S22A8_HUMAN
ID S22A8_HUMAN Reviewed; 542 AA.
AC Q8TCC7; B4DPH7; F5GWA8; F5H5J1; O95820; Q59EW9; Q96TC1;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Solute carrier family 22 member 8;
DE AltName: Full=Organic anion transporter 3;
DE Short=hOAT3;
GN Name=SLC22A8; Synonyms=OAT3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC TISSUE=Kidney;
RX PubMed=10049739; DOI=10.1006/bbrc.1998.9978;
RA Race J.E., Grassl S.M., Williams W.J., Holtzman E.J.;
RT "Molecular cloning and characterization of two novel human renal organic
RT anion transporters (hOAT1 and hOAT3).";
RL Biochem. Biophys. Res. Commun. 255:508-514(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Kidney;
RA Cha S., Sekine T., Kanai Y., Endou H.;
RT "Molecular cloning and characterization of human organic anion transporter
RT 3.";
RL Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC TISSUE=Kidney;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Brain;
RA Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.,
RA Ohara O., Nagase T., Kikuno R.F.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=11912245; DOI=10.1681/asn.v134866;
RA Motohashi H., Sakurai Y., Saito H., Masuda S., Urakami Y., Goto M.,
RA Fukatsu A., Ogawa O., Inui K.;
RT "Gene expression levels and immunolocalization of organic ion transporters
RT in the human kidney.";
RL J. Am. Soc. Nephrol. 13:866-874(2002).
RN [8]
RP FUNCTION.
RX PubMed=14586168; DOI=10.1159/000074539;
RA Bakhiya A., Bahn A., Burckhardt G., Wolff N.;
RT "Human organic anion transporter 3 (hOAT3) can operate as an exchanger and
RT mediate secretory urate flux.";
RL Cell. Physiol. Biochem. 13:249-256(2003).
RN [9]
RP FUNCTION.
RX PubMed=15644426; DOI=10.1124/jpet.104.080366;
RA Srimaroeng C., Chatsudthipong V., Aslamkhan A.G., Pritchard J.B.;
RT "Transport of the natural sweetener stevioside and its aglycone steviol by
RT human organic anion transporter (hOAT1; SLC22A6) and hOAT3 (SLC22A8).";
RL J. Pharmacol. Exp. Ther. 313:621-628(2005).
RN [10]
RP FUNCTION.
RX PubMed=15846473; DOI=10.1007/s11095-005-2503-0;
RA Tahara H., Shono M., Kusuhara H., Kinoshita H., Fuse E., Takadate A.,
RA Otagiri M., Sugiyama Y.;
RT "Molecular cloning and functional analyses of OAT1 and OAT3 from cynomolgus
RT monkey kidney.";
RL Pharm. Res. 22:647-660(2005).
RN [11]
RP FUNCTION.
RX PubMed=16455804; DOI=10.1124/dmd.105.008375;
RA Tahara H., Kusuhara H., Maeda K., Koepsell H., Fuse E., Sugiyama Y.;
RT "Inhibition of oat3-mediated renal uptake as a mechanism for drug-drug
RT interaction between fexofenadine and probenecid.";
RL Drug Metab. Dispos. 34:743-747(2006).
RN [12]
RP VARIANT VAL-389.
RX PubMed=12811365; DOI=10.1016/s0009-9236(03)00060-2;
RA Nishizato Y., Ieiri I., Suzuki H., Kimura M., Kawabata K., Hirota T.,
RA Takane H., Irie S., Kusuhara H., Urasaki Y., Urae A., Higuchi S.,
RA Otsubo K., Sugiyama Y.;
RT "Polymorphisms of OATP-C (SLC21A6) and OAT3 (SLC22A8) genes: consequences
RT for pravastatin pharmacokinetics.";
RL Clin. Pharmacol. Ther. 73:554-565(2003).
RN [13]
RP VARIANTS SER-149; ARG-260; TRP-277 AND PHE-305, AND CHARACTERIZATION OF
RP VARIANTS SER-149; ARG-260; TRP-277 AND PHE-305.
