIV KLLDEIELRG LDEVGLYRVP GSVGSINALK
     NAFDDEGAVH NTFTLEDDRW FEINTIAGCF KLYLRELPES LFTNEKVDEF VNIMTAYKNH
     EVDLSQFQNG IKTLLSTLPV FNYHILKRLF LHLNRVHQHV ENNRMDASNL AIVFSMSFIN
     QDDLASTMGP TLGLLQMLLQ HLIRNPEHYF T