S22A6_DANRE
ID S22A6_DANRE Reviewed; 560 AA.
AC Q6NYN7;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Solute carrier family 22 member 6;
DE AltName: Full=Organic anion transporter 1;
DE AltName: Full=Renal organic anion transporter 1;
DE Short=ROAT1;
GN Name=slc22a6; Synonyms=oat; ORFNames=zgc:77073;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the renal elimination of endogenous and exogenous
CC organic anions. Functions as organic anion exchanger when the uptake of
CC one molecule of organic anion is coupled with an efflux of one molecule
CC of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc).
CC Mediates the sodium-independent uptake of p-aminohippurate (PAH), 2,3-
CC dimercapto-1-propanesulfonic acid (DMPS), cidofovir, adefovir, 9-(2-
CC phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl)
CC diaminopurine (PMEDAP), ochratoxin (OTA), acyclovir (ACV), 3'-azido-
CC 3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate
CC (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and
CC 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF) and edaravone
CC sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate
CC (PCMBS), diethyl pyrocarbonate (DEPC), indomethacin, sulindac,
CC diclofenac, carprofen, okadaic acid, benzothiazolylcysteine (BTC), S-
CC chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-
CC dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate
CC 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin,
CC bumetamide, losartan, probenecid, phenol red, urate, glutarate and
CC alpha-ketoglutarate (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DOMAIN: Multiple cysteine residues are necessary for proper targeting
CC to the plasma membrane. {ECO:0000250}.
CC -!- PTM: Glycosylated. Glycosylation is necessary for proper targeting of
CC the transporter to the plasma membrane (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
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DR EMBL; BC066519; AAH66519.1; -; mRNA.
DR AlphaFoldDB; Q6NYN7; -.
DR SMR; Q6NYN7; -.
DR STRING; 7955.ENSDARP00000072353; -.
DR PaxDb; Q6NYN7; -.
DR ZFIN; ZDB-GENE-040426-2249; slc22a6l.
DR eggNOG; KOG0255; Eukaryota.
DR InParanoid; Q6NYN7; -.
DR PhylomeDB; Q6NYN7; -.
DR Reactome; R-DRE-561048; Organic anion transport.
DR PRO; PR:Q6NYN7; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:ZFIN.
DR GO; GO:0008514; F:organic anion transmembrane transporter activity; IDA:ZFIN.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..560
FT /note="Solute carrier family 22 member 6"
FT /id="PRO_0000324173"
FT TOPO_DOM 1..15
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..143
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 165..170
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 192..201
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 223..228
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 250..256
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 278..345
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 346..366
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 367..374
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 375..395
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 396..406
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..427
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 428..433
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 434..454
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 455..465
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 466..486
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 487..491
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 492..512
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 513..560
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 560 AA; 61972 MW; 14D3F32AB9771D45 CRC64;
MAFSDLLEQV GSTGRFQVLH VTLLSMPILM MASHNLLQNF VAAVPPHHCQ PHANLSMSST
DAVDVLRATV PLGLNGKLER CKRYTTPQWQ ILSLNTSESL WDESMAETET QSCEDGWFYN
MTEMSSTIIT EWDLVCDYRA LKQMSQTTYM GGVLVGAIVF GGLSDRFGRR VLLLISNLMM
AIGGTCVAFS TSFTMFCVFR VCCGMALSGL VLNSFSLIVE WIPTRVRTVV GTGTGYCYTT
GQLILAAVAY CIRDWRWLTL AVSLPFYVSF LYSWWFLESA RWLVLTKNPE QAVKNLKTVA
RINGRSAEGD KIDLEMLQES MKKEMACSKG SYSALDLLRT STMRTITICL SAVWFSTSFA
YYGLSMDLQK FGVSIYLIQI IFGAVDIPAK IIVTICMSML GRRPSQCGAL VLAGIMILIN
LLVPSDLQML RTSLAVIGKG CLAASFNCCY LYAGELYPTV IRQSGMGWVS MMARFGAMVA
PMVLLLGDDY PWIPGFIYGG APIVSGIFAF FLPETLSQPL PDTIQDIDDR GLARTNSKRL
PEKLDLAMKD PSCVLLKESV