RX PubMed=16291576; DOI=10.1152/ajprenal.00272.2005;
RA Erdman A.R., Mangravite L.M., Urban T.J., Lagpacan L.L., Castro R.A.,
RA de la Cruz M., Chan W., Huang C.C., Johns S.J., Kawamoto M., Stryke D.,
RA Taylor T.R., Carlson E.J., Ferrin T.E., Brett C.M., Burchard E.G.,
RA Giacomini K.M.;
RT "The human organic anion transporter 3 (OAT3; SLC22A8): genetic variation
RT and functional genomics.";
RL Am. J. Physiol. 290:F905-F912(2006).
CC -!- FUNCTION: Plays an important role in the excretion/detoxification of
CC endogenous and exogenous organic anions, especially from the brain and
CC kidney. Involved in the transport basolateral of steviol, fexofenadine.
CC Transports benzylpenicillin (PCG), estrone-3-sulfate (E1S), cimetidine
CC (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), p-amino-hippurate (PAH),
CC acyclovir (ACV) and ochratoxin (OTA). {ECO:0000269|PubMed:14586168,
CC ECO:0000269|PubMed:15644426, ECO:0000269|PubMed:15846473,
CC ECO:0000269|PubMed:16455804}.
CC -!- SUBCELLULAR LOCATION: Basolateral cell membrane {ECO:0000305}; Multi-
CC pass membrane protein {ECO:0000305}. Note=Localizes on the brush border
CC membrane of the choroid epithelial cells. Localizes to the basolateral
CC membrane of the proximal tubular cells. Localizes on the abluminal and
CC possibly, luminal membrane of the brain capillary endothelial cells
CC (BCEC) (By similarity). {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=1;
CC IsoId=Q8TCC7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8TCC7-2; Sequence=VSP_022565;
CC Name=5;
CC IsoId=Q8TCC7-5; Sequence=VSP_045824;
CC Name=3;
CC IsoId=Q8TCC7-3; Sequence=VSP_022562, VSP_022563, VSP_022564;
CC Name=4;
CC IsoId=Q8TCC7-4; Sequence=VSP_045272;
CC -!- TISSUE SPECIFICITY: Expressed in kidney. {ECO:0000269|PubMed:11912245}.
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD19357.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAD92929.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF097491; AAD19357.1; ALT_FRAME; mRNA.
DR EMBL; AB042505; BAB47393.1; -; mRNA.
DR EMBL; AK298341; BAG60589.1; -; mRNA.
DR EMBL; AB209692; BAD92929.1; ALT_INIT; mRNA.
DR EMBL; AP001858; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC022387; AAH22387.1; -; mRNA.
DR CCDS; CCDS53643.1; -. [Q8TCC7-5]
DR CCDS; CCDS53644.1; -. [Q8TCC7-4]
DR CCDS; CCDS8042.1; -. [Q8TCC7-1]
DR RefSeq; NP_001171661.1; NM_001184732.1. [Q8TCC7-1]
DR RefSeq; NP_001171662.1; NM_001184733.1. [Q8TCC7-4]
DR RefSeq; NP_001171665.1; NM_001184736.1. [Q8TCC7-5]
DR RefSeq; NP_004245.2; NM_004254.3. [Q8TCC7-1]
DR RefSeq; XP_011543666.1; XM_011545364.1. [Q8TCC7-5]
DR AlphaFoldDB; Q8TCC7; -.
DR SMR; Q8TCC7; -.
DR BioGRID; 114777; 22.
DR IntAct; Q8TCC7; 4.
DR STRING; 9606.ENSP00000337335; -.
DR BindingDB; Q8TCC7; -.
DR ChEMBL; CHEMBL1641348; -.
DR DrugBank; DB00945; Acetylsalicylic acid.
DR DrugBank; DB00787; Acyclovir.
DR DrugBank; DB00437; Allopurinol.
DR DrugBank; DB00345; Aminohippuric acid.
DR DrugBank; DB11901; Apalutamide.
DR DrugBank; DB09274; Artesunate.
DR DrugBank; DB00168; Aspartame.
DR DrugBank; DB05016; Ataluren.
DR DrugBank; DB11995; Avatrombopag.
DR DrugBank; DB09060; Avibactam.
DR DrugBank; DB11817; Baricitinib.
DR DrugBank; DB03793; Benzoic acid.
DR DrugBank; DB01053; Benzylpenicillin.
