S22AD_HUMAN
ID S22AD_HUMAN Reviewed; 551 AA.
AC Q9Y226; B2RCV9; Q8IYG1;
DT 04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2006, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Solute carrier family 22 member 13;
DE AltName: Full=Organic cation transporter-like 3;
DE Short=ORCTL-3;
GN Name=SLC22A13; Synonyms=OCTL1, ORCTL3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RX PubMed=10072596; DOI=10.1159/000015197;
RA Nishiwaki T., Daigo Y., Tamari M., Fujii Y., Nakamura Y.;
RT "Molecular cloning, mapping, and characterization of two novel human genes,
RT ORCTL3 and ORCTL4, bearing homology to organic-cation transporters.";
RL Cytogenet. Cell Genet. 83:251-255(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10231028; DOI=10.1093/dnares/6.1.37;
RA Daigo Y., Isomura M., Nishiwaki T., Tamari M., Ishikawa S., Kai M.,
RA Takeuchi K., Yamane Y., Hayashi R., Minami M., Fujino M.A., Hojo Y.,
RA Uchiyama I., Takagi T., Nakamura Y.;
RT "Characterization of a 1200-kb genomic segment of chromosome 3p22-p21.3.";
RL DNA Res. 6:37-44(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Kidney;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANTS ILE-43 AND
RP PHE-443.
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9Y226-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9Y226-2; Sequence=VSP_017830;
CC -!- TISSUE SPECIFICITY: Ubiquitous, expressed at low levels.
CC {ECO:0000269|PubMed:10072596}.
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB010438; BAA76350.1; -; mRNA.
DR EMBL; AB026898; BAA77625.1; -; Genomic_DNA.
DR EMBL; AK315301; BAG37706.1; -; mRNA.
DR EMBL; CH471055; EAW64527.1; -; Genomic_DNA.
DR EMBL; BC035973; AAH35973.1; -; mRNA.
DR CCDS; CCDS2676.1; -. [Q9Y226-1]
DR RefSeq; NP_004247.2; NM_004256.3. [Q9Y226-1]
DR AlphaFoldDB; Q9Y226; -.
DR SMR; Q9Y226; -.
DR BioGRID; 114790; 4.
DR IntAct; Q9Y226; 3.
DR STRING; 9606.ENSP00000310241; -.
DR TCDB; 2.A.1.19.17; the major facilitator superfamily (mfs).
DR GlyGen; Q9Y226; 5 sites, 1 O-linked glycan (1 site).
DR iPTMnet; Q9Y226; -.
DR PhosphoSitePlus; Q9Y226; -.
DR BioMuta; SLC22A13; -.
DR DMDM; 91207263; -.
DR MassIVE; Q9Y226; -.
DR PaxDb; Q9Y226; -.
DR PeptideAtlas; Q9Y226; -.
DR PRIDE; Q9Y226; -.
DR ProteomicsDB; 85612; -. [Q9Y226-1]
DR ProteomicsDB; 85613; -. [Q9Y226-2]
DR Antibodypedia; 28559; 73 antibodies from 19 providers.
DR DNASU; 9390; -.
DR Ensembl; ENST00000311856.9; ENSP00000310241.3; ENSG00000172940.13. [Q9Y226-1]
DR GeneID; 9390; -.
DR KEGG; hsa:9390; -.
DR MANE-Select; ENST00000311856.9; ENSP00000310241.3; NM_004256.4; NP_004247.2.
DR UCSC; uc003chz.4; human. [Q9Y226-1]
DR CTD; 9390; -.
DR DisGeNET; 9390; -.
DR GeneCards; SLC22A13; -.
DR HGNC; HGNC:8494; SLC22A13.
DR HPA; ENSG00000172940; Tissue enriched (kidney).
DR MIM; 604047; gene.
DR neXtProt; NX_Q9Y226; -.
DR OpenTargets; ENSG00000172940; -.
DR PharmGKB; PA32814; -.
DR VEuPathDB; HostDB:ENSG00000172940; -.
DR eggNOG; KOG0255; Eukaryota.
DR GeneTree; ENSGT00940000154607; -.
