S22AF_MOUSE
ID S22AF_MOUSE Reviewed; 544 AA.
AC Q504N2; A7MCW2; F8WJ00; Q8CDR3;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2013, sequence version 3.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Solute carrier family 22 member 15;
GN Name=Slc22a15;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 258-544 (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Probably transports organic cations. Appears not to be the
CC agmatine transporter. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q504N2-1; Sequence=Displayed;
CC Name=3;
CC IsoId=Q504N2-3; Sequence=VSP_034061, VSP_034064;
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
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DR EMBL; AK029695; BAC26569.1; -; mRNA.
DR EMBL; AC165165; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC152347; AAI52348.1; -; mRNA.
DR CCDS; CCDS51022.1; -. [Q504N2-1]
DR RefSeq; NP_001034460.2; NM_001039371.2. [Q504N2-1]
DR AlphaFoldDB; Q504N2; -.
DR SMR; Q504N2; -.
DR STRING; 10090.ENSMUSP00000102541; -.
DR GlyGen; Q504N2; 5 sites.
DR PhosphoSitePlus; Q504N2; -.
DR PaxDb; Q504N2; -.
DR PRIDE; Q504N2; -.
DR ProteomicsDB; 260759; -. [Q504N2-1]
DR ProteomicsDB; 260760; -. [Q504N2-3]
DR Antibodypedia; 9786; 73 antibodies from 19 providers.
DR DNASU; 242126; -.
DR Ensembl; ENSMUST00000106928; ENSMUSP00000102541; ENSMUSG00000033147. [Q504N2-1]
DR GeneID; 242126; -.
DR KEGG; mmu:242126; -.
DR UCSC; uc008qrl.2; mouse. [Q504N2-1]
DR CTD; 55356; -.
DR MGI; MGI:3607704; Slc22a15.
DR VEuPathDB; HostDB:ENSMUSG00000033147; -.
DR eggNOG; KOG0255; Eukaryota.
DR GeneTree; ENSGT00940000160364; -.
DR HOGENOM; CLU_001265_33_7_1; -.
DR InParanoid; Q504N2; -.
DR OMA; AGQFYPM; -.
DR OrthoDB; 464838at2759; -.
DR PhylomeDB; Q504N2; -.
DR TreeFam; TF315847; -.
DR Reactome; R-MMU-549127; Organic cation transport.
DR BioGRID-ORCS; 242126; 0 hits in 72 CRISPR screens.
DR PRO; PR:Q504N2; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q504N2; protein.
DR Bgee; ENSMUSG00000033147; Expressed in granulocyte and 155 other tissues.
DR ExpressionAtlas; Q504N2; baseline and differential.
DR Genevisible; Q504N2; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..544
FT /note="Solute carrier family 22 member 15"
FT /id="PRO_0000338620"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..358
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 368..388
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 401..420
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 433..453
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 462..482
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 513..544
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 58
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 63
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 80
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 106
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 286
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..311
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_034061"
FT VAR_SEQ 456..544
FT /note="KEVQWSLPFIVFGATGLTSGLLSLLLPETLNSPLLETFSDLQMYSYRRLGEE
FT ALSLQTLDPPQPLDKVSSESEEEEEFYDADEETQMIK -> VEGSTVVSALHRVWSHRP
FT DLRPPEPAIARDSEQPFAGDIF (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_034064"
FT CONFLICT 370..371
FT /note="AA -> PP (in Ref. 1; BAC26569)"
FT /evidence="ECO:0000305"
FT CONFLICT 457
FT /note="E -> D (in Ref. 3; AAI52348)"
FT /evidence="ECO:0000305"
FT CONFLICT 519
FT /note="P -> S (in Ref. 3; AAI52348)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 544 AA; 60286 MW; 8A58E1FB6B9EA80D CRC64;
MEVEEAFQAV GEMGLYQMYL CFLLAVLLQL YVATEAILIA LIGATPAYHW DMADLLPNQS
HSNQTLGKGQ AFGDWLLTAN GSEIHKHVHF SNSFTSIASE WFLIANRSYK VSAASSSFFS
GVFVGVISFG QLSDRFGRRK VYLTGFALDI LFAVANGFSP SYEFFAVTRF LVGMMNGGMS
LVAFVLLNEC VGTAYWALAG SIGGLFFAVG IAQYALLGYF IRSWRTLAVL VNLQGTLVFL
LSLFIPESPR WLYSQGRLSE AEEALYFIAK RNRKLKCTFS LTHPANRSYR ATGSFLDLFR
YRILLGHTLI LMFIWFVCSL VYYGLTLSAG DLGGSIYANL ALSGLIEIPS YPLCIYLINQ
RWFGRKRTLA AFLCLGGLAC LIVMFLPEKK DTGVFAVVNS HSLSLLGKLT ISAAFNIVYI
YTSELYPTVI RNVGLGACSM FSRVGGIIAP FVPSLKEVQW SLPFIVFGAT GLTSGLLSLL
LPETLNSPLL ETFSDLQMYS YRRLGEEALS LQTLDPPQPL DKVSSESEEE EEFYDADEET
QMIK