DR DrugBank; DB14669; Betamethasone phosphate.
DR DrugBank; DB12151; Brincidofovir.
DR DrugBank; DB01222; Budesonide.
DR DrugBank; DB00887; Bumetanide.
DR DrugBank; DB11751; Cabotegravir.
DR DrugBank; DB04519; Caprylic acid.
DR DrugBank; DB01414; Cefacetrile.
DR DrugBank; DB00833; Cefaclor.
DR DrugBank; DB01140; Cefadroxil.
DR DrugBank; DB09008; Cefaloridine.
DR DrugBank; DB00456; Cefalotin.
DR DrugBank; DB01326; Cefamandole.
DR DrugBank; DB01327; Cefazolin.
DR DrugBank; DB00535; Cefdinir.
DR DrugBank; DB01329; Cefoperazone.
DR DrugBank; DB00493; Cefotaxime.
DR DrugBank; DB00229; Cefotiam.
DR DrugBank; DB01415; Ceftibuten.
DR DrugBank; DB01332; Ceftizoxime.
DR DrugBank; DB01212; Ceftriaxone.
DR DrugBank; DB00567; Cephalexin.
DR DrugBank; DB02659; Cholic Acid.
DR DrugBank; DB01597; Cilastatin.
DR DrugBank; DB00501; Cimetidine.
DR DrugBank; DB01147; Cloxacillin.
DR DrugBank; DB00286; Conjugated estrogens.
DR DrugBank; DB01380; Cortisone acetate.
DR DrugBank; DB02527; Cyclic adenosine monophosphate.
DR DrugBank; DB08912; Dabrafenib.
DR DrugBank; DB04133; Degraded Cephaloridine.
DR DrugBank; DB01234; Dexamethasone.
DR DrugBank; DB14649; Dexamethasone acetate.
DR DrugBank; DB09213; Dexibuprofen.
DR DrugBank; DB00586; Diclofenac.
DR DrugBank; DB01160; Dinoprost tromethamine.
DR DrugBank; DB00917; Dinoprostone.
DR DrugBank; DB08930; Dolutegravir.
DR DrugBank; DB06211; Doripenem.
DR DrugBank; DB04855; Dronedarone.
DR DrugBank; DB12243; Edaravone.
DR DrugBank; DB09272; Eluxadoline.
DR DrugBank; DB09038; Empagliflozin.
DR DrugBank; DB00584; Enalapril.
DR DrugBank; DB13874; Enasidenib.
DR DrugBank; DB00736; Esomeprazole.
DR DrugBank; DB00783; Estradiol.
DR DrugBank; DB13952; Estradiol acetate.
DR DrugBank; DB13953; Estradiol benzoate.
DR DrugBank; DB13954; Estradiol cypionate.
DR DrugBank; DB13955; Estradiol dienanthate.
DR DrugBank; DB13956; Estradiol valerate.
DR DrugBank; DB00655; Estrone.
DR DrugBank; DB00927; Famotidine.
DR DrugBank; DB12466; Favipiravir.
DR DrugBank; DB12265; Fexinidazole.
DR DrugBank; DB00950; Fexofenadine.
DR DrugBank; DB00695; Furosemide.
DR DrugBank; DB01004; Ganciclovir.
DR DrugBank; DB01241; Gemfibrozil.
DR DrugBank; DB03553; Glutaric Acid.
DR DrugBank; DB00536; Guanidine.
DR DrugBank; DB00999; Hydrochlorothiazide.
DR DrugBank; DB00741; Hydrocortisone.
DR DrugBank; DB14538; Hydrocortisone aceponate.
DR DrugBank; DB14539; Hydrocortisone acetate.
DR DrugBank; DB14540; Hydrocortisone butyrate.
DR DrugBank; DB14541; Hydrocortisone cypionate.
DR DrugBank; DB14542; Hydrocortisone phosphate.
DR DrugBank; DB14543; Hydrocortisone probutate.
DR DrugBank; DB14544; Hydrocortisone valerate.
DR DrugBank; DB00774; Hydroflumethiazide.
DR DrugBank; DB01050; Ibuprofen.
DR DrugBank; DB00328; Indomethacin.
DR DrugBank; DB14568; Ivosidenib.
