S22A4_MOUSE
ID S22A4_MOUSE Reviewed; 553 AA.
AC Q9Z306;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Solute carrier family 22 member 4;
DE AltName: Full=Organic cation/carnitine transporter 1;
GN Name=Slc22a4; Synonyms=Octn1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC STRAIN=C57BL/6J; TISSUE=Kidney;
RX PubMed=11010964; DOI=10.1074/jbc.m005340200;
RA Tamai I., Ohashi R., Nezu J., Sai Y., Kobayashi D., Oku A., Shimane M.,
RA Tsuji A.;
RT "Molecular and functional characterization of organic cation/carnitine
RT transporter family in mice.";
RL J. Biol. Chem. 275:40064-40072(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH PDZK1.
RX PubMed=14531806; DOI=10.1046/j.1523-1755.2003.00266.x;
RA Gisler S.M., Pribanic S., Bacic D., Forrer P., Gantenbein A.,
RA Sabourin L.A., Tsuji A., Zhao Z.-S., Manser E., Biber J., Murer H.;
RT "PDZK1: I. a major scaffolder in brush borders of proximal tubular cells.";
RL Kidney Int. 64:1733-1745(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Sodium-ion dependent, low affinity carnitine transporter.
CC Probably transports one sodium ion with one molecule of carnitine. Also
CC transports organic cations such as tetraethylammonium (TEA) without the
CC involvement of sodium. Relative uptake activity ratio of carnitine to
CC TEA is 1.78. {ECO:0000269|PubMed:11010964}.
CC -!- SUBUNIT: Interacts with PDZK1. {ECO:0000269|PubMed:14531806}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:11010964}; Multi-
CC pass membrane protein {ECO:0000269|PubMed:11010964}.
CC -!- TISSUE SPECIFICITY: Expressed in kidney, liver and testis. Weakly
CC expressed in other tissues. {ECO:0000269|PubMed:11010964}.
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
CC -!- CAUTION: It is unclear whether it transports carnitine in vivo.
CC {ECO:0000305}.
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DR EMBL; AB016257; BAA36626.1; -; mRNA.
DR EMBL; BC010590; AAH10590.1; -; mRNA.
DR CCDS; CCDS24689.1; -.
DR RefSeq; NP_062661.1; NM_019687.4.
DR AlphaFoldDB; Q9Z306; -.
DR SMR; Q9Z306; -.
DR STRING; 10090.ENSMUSP00000020586; -.
DR ChEMBL; CHEMBL2073694; -.
DR GlyGen; Q9Z306; 3 sites.
DR iPTMnet; Q9Z306; -.
DR PhosphoSitePlus; Q9Z306; -.
DR jPOST; Q9Z306; -.
DR PaxDb; Q9Z306; -.
DR PRIDE; Q9Z306; -.
DR ProteomicsDB; 253357; -.
DR Antibodypedia; 26020; 143 antibodies from 25 providers.
DR DNASU; 30805; -.
DR Ensembl; ENSMUST00000020586; ENSMUSP00000020586; ENSMUSG00000020334.
DR GeneID; 30805; -.
DR KEGG; mmu:30805; -.
DR UCSC; uc007ixe.1; mouse.
DR CTD; 6583; -.
DR MGI; MGI:1353479; Slc22a4.
DR VEuPathDB; HostDB:ENSMUSG00000020334; -.
DR eggNOG; KOG0255; Eukaryota.
DR GeneTree; ENSGT00940000154155; -.
DR HOGENOM; CLU_001265_33_4_1; -.
DR InParanoid; Q9Z306; -.
DR OMA; AYFTAWL; -.
DR OrthoDB; 655566at2759; -.
DR PhylomeDB; Q9Z306; -.
DR TreeFam; TF315847; -.
DR Reactome; R-MMU-549127; Organic cation transport.
DR BioGRID-ORCS; 30805; 4 hits in 74 CRISPR screens.
DR ChiTaRS; Slc22a4; mouse.
DR PRO; PR:Q9Z306; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q9Z306; protein.
DR Bgee; ENSMUSG00000020334; Expressed in right kidney and 118 other tissues.
DR Genevisible; Q9Z306; MM.
DR GO; GO:0016324; C:apical plasma membrane; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; IDA:MGI.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; ISO:MGI.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0015226; F:carnitine transmembrane transporter activity; IDA:MGI.
DR GO; GO:0015491; F:cation:cation antiporter activity; ISO:MGI.
DR GO; GO:0015101; F:organic cation transmembrane transporter activity; ISO:MGI.
DR GO; GO:0030165; F:PDZ domain binding; ISO:MGI.
DR GO; GO:0015651; F:quaternary ammonium group transmembrane transporter activity; ISO:MGI.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0089718; P:amino acid import across plasma membrane; ISO:MGI.
DR GO; GO:0009437; P:carnitine metabolic process; IGI:MGI.
DR GO; GO:0015879; P:carnitine transport; IDA:MGI.
DR GO; GO:0015697; P:quaternary ammonium group transport; IDA:MGI.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR GO; GO:0006641; P:triglyceride metabolic process; IGI:MGI.
DR GO; GO:0042908; P:xenobiotic transport; ISO:MGI.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004749; Orgcat_transp/SVOP.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00898; 2A0119; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Glycoprotein; Ion transport; Membrane; Nucleotide-binding;
KW Reference proteome; Sodium; Sodium transport; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..553
FT /note="Solute carrier family 22 member 4"
FT /id="PRO_0000220498"
FT TOPO_DOM 1..20
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 21..41
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 42..142
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 164..171
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 193..197
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 198..218
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 219..232
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 254..257
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..278
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 279..339
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 340..360
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 361..373
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 395..400
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 401..421
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 422..428
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 429..449
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 450..462
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 463..483
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 484..488
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 489..509
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 510..553
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT BINDING 218..225
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CARBOHYD 57
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 64
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 91
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 553 AA; 62290 MW; C4D66BC061398653 CRC64;
MRDYDEVIAF LGEWGPFQRL IFFLLSASII PNGFNGMSVV FLAGTPEHRC LVPDTVNLSS
SWRNHSIPLE TKDGRQVPQS CRRYRLATIA NFSAMGLEPG QDVDLEQLEQ ESCLDGWEYD
KDIFLSTIVT EWNLVCEDDW KTPLTTSLFF VGVLCGSFVS GQLSDRFGRK KVLFATMAVQ
TGFSFVQIFS TNWEMFTVLF AIVGMGQISN YVVAFILGTE ILSKSVRIIF STLGVCTFFA
IGYMVLPLFA YFIRDWRMLL LALTLPGLFC VPLWWFIPES PRWLISQRRF AEAEQIIQKA
AKMNSIVAPA GIFDPLELQE LNSLKQQKVI ILDLFRTRNI ATITVMAVML WMLTSVGYFA
LSLNVPNLHG DVYLNCFLSG LIEVPAYFTA WLLLRTLPRR YIIAGVLFWG GGVLLLIQVV
PEDYNFVSIG LVMLGKFGIT SAFSMLYVFT AELYPTLVRN MAVGITSMAS RVGSIIAPYF
VYLGAYNRLL PYILMGSLTV LIGIITLFFP ESFGVTLPEN LEQMQKVRGF RCGKKSTVSV
DREESPKVLI TAF
