S22A3_MOUSE
ID S22A3_MOUSE Reviewed; 551 AA.
AC Q9WTW5; Q9R209;
DT 19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Solute carrier family 22 member 3;
DE AltName: Full=Organic cation transporter 3;
GN Name=Slc22a3; Synonyms=Oct3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=CD-1; TISSUE=Placenta;
RX PubMed=9933568; DOI=10.1006/geno.1998.5639;
RA Verhaagh S., Schweifer N., Barlow D.P., Zwart R.;
RT "Cloning of the mouse and human solute carrier 22a3 (Slc22a3/SLC22A3)
RT identifies a conserved cluster of three organic cation transporters on
RT mouse chromosome 17 and human 6q26-q27.";
RL Genomics 55:209-218(1999).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE, FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Kidney;
RX PubMed=10966924; DOI=10.1152/ajprenal.2000.279.3.f449;
RA Wu X., Huang W., Ganapathy M.E., Wang H., Kekuda R., Conway S.J.,
RA Leibach F.H., Ganapathy V.;
RT "Structure, function, and regional distribution of the organic cation
RT transporter OCT3 in the kidney.";
RL Am. J. Physiol. 279:F449-F458(2000).
CC -!- FUNCTION: Mediates potential-dependent transport of a variety of
CC organic cations. May play a significant role in the disposition of
CC cationic neurotoxins and neurotransmitters in the brain.
CC {ECO:0000269|PubMed:10966924, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Highly expressed in placenta and in kidney cortex,
CC but not in medulla. Low levels found in brain. In the kidney, expressed
CC specifically in the proximal and distal convoluted tubules and within
CC Bowman capsule, but not in the glomerulus.
CC {ECO:0000269|PubMed:10966924, ECO:0000269|PubMed:9933568, ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Levels are high during gestation, but decrease
CC greatly towards the end of gestation. {ECO:0000269|PubMed:9933568,
CC ECO:0000305}.
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
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DR EMBL; AF078750; AAD20978.1; -; mRNA.
DR EMBL; AF078748; AAD20238.1; -; Genomic_DNA.
DR EMBL; AF082566; AAD53007.1; -; mRNA.
DR CCDS; CCDS28391.1; -.
DR RefSeq; NP_035525.1; NM_011395.2.
DR AlphaFoldDB; Q9WTW5; -.
DR SMR; Q9WTW5; -.
DR STRING; 10090.ENSMUSP00000024595; -.
DR ChEMBL; CHEMBL2073684; -.
DR GlyGen; Q9WTW5; 5 sites.
DR iPTMnet; Q9WTW5; -.
DR PhosphoSitePlus; Q9WTW5; -.
DR SwissPalm; Q9WTW5; -.
DR MaxQB; Q9WTW5; -.
DR PaxDb; Q9WTW5; -.
DR PRIDE; Q9WTW5; -.
DR ProteomicsDB; 260884; -.
DR Antibodypedia; 20034; 213 antibodies from 34 providers.
DR DNASU; 20519; -.
DR Ensembl; ENSMUST00000024595; ENSMUSP00000024595; ENSMUSG00000023828.
DR GeneID; 20519; -.
DR KEGG; mmu:20519; -.
DR UCSC; uc008aku.1; mouse.
DR CTD; 6581; -.
DR MGI; MGI:1333817; Slc22a3.
DR VEuPathDB; HostDB:ENSMUSG00000023828; -.
DR eggNOG; KOG0255; Eukaryota.
DR GeneTree; ENSGT00940000160810; -.
DR HOGENOM; CLU_001265_33_5_1; -.
DR InParanoid; Q9WTW5; -.
DR OMA; QMRRNTL; -.
DR OrthoDB; 762280at2759; -.
DR PhylomeDB; Q9WTW5; -.
DR TreeFam; TF315847; -.
DR Reactome; R-MMU-2161517; Abacavir transmembrane transport.
DR Reactome; R-MMU-549127; Organic cation transport.
DR BioGRID-ORCS; 20519; 3 hits in 71 CRISPR screens.
DR ChiTaRS; Slc22a3; mouse.
DR PRO; PR:Q9WTW5; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q9WTW5; protein.
DR Bgee; ENSMUSG00000023828; Expressed in placenta labyrinth and 94 other tissues.
DR ExpressionAtlas; Q9WTW5; baseline and differential.