DR DrugBank; DB01009; Ketoprofen.
DR DrugBank; DB00448; Lansoprazole.
DR DrugBank; DB00654; Latanoprost.
DR DrugBank; DB09078; Lenvatinib.
DR DrugBank; DB12070; Letermovir.
DR DrugBank; DB00650; Leucovorin.
DR DrugBank; DB00583; Levocarnitine.
DR DrugBank; DB00601; Linezolid.
DR DrugBank; DB00279; Liothyronine.
DR DrugBank; DB01583; Liotrix.
DR DrugBank; DB00253; Medrysone.
DR DrugBank; DB01065; Melatonin.
DR DrugBank; DB01033; Mercaptopurine.
DR DrugBank; DB00563; Methotrexate.
DR DrugBank; DB06710; Methyltestosterone.
DR DrugBank; DB00788; Naproxen.
DR DrugBank; DB01051; Novobiocin.
DR DrugBank; DB00198; Oseltamivir.
DR DrugBank; DB11837; Osilodrostat.
DR DrugBank; DB01092; Ouabain.
DR DrugBank; DB03902; Oxalic Acid.
DR DrugBank; DB00595; Oxytetracycline.
DR DrugBank; DB00213; Pantoprazole.
DR DrugBank; DB00642; Pemetrexed.
DR DrugBank; DB00812; Phenylbutazone.
DR DrugBank; DB00319; Piperacillin.
DR DrugBank; DB00554; Piroxicam.
DR DrugBank; DB01324; Polythiazide.
DR DrugBank; DB12866; Pradigastat.
DR DrugBank; DB05804; Prasterone sulfate.
DR DrugBank; DB00175; Pravastatin.
DR DrugBank; DB14631; Prednisolone phosphate.
DR DrugBank; DB05154; Pretomanid.
DR DrugBank; DB01032; Probenecid.
DR DrugBank; DB00881; Quinapril.
DR DrugBank; DB00908; Quinidine.
DR DrugBank; DB00863; Ranitidine.
DR DrugBank; DB12377; Relebactam.
DR DrugBank; DB01045; Rifampicin.
DR DrugBank; DB12457; Rimegepant.
DR DrugBank; DB01098; Rosuvastatin.
DR DrugBank; DB12332; Rucaparib.
DR DrugBank; DB00936; Salicylic acid.
DR DrugBank; DB06335; Saxagliptin.
DR DrugBank; DB01261; Sitagliptin.
DR DrugBank; DB00139; Succinic acid.
DR DrugBank; DB11644; Tafamidis.
DR DrugBank; DB04348; Taurocholic acid.
DR DrugBank; DB01606; Tazobactam.
DR DrugBank; DB14126; Tenofovir.
DR DrugBank; DB09299; Tenofovir alafenamide.
DR DrugBank; DB00300; Tenofovir disoproxil.
DR DrugBank; DB00469; Tenoxicam.
DR DrugBank; DB00624; Testosterone.
DR DrugBank; DB13943; Testosterone cypionate.
DR DrugBank; DB13944; Testosterone enanthate.
DR DrugBank; DB13946; Testosterone undecanoate.
DR DrugBank; DB00759; Tetracycline.
DR DrugBank; DB08837; Tetraethylammonium.
DR DrugBank; DB01685; Topiroxostat.
DR DrugBank; DB01650; trans-2-hydroxycinnamic acid.
DR DrugBank; DB08844; Uric acid.
DR DrugBank; DB00577; Valaciclovir.
DR DrugBank; DB00313; Valproic acid.
DR DrugBank; DB00495; Zidovudine.
DR DrugCentral; Q8TCC7; -.
DR GuidetoPHARMACOLOGY; 1027; -.
DR TCDB; 2.A.1.19.34; the major facilitator superfamily (mfs).
DR GlyGen; Q8TCC7; 2 sites.
DR iPTMnet; Q8TCC7; -.
DR PhosphoSitePlus; Q8TCC7; -.
DR SwissPalm; Q8TCC7; -.
DR BioMuta; SLC22A8; -.
DR DMDM; 74730587; -.
DR jPOST; Q8TCC7; -.
DR MassIVE; Q8TCC7; -.
DR PaxDb; Q8TCC7; -.
