BEM3_ASHGO
ID BEM3_ASHGO Reviewed; 1013 AA.
AC Q74ZH7; Q9HF64;
DT 31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2013, sequence version 2.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=GTPase-activating protein BEM3;
GN Name=BEM3; OrderedLocusNames=AGR230W;
OS Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS (Yeast) (Eremothecium gossypii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX NCBI_TaxID=284811;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Wendland J., Philippsen P.;
RT "Isolation and characterization of the Ashbya gossypii BEM3 homolog.";
RL Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=15001715; DOI=10.1126/science.1095781;
RA Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA Gaffney T.D., Philippsen P.;
RT "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT cerevisiae genome.";
RL Science 304:304-307(2004).
RN [3]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 61.
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=23749448; DOI=10.1534/g3.112.002881;
RA Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT loci, numerous translocations, lack of transposons, and distinct gene
RT duplications.";
RL G3 (Bethesda) 3:1225-1239(2013).
CC -!- FUNCTION: GTPase-activating protein (GAP) for CDC42 and less
CC efficiently for RHO1. Negative regulator of the pheromone-response
CC pathway through the STE20 protein kinase (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
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DR EMBL; AF210624; AAG41239.1; -; Genomic_DNA.
DR EMBL; AE016820; AAS54720.2; -; Genomic_DNA.
DR RefSeq; NP_986896.2; NM_211958.2.
DR AlphaFoldDB; Q74ZH7; -.
DR SMR; Q74ZH7; -.
DR STRING; 33169.AAS54720; -.
DR EnsemblFungi; AAS54720; AAS54720; AGOS_AGR230W.
DR GeneID; 4623198; -.
DR KEGG; ago:AGOS_AGR230W; -.
DR eggNOG; KOG4269; Eukaryota.
DR HOGENOM; CLU_010436_0_0_1; -.
DR InParanoid; Q74ZH7; -.
DR OMA; NNHIHSP; -.
DR Proteomes; UP000000591; Chromosome VII.
DR GO; GO:0005938; C:cell cortex; IEA:EnsemblFungi.
DR GO; GO:0005934; C:cellular bud tip; IEA:EnsemblFungi.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0000131; C:incipient cellular bud site; IEA:EnsemblFungi.
DR GO; GO:0043332; C:mating projection tip; IEA:EnsemblFungi.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; IEA:EnsemblFungi.
DR GO; GO:0030010; P:establishment of cell polarity; IEA:EnsemblFungi.
DR GO; GO:0035024; P:negative regulation of Rho protein signal transduction; IEA:EnsemblFungi.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IEA:EnsemblFungi.
DR GO; GO:0043087; P:regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0031106; P:septin ring organization; IEA:EnsemblFungi.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.555.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR Gene3D; 3.30.1520.10; -; 1.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR001683; PX_dom.
DR InterPro; IPR036871; PX_dom_sf.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00787; PX; 1.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00233; PH; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR SUPFAM; SSF64268; SSF64268; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 3: Inferred from homology;
KW Cytoplasm; GTPase activation; Reference proteome.
FT CHAIN 1..1013
FT /note="GTPase-activating protein BEM3"
FT /id="PRO_0000056727"
FT DOMAIN 555..662
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 799..1013
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REGION 90..197
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 258..277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 282..301
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 307..421
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 702..726
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 139..164
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 165..179
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 180..197
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 282..300
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 329..421
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1013 AA; 111660 MW; 4D24D96D01262083 CRC64;
MGDGSDAERS GGTSSSSALE LLAQYEQHIM ERGRTLEAIE GHGGERLGPT YEELVEENVQ
LRRELQGQRE EIEHLRKTIS LLASGRSGAT VVEQQVRPEP SPSVRELALP PRSADRRKNT
KNLSLAPVGH EVPSTDRLRV SPQEATSGAQ QVPLLTSSKS AEILVSKSPD EDRHLMSPRK
TISRSSSSYS NTLGSPATSV LYKNSRISIT SPCKSNSTSK AASVLSLPEN NTSTENAPHS
PHRIDNELDL LTVEPQDGSR YDTERAGGPG PLSPESIVYS DSDLQEHQPS DLSSTTRTDL
GKFRDMVDTT FNAEDNPTGS RDKETGTEME IATLQNTPSR QHESSLVTSP QASRSSITTP
VVDPTNTSEP SSLSAAKFGS MSTATSSNKR SKGMGTPSVE HSAKSYSQHS GSPHSNSHQS
KKADIPLFVQ PEELGTIRIE VISTLYHEPG NAASILFSVV DKKSSKEMFK FAKTFTRIAE
FDTFIRNNME SLAVPPLPDK HMFASNVPVK VDSRREKLND YFASLLYLSP LPFNPALKLA
QFISTDPVMN PITGEFAKEG MLLVRKSKTL GSTTTWRIRY CTVEGSIMHL HDHMIDTDTI
KLTHSTIELQ ANLPDDKYGT KNGFILNEHK KSGLSSSTKY YFCAETPKER EQWISVLTTL
CDGPGGTAAI PSINSKSEAS SLFEQTSISD SSYLGPIANL EAMDATSPTR PNDPNPVSLT
SEEEKEVKRR RMKSFFPFKK LATTPTPYAA GNDNASIFSQ DDDSPVNATN ESGISRSLQS
MNLQAQYNAV FGADLRSCLQ LSSHPYQGKY EIPSVVFRTL EFLYKNRGIQ EEGIFRLSGS
SSLIKSLQEQ FDKEYDVDLC NYNDKVSVTP GNENQGGLYV DVNTVSGLLK LYLRKLPHMI
FGDAAYMDFK RIVERNGDDS KLIALEFRAL VNSGRIAKEY VALMYALFEL LVKITENSKY
NKMNLRNLCI VFSPTLNIPV NILHPFITDF GCIFQDKAPM ENGPPVNIHI PQI