DR Genevisible; Q9WTW5; MM.
DR GO; GO:0005887; C:integral component of plasma membrane; TAS:MGI.
DR GO; GO:0043025; C:neuronal cell body; IDA:ARUK-UCL.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0098793; C:presynapse; IEA:GOC.
DR GO; GO:0005330; F:dopamine:sodium symporter activity; ISO:MGI.
DR GO; GO:0008504; F:monoamine transmembrane transporter activity; ISO:MGI.
DR GO; GO:0005326; F:neurotransmitter transmembrane transporter activity; IMP:ARUK-UCL.
DR GO; GO:0008514; F:organic anion transmembrane transporter activity; ISO:MGI.
DR GO; GO:0015101; F:organic cation transmembrane transporter activity; IDA:MGI.
DR GO; GO:0015651; F:quaternary ammonium group transmembrane transporter activity; IDA:MGI.
DR GO; GO:0019534; F:toxin transmembrane transporter activity; ISO:MGI.
DR GO; GO:0022857; F:transmembrane transporter activity; IDA:MGI.
DR GO; GO:0015872; P:dopamine transport; IMP:ARUK-UCL.
DR GO; GO:0090494; P:dopamine uptake; ISO:MGI.
DR GO; GO:0051625; P:epinephrine uptake; ISO:MGI.
DR GO; GO:0051649; P:establishment of localization in cell; IMP:MGI.
DR GO; GO:0051608; P:histamine transport; IMP:ARUK-UCL.
DR GO; GO:0051615; P:histamine uptake; IMP:MGI.
DR GO; GO:0015844; P:monoamine transport; IMP:MGI.
DR GO; GO:0015718; P:monocarboxylic acid transport; IMP:ARUK-UCL.
DR GO; GO:0006836; P:neurotransmitter transport; IMP:ARUK-UCL.
DR GO; GO:0051620; P:norepinephrine uptake; ISO:MGI.
DR GO; GO:0015711; P:organic anion transport; ISO:MGI.
DR GO; GO:0015695; P:organic cation transport; IDA:MGI.
DR GO; GO:0015850; P:organic hydroxy compound transport; IMP:ARUK-UCL.
DR GO; GO:0015697; P:quaternary ammonium group transport; IDA:MGI.
DR GO; GO:0032098; P:regulation of appetite; IMP:MGI.
DR GO; GO:0006837; P:serotonin transport; IMP:ARUK-UCL.
DR GO; GO:0051610; P:serotonin uptake; ISO:MGI.
DR GO; GO:1901998; P:toxin transport; ISO:MGI.
DR GO; GO:0042908; P:xenobiotic transport; ISO:MGI.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004749; Orgcat_transp/SVOP.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00898; 2A0119; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..551
FT /note="Solute carrier family 22 member 3"
FT /id="PRO_0000220504"
FT TRANSMEM 21..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 464..484
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 493..513
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 72
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 99
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 114
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 199
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 317
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 551 AA; 61053 MW; C4D66AAC2EFCFBDB CRC64;
MPTFDQALRK AGEFGRFQRR VFLLLCLTGV TFAFLFVGVV FLGSQPDYYW CRGPRATALA
ERCAWSPEEE WNLTTPELHV PAERRGQGHC HRYLLEATNT SSELSCDPLT AFPNRSAPLV
SCSGDWRYVE THSTIVSQFD LVCSNAWMLD LTQAILNLGF LAGAFTLGYA ADRYGRLIIY
LISCFGVGIT GVVVAFAPNF SVFVIFRFLQ GVFGKGAWMT CFVIVTEIVG SKQRRIVGIV
IQMFFTLGII ILPGIAYFTP SWQGIQLAIS LPSFLFLLYY WVVPESPRWL ITRKQGEKAL
QILRRVAKCN GKHLSSNYSE ITVTDEEVSN PSCLDLVRTP QMRKCTLILM FAWFTSAVVY
QGLVMRLGLI GGNLYIDFFI SGLVELPGAL LILLTIERLG RRLPFAASNI VAGVSCLVTA
FLPEGIPWLR TTVATLGRLG ITMAFEIVYL VNSELYPTTL RNFGVSLCSG LCDFGGIIAP
FLLFRLAAIW LELPLIIFGI LASVCGGLVM LLPETKGIAL PETVEDVEKL GSSQLHQCGR
KKKTQVSTSD V