DR PeptideAtlas; Q8TCC7; -.
DR PRIDE; Q8TCC7; -.
DR ProteomicsDB; 24057; -.
DR ProteomicsDB; 26892; -.
DR ProteomicsDB; 74117; -. [Q8TCC7-1]
DR ProteomicsDB; 74118; -. [Q8TCC7-2]
DR ProteomicsDB; 74119; -. [Q8TCC7-3]
DR Antibodypedia; 28883; 192 antibodies from 27 providers.
DR DNASU; 9376; -.
DR Ensembl; ENST00000336232.7; ENSP00000337335.2; ENSG00000149452.16. [Q8TCC7-1]
DR Ensembl; ENST00000430500.6; ENSP00000398548.2; ENSG00000149452.16. [Q8TCC7-1]
DR Ensembl; ENST00000535878.5; ENSP00000443368.1; ENSG00000149452.16. [Q8TCC7-5]
DR Ensembl; ENST00000545207.5; ENSP00000441658.1; ENSG00000149452.16. [Q8TCC7-4]
DR GeneID; 9376; -.
DR KEGG; hsa:9376; -.
DR MANE-Select; ENST00000336232.7; ENSP00000337335.2; NM_004254.4; NP_004245.2.
DR UCSC; uc001nwo.4; human. [Q8TCC7-1]
DR CTD; 9376; -.
DR DisGeNET; 9376; -.
DR GeneCards; SLC22A8; -.
DR HGNC; HGNC:10972; SLC22A8.
DR HPA; ENSG00000149452; Group enriched (choroid plexus, kidney).
DR MIM; 607581; gene.
DR neXtProt; NX_Q8TCC7; -.
DR OpenTargets; ENSG00000149452; -.
DR PharmGKB; PA389; -.
DR VEuPathDB; HostDB:ENSG00000149452; -.
DR eggNOG; KOG0255; Eukaryota.
DR GeneTree; ENSGT00940000154901; -.
DR HOGENOM; CLU_001265_33_3_1; -.
DR InParanoid; Q8TCC7; -.
DR OMA; RIPLQPC; -.
DR OrthoDB; 464838at2759; -.
DR PhylomeDB; Q8TCC7; -.
DR TreeFam; TF315847; -.
DR PathwayCommons; Q8TCC7; -.
DR Reactome; R-HSA-561048; Organic anion transport.
DR SignaLink; Q8TCC7; -.
DR BioGRID-ORCS; 9376; 14 hits in 1067 CRISPR screens.
DR GeneWiki; SLC22A8; -.
DR GenomeRNAi; 9376; -.
DR Pharos; Q8TCC7; Tclin.
DR PRO; PR:Q8TCC7; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q8TCC7; protein.
DR Bgee; ENSG00000149452; Expressed in pigmented layer of retina and 64 other tissues.
DR ExpressionAtlas; Q8TCC7; baseline and differential.
DR Genevisible; Q8TCC7; HS.
DR GO; GO:0016324; C:apical plasma membrane; IDA:ARUK-UCL.
DR GO; GO:0016323; C:basolateral plasma membrane; IDA:UniProtKB.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0015301; F:anion:anion antiporter activity; TAS:Reactome.
DR GO; GO:0005452; F:inorganic anion exchanger activity; IDA:UniProtKB.
DR GO; GO:0009636; P:response to toxic substance; IEA:UniProtKB-KW.
