S20A2_RAT
ID S20A2_RAT Reviewed; 656 AA.
AC Q63488;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Sodium-dependent phosphate transporter 2;
DE AltName: Full=Phosphate transporter 2;
DE Short=PiT-2;
DE AltName: Full=Receptor for amphotropic viruses 1;
DE Short=RAM-1;
DE AltName: Full=Solute carrier family 20 member 2;
GN Name=Slc20a2; Synonyms=Pit2, Ram1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8278411; DOI=10.1073/pnas.91.1.78;
RA Miller D.G., Edwards R.H., Miller A.D.;
RT "Cloning of the cellular receptor for amphotropic murine retroviruses
RT reveals homology to that for gibbon ape leukemia virus.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:78-82(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Heart;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION AS SODIUM-PHOSPHATE SYMPORTER, AND TISSUE SPECIFICITY.
RX PubMed=8041748; DOI=10.1073/pnas.91.15.7071;
RA Kavanaugh M.P., Miller D.G., Zhang W., Law W., Kozak S.L., Kabat D.,
RA Miller A.D.;
RT "Cell-surface receptors for gibbon ape leukemia virus and amphotropic
RT murine retrovirus are inducible sodium-dependent phosphate symporters.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:7071-7075(1994).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-253; SER-256 AND SER-259, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Sodium-phosphate symporter which seems to play a fundamental
CC housekeeping role in phosphate transport by absorbing phosphate from
CC interstitial fluid for normal cellular functions such as cellular
CC metabolism, signal transduction, and nucleic acid and lipid synthesis.
CC In vitro, sodium-dependent phosphate uptake is not significantly
CC affected by acidic and alkaline conditions, however sodium-independent
CC phosphate uptake occurs at acidic conditions. May play a role in
CC extracellular matrix and cartilage calcification as well as in vascular
CC calcification. Functions as a retroviral receptor (By similarity).
CC {ECO:0000250, ECO:0000269|PubMed:8041748}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Widely expressed with highest levels in liver,
CC heart and brain. {ECO:0000269|PubMed:8041748}.
CC -!- SIMILARITY: Belongs to the inorganic phosphate transporter (PiT) (TC
CC 2.A.20) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L19931; AAA16532.1; -; mRNA.
DR EMBL; BC070908; AAH70908.1; -; mRNA.
DR PIR; A49579; A49579.
DR RefSeq; NP_058919.1; NM_017223.2.
DR RefSeq; XP_008769573.1; XM_008771351.2.
DR RefSeq; XP_008769574.1; XM_008771352.2.
DR RefSeq; XP_017455543.1; XM_017600054.1.
DR AlphaFoldDB; Q63488; -.
DR SMR; Q63488; -.
DR BioGRID; 248142; 1.
DR STRING; 10116.ENSRNOP00000026418; -.
DR GlyGen; Q63488; 1 site.
DR iPTMnet; Q63488; -.
DR PhosphoSitePlus; Q63488; -.
DR PaxDb; Q63488; -.
DR PRIDE; Q63488; -.
DR Ensembl; ENSRNOT00000026418; ENSRNOP00000026418; ENSRNOG00000019490.
DR GeneID; 29502; -.
DR KEGG; rno:29502; -.
DR UCSC; RGD:3699; rat.
DR CTD; 6575; -.
DR RGD; 3699; Slc20a2.
DR eggNOG; KOG2493; Eukaryota.
DR GeneTree; ENSGT00390000014879; -.
DR HOGENOM; CLU_015355_3_1_1; -.
DR InParanoid; Q63488; -.
DR OMA; PIGWAMR; -.
DR OrthoDB; 712010at2759; -.
DR PhylomeDB; Q63488; -.
DR Reactome; R-RNO-427652; Sodium-coupled phosphate cotransporters.
DR PRO; PR:Q63488; -.
DR Proteomes; UP000002494; Chromosome 16.
DR Bgee; ENSRNOG00000019490; Expressed in heart and 20 other tissues.
DR ExpressionAtlas; Q63488; baseline and differential.
DR Genevisible; Q63488; RN.
DR GO; GO:0016021; C:integral component of membrane; TAS:RGD.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0035435; P:phosphate ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR001204; Phos_transporter.
DR PANTHER; PTHR11101; PTHR11101; 1.
DR Pfam; PF01384; PHO4; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Host-virus interaction; Ion transport;
KW Membrane; Phosphate transport; Phosphoprotein; Receptor;
KW Reference proteome; Sodium; Sodium transport; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..656
FT /note="Sodium-dependent phosphate transporter 2"
FT /id="PRO_0000341270"
FT TOPO_DOM 1..5
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 27..46
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 68..86
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..109
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 131..142
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 164..190
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 212..213
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 235..483
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 484..504
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 505..531
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 532..552
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 553..572
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 573..586
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 587..594
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 595..610
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 611..622
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 623..643
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 644..655
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT MOD_RES 253
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 256
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 259
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 268
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q08357"
FT MOD_RES 316
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q08357"
FT MOD_RES 385
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q08357"
FT CARBOHYD 81
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 656 AA; 70748 MW; 448AAB3EC5D64DC1 CRC64;
MAMDGYLWMV ILGFIIAFIL AFSVGANDVA NSFGTAVGSG VVTLRQACIL ASIFETTGSV
LLGAKVGETI RKGIIDVNLY NETVETLMAG EVSAMVGSAV WQLIASFLRL PISGTHCIVG
STIGFSLVAI GPKGVQWMEL VKIVASWFIS PLLSGFMSGV LFILIRMFIL TKEDPVPNGL
QALPLFYAAT IAINVFSIMY TGAPVLGLSL PIWAIALISF GVALLFAFFV WLFVCPWMKR
KIAGRLEKES ALSRASDESL RKVQEAESPV FKELPGAKAS DDSAVPLTSL AGEAAGASEG
TSAGNHPRAS YGRALSMTHG SAKSPISNGT FGFEGHMRND GHVYHTVHKD SGLYKDLLHK
IHVDKGPEEK PAQENNYRLL RRNNSYTCYT AAICGMPVHA TFRASDTSSA PEDSEKLVGD
TVSYSKKRLR YDSYSSYCNA VAEAEIEAEE GGVEMRLASE LTDPDQPHED PAEDEKEEKD
SAEVHLLFHF LQVLTACFGS FAHGGNDVSN AIGPLVALWL IYKQGGVTQE AATPVWLLFY
GGVGICTGLW VWGRRVIQTM GKDLTPITPS SGFTIELASA FTVVIASNIG LPVSTTHCKV
GSVVAVGWIR SRKAVDWRLF RNIFIAWFVT VPVAGLFSAA IMALLMYICG FVSSSR
