S22A3_RAT
ID S22A3_RAT Reviewed; 551 AA.
AC O88446;
DT 19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Solute carrier family 22 member 3;
DE AltName: Full=Organic cation transporter 3;
GN Name=Slc22a3; Synonyms=Oct3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Placenta;
RX PubMed=9632645; DOI=10.1074/jbc.273.26.15971;
RA Kekuda R., Prasad P.D., Wu X., Wang H., Fei Y.-J., Leibach F.H.,
RA Ganapathy V.;
RT "Cloning and functional characterization of a potential-sensitive,
RT polyspecific organic cation transporter (OCT3) most abundantly expressed in
RT placenta.";
RL J. Biol. Chem. 273:15971-15979(1998).
RN [2] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Placenta;
RX PubMed=9830022; DOI=10.1074/jbc.273.49.32776;
RA Wu X., Kekuda R., Huang W., Fei Y.-J., Leibach F.H., Chen J., Conway S.J.,
RA Ganapathy V.;
RT "Identity of the organic cation transporter OCT3 as the extraneuronal
RT monoamine transporter (uptake2) and evidence for the expression of the
RT transporter in the brain.";
RL J. Biol. Chem. 273:32776-32786(1998).
CC -!- FUNCTION: Mediates potential-dependent transport of a variety of
CC organic cations. May play a significant role in the disposition of
CC cationic neurotoxins and neurotransmitters in the brain.
CC {ECO:0000269|PubMed:9632645, ECO:0000269|PubMed:9830022, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Highly expressed in placenta. Moderate expression
CC in intestine, heart and brain. In the brain, widely expressed,
CC especially in hippocampus, cerebellum and cerebral cortex. Expression
CC is low in kidney and lung and undetectable in liver.
CC {ECO:0000269|PubMed:9632645, ECO:0000269|PubMed:9830022, ECO:0000305}.
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF055286; AAC40150.1; -; mRNA.
DR RefSeq; NP_062103.1; NM_019230.1.
DR AlphaFoldDB; O88446; -.
DR SMR; O88446; -.
DR STRING; 10116.ENSRNOP00000029748; -.
DR BindingDB; O88446; -.
DR ChEMBL; CHEMBL1770033; -.
DR TCDB; 2.A.1.19.6; the major facilitator superfamily (mfs).
DR GlyGen; O88446; 5 sites.
DR PaxDb; O88446; -.
DR Ensembl; ENSRNOT00000037369; ENSRNOP00000029748; ENSRNOG00000022946.
DR GeneID; 29504; -.
DR KEGG; rno:29504; -.
DR CTD; 6581; -.
DR RGD; 3701; Slc22a3.
DR eggNOG; KOG0255; Eukaryota.
DR GeneTree; ENSGT00940000160810; -.
DR HOGENOM; CLU_001265_33_5_1; -.
DR InParanoid; O88446; -.
DR OMA; QMRRNTL; -.
DR OrthoDB; 762280at2759; -.
DR PhylomeDB; O88446; -.
DR TreeFam; TF315847; -.
DR Reactome; R-RNO-2161517; Abacavir transmembrane transport.
DR Reactome; R-RNO-549127; Organic cation transport.
DR PRO; PR:O88446; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Bgee; ENSRNOG00000022946; Expressed in thymus and 15 other tissues.
DR Genevisible; O88446; RN.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043025; C:neuronal cell body; ISO:RGD.
DR GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR GO; GO:0098793; C:presynapse; IEA:GOC.
DR GO; GO:0005330; F:dopamine:sodium symporter activity; IDA:RGD.
DR GO; GO:0008504; F:monoamine transmembrane transporter activity; ISO:RGD.
DR GO; GO:0005326; F:neurotransmitter transmembrane transporter activity; ISO:RGD.
DR GO; GO:0008514; F:organic anion transmembrane transporter activity; IDA:ARUK-UCL.
DR GO; GO:0015101; F:organic cation transmembrane transporter activity; IDA:RGD.
DR GO; GO:0015651; F:quaternary ammonium group transmembrane transporter activity; IMP:RGD.
DR GO; GO:0019534; F:toxin transmembrane transporter activity; IDA:RGD.
DR GO; GO:0022857; F:transmembrane transporter activity; ISO:RGD.
DR GO; GO:0015872; P:dopamine transport; IDA:RGD.
DR GO; GO:0090494; P:dopamine uptake; ISO:RGD.
DR GO; GO:0051625; P:epinephrine uptake; ISO:RGD.
DR GO; GO:0051608; P:histamine transport; ISO:RGD.
DR GO; GO:0051615; P:histamine uptake; ISO:RGD.
DR GO; GO:0015844; P:monoamine transport; ISO:RGD.
DR GO; GO:0015718; P:monocarboxylic acid transport; ISO:RGD.
DR GO; GO:0006836; P:neurotransmitter transport; ISO:RGD.
DR GO; GO:0051620; P:norepinephrine uptake; ISO:RGD.
DR GO; GO:0015711; P:organic anion transport; IDA:ARUK-UCL.
DR GO; GO:0015695; P:organic cation transport; IDA:RGD.
DR GO; GO:0015850; P:organic hydroxy compound transport; ISO:RGD.
DR GO; GO:0015697; P:quaternary ammonium group transport; IMP:RGD.
DR GO; GO:0032098; P:regulation of appetite; ISO:RGD.
DR GO; GO:0006837; P:serotonin transport; ISO:RGD.
DR GO; GO:0051610; P:serotonin uptake; ISO:RGD.
DR GO; GO:1901998; P:toxin transport; ISO:RGD.
DR GO; GO:0042908; P:xenobiotic transport; ISO:RGD.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004749; Orgcat_transp/SVOP.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00898; 2A0119; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..551
FT /note="Solute carrier family 22 member 3"
FT /id="PRO_0000220505"
FT TRANSMEM 21..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 463..483
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 493..513
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 72
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 99
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 114
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 199
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 317
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 551 AA; 61050 MW; 23F0EBC24E4F3DE1 CRC64;
MPTFDQALRK AGEFGRFQRR VFLLLCLTGV TFAFLFVGVV FLGSQPDYYW CRGPRATALA
ERCAWSPEEE WNLTTPELHV PAERRGQGHC HRYLLEDTNT SSELSCDPLA AFPNRSAPLV
PCSGDWRYVE THSTIVSQFD LVCGNAWMLD LTQAILNLGF LAGAFTLGYA ADRYGRLIVY
LISCFGVGIT GVVVAFAPNF SVFVIFRFLQ GVFGKGAWMT CFVIVTEIVG SKQRRIVGIV
IQMFFTLGII ILPGIAYFTP SWQGIQLAIS LPSFLFLLYY WVVPESPRWL ITRKQGEKAL
QILRRVAKCN GKHLSSNYSE ITVTDEEVSN PSCLDLVRTP QMRKCTLILM FAWFTSAVVY
QGLVMRLGLI GGNLYMDFFI SGLVELPGAL LILLTIERLG RRLPFAASNI VAGVSCLVTA
FLPEGIPWLR TTVATLGRLG ITMAFEIVYL VNSELYPTTL RNFGVSLCSG LCDFGGIIAP
FLLFRLAAIW LELPLIIFGI LASVCGGLVM LLPETKGIAL PETVEDVEKL GSSQLHQCGR
KKKTQVSTSN V