DR GO; GO:0150104; P:transport across blood-brain barrier; NAS:ARUK-UCL.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004749; Orgcat_transp/SVOP.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00898; 2A0119; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Detoxification; Glycoprotein;
KW Ion transport; Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..542
FT /note="Solute carrier family 22 member 8"
FT /id="PRO_0000273439"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..123
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..154
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 176
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 198..212
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 234..236
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..257
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 258..327
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 328..348
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 349..354
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 355..375
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 376..386
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 408..411
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 412..432
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 433..471
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 472..492
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 493..542
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 515..542
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 4
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9R1U7"
FT CARBOHYD 86
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 102
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..345
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_022562"
FT VAR_SEQ 1..123
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000305"
FT /id="VSP_045824"
FT VAR_SEQ 1..91
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_045272"
FT VAR_SEQ 406..455
FT /note="DLQTVRTVLAVFGKGCLSSSFSCLFLYTSELYPTVIRQTGMGVSNLWTRV
FT -> GERLGLPQNPLEEAARLGARDFTAGSASKSLCYLEQVPALSGAQGLSSRK (in
FT isoform 3)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_022563"
FT VAR_SEQ 456..542
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_022564"
FT VAR_SEQ 510
FT /note="W -> WSVTASGPPR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:10049739"
FT /id="VSP_022565"
FT VARIANT 129
FT /note="F -> L (in dbSNP:rs11568479)"
FT /id="VAR_030146"
FT VARIANT 149
FT /note="R -> S (complete loss of function;
FT dbSNP:rs45566039)"
FT /evidence="ECO:0000269|PubMed:16291576"
FT /id="VAR_030147"
FT VARIANT 260
FT /note="I -> R (complete loss of function;
FT dbSNP:rs11568493)"
FT /evidence="ECO:0000269|PubMed:16291576"
FT /id="VAR_030148"
FT VARIANT 277
FT /note="R -> W (reduced function; dbSNP:rs11568492)"
FT /evidence="ECO:0000269|PubMed:16291576"
FT /id="VAR_030149"
FT VARIANT 281
FT /note="V -> A (in 6% of African-Americans;
FT dbSNP:rs45438191)"
FT /id="VAR_030150"
FT VARIANT 305
FT /note="I -> F (in 3.5% of Asian-American; reduced function;
FT dbSNP:rs11568482)"
FT /evidence="ECO:0000269|PubMed:16291576"
FT /id="VAR_030151"
FT VARIANT 389
FT /note="A -> V (in dbSNP:rs749944923)"
FT /evidence="ECO:0000269|PubMed:12811365"
FT /id="VAR_030152"
FT VARIANT 448
FT /note="V -> I (in dbSNP:rs11568486)"
FT /id="VAR_030153"
FT CONFLICT 150
FT /note="Missing (in Ref. 1; AAD19357)"
FT /evidence="ECO:0000305"
FT CONFLICT 151
FT /note="P -> T (in Ref. 1; AAD19357)"
FT /evidence="ECO:0000305"
FT CONFLICT 271
FT /note="K -> E (in Ref. 2; BAB47393)"
FT /evidence="ECO:0000305"
FT CONFLICT 286
FT /note="Missing (in Ref. 1; AAD19357)"
FT /evidence="ECO:0000305"
FT CONFLICT 411
FT /note="R -> E (in Ref. 1; AAD19357)"
FT /evidence="ECO:0000305"
FT CONFLICT 478..480
FT /note="YGI -> FTGS (in Ref. 1; AAD19357)"
FT /evidence="ECO:0000305"
FT CONFLICT 526
FT /note="A -> T (in Ref. 3; BAG60589)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 542 AA; 59856 MW; 22799360D91B4CCF CRC64;
MTFSEILDRV GSMGHFQFLH VAILGLPILN MANHNLLQIF TAATPVHHCR PPHNASTGPW
VLPMGPNGKP ERCLRFVHPP NASLPNDTQR AMEPCLDGWV YNSTKDSIVT EWDLVCNSNK
LKEMAQSIFM AGILIGGLVL GDLSDRFGRR PILTCSYLLL AASGSGAAFS PTFPIYMVFR
FLCGFGISGI TLSTVILNVE WVPTRMRAIM STALGYCYTF GQFILPGLAY AIPQWRWLQL
TVSIPFFVFF LSSWWTPESI RWLVLSGKSS KALKILRRVA VFNGKKEEGE RLSLEELKLN
LQKEISLAKA KYTASDLFRI PMLRRMTFCL SLAWFATGFA YYSLAMGVEE FGVNLYILQI
IFGGVDVPAK FITILSLSYL GRHTTQAAAL LLAGGAILAL TFVPLDLQTV RTVLAVFGKG
CLSSSFSCLF LYTSELYPTV IRQTGMGVSN LWTRVGSMVS PLVKITGEVQ PFIPNIIYGI
TALLGGSAAL FLPETLNQPL PETIEDLENW SLRAKKPKQE PEVEKASQRI PLQPHGPGLG
SS