DR HOGENOM; CLU_001265_33_3_1; -.
DR InParanoid; Q9Y226; -.
DR OMA; YYMFAQV; -.
DR OrthoDB; 704438at2759; -.
DR PhylomeDB; Q9Y226; -.
DR TreeFam; TF315847; -.
DR PathwayCommons; Q9Y226; -.
DR Reactome; R-HSA-197264; Nicotinamide salvaging.
DR SignaLink; Q9Y226; -.
DR BioGRID-ORCS; 9390; 7 hits in 1058 CRISPR screens.
DR GenomeRNAi; 9390; -.
DR Pharos; Q9Y226; Tbio.
DR PRO; PR:Q9Y226; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q9Y226; protein.
DR Bgee; ENSG00000172940; Expressed in adult mammalian kidney and 37 other tissues.
DR ExpressionAtlas; Q9Y226; baseline and differential.
DR Genevisible; Q9Y226; HS.
DR GO; GO:0016324; C:apical plasma membrane; IDA:UniProtKB.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0090416; F:nicotinate transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0034356; P:NAD biosynthesis via nicotinamide riboside salvage pathway; TAS:Reactome.
DR GO; GO:0045922; P:negative regulation of fatty acid metabolic process; IDA:UniProtKB.
DR GO; GO:2001142; P:nicotinate transport; IDA:UniProtKB.
DR GO; GO:0002854; P:positive regulation of T cell mediated cytotoxicity directed against tumor cell target; IDA:UniProtKB.
DR GO; GO:0015747; P:urate transport; IDA:UniProtKB.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..551
FT /note="Solute carrier family 22 member 13"
FT /id="PRO_0000230782"
FT TOPO_DOM 1..20
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 21..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 42..138
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 160..167
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 189..195
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 217..224
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 246..251
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 273..332
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 333..353
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 354
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 355..375
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 376..397
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 398..418
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 419..427
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 428..448
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 449..452
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 453..473
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 474..478
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 479..499
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 500..551
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 511..551
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 57
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 61
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 92
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 104
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 451..551
FT /note="TGMGLVGIFSRIGGILTPLVILLGEYHAALPMLIYGSLPIVAGLLCTLLPET
FT HGQGLKDTLQDLELGPHPRSPKSVPSEKETEAKGRTSSPGVAFVSSTYF -> AWGWWA
FT SSHGSGASSHHL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_017830"
FT VARIANT 43
FT /note="V -> I (in dbSNP:rs17853496)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_025787"
FT VARIANT 443
FT /note="L -> F (in dbSNP:rs17857080)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_025788"
FT CONFLICT 146
FT /note="A -> G (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 551 AA; 60862 MW; 80D69A0D5DDDA447 CRC64;
MAQFVQVLAE IGDFGRFQIQ LLILLCVLNF LSPFYFFAHV FMVLDEPHHC AVAWVKNHTF
NLSAAEQLVL SVPLDTAGHP EPCLMFRPPP ANASLQDILS HRFNETQPCD MGWEYPENRL
PSLKNEFNLV CDRKHLKDTT QSVFMAGLLV GTLMFGPLCD RIGRKATILA QLLLFTLIGL
ATAFVPSFEL YMALRFAVAT AVAGLSFSNV TLLTEWVGPS WRTQAVVLAQ CNFSLGQMVL
AGLAYGFRNW RLLQITGTAP GLLLFFYFWA LPESARWLLT RGRMDEAIQL IQKAASVNRR
KLSPELMNQL VPEKTGPSGN ALDLFRHPQL RKVTLIIFCV WFVDSLGYYG LSLQVGDFGL
DVYLTQLIFG AVEVPARCSS IFMMQRFGRK WSQLGTLVLG GLMCIIIIFI PADLPVVVTM
LAVVGKMATA AAFTISYVYS AELFPTILRQ TGMGLVGIFS RIGGILTPLV ILLGEYHAAL
PMLIYGSLPI VAGLLCTLLP ETHGQGLKDT LQDLELGPHP RSPKSVPSEK ETEAKGRTSS
PGVAFVSSTY F
