S22AI_MOUSE
ID S22AI_MOUSE Reviewed; 406 AA.
AC Q78KK3; O35428; O54959; O88283; Q99NF4;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Solute carrier family 22 member 18;
DE AltName: Full=Imprinted multi-membrane-spanning polyspecific transporter-related protein 1;
DE Short=Multi-membrane-spanning polyspecific transporter;
DE AltName: Full=Organic cation transporter-like protein 2;
DE Short=ORCTL-2;
GN Name=Slc22a18; Synonyms=Impt1, Itm, Orctl2, Tssc5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=9802569; DOI=10.1093/dnares/5.4.235;
RA Morisaki H., Hatada I., Morisaki T., Mukai T.;
RT "A novel gene, ITM, located between p57KIP2 and IPL, is imprinted in
RT mice.";
RL DNA Res. 5:235-240(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=9570947; DOI=10.1006/geno.1998.5221;
RA Cooper P.R., Smilinich N.J., Day C.D., Nowak N.J., Reid L.H.,
RA Pearsall R.S., Reece M., Prawitt D., Landers J., Housman D.E.,
RA Winterpacht A., Zabel B.U., Pelletier J., Weissman B.E., Shows T.B.,
RA Higgins M.J.;
RT "Divergently transcribed overlapping genes expressed in liver and kidney
RT and located in the 11p15.5 imprinted domain.";
RL Genomics 49:38-51(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=9499412; DOI=10.1093/hmg/7.4.597;
RA Dao D., Frank D., Qian N., O'Keefe D., Vosatka R.J., Walsh C.P., Tycko B.;
RT "IMPT1, an imprinted gene similar to polyspecific transporter and multi-
RT drug resistance genes.";
RL Hum. Mol. Genet. 7:597-608(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv;
RX PubMed=11063728; DOI=10.1093/hmg/9.18.2691;
RA Engemann S., Stroedicke M., Paulsen M., Franck O., Reinhardt R., Lane N.,
RA Reik W., Walter J.;
RT "Sequence and functional comparison in the Beckwith-Wiedemann region:
RT implications for a novel imprinting centre and extended imprinting.";
RL Hum. Mol. Genet. 9:2691-2706(2000).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, and Liver;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May act as a transporter of organic cations based on a proton
CC efflux antiport mechanism. May play a role in the transport of
CC chloroquine and quinidine-related compounds in kidney (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with RNF167. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Apical cell membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}. Note=Localized at the apical membrane
CC surface of renal proximal tubules. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed at high levels in fetal and adult kidney
CC and liver, and extraembryonic membranes (yolk sac). Expressed at
CC moderate levels in intestine, heart, lung and testis.
CC {ECO:0000269|PubMed:9499412, ECO:0000269|PubMed:9570947,
CC ECO:0000269|PubMed:9802569}.
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC04788.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAH03734.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB012084; BAA32780.1; -; mRNA.
DR EMBL; AF037065; AAC04788.1; ALT_INIT; mRNA.
DR EMBL; AF028739; AAB82728.1; -; mRNA.
DR EMBL; AJ276505; CAC16401.2; -; Genomic_DNA.
DR EMBL; BC003734; AAH03734.2; ALT_INIT; mRNA.
DR RefSeq; NP_001036225.1; NM_001042760.1.
DR RefSeq; NP_032793.2; NM_008767.2.
DR AlphaFoldDB; Q78KK3; -.
DR SMR; Q78KK3; -.
DR STRING; 10090.ENSMUSP00000101537; -.
DR iPTMnet; Q78KK3; -.
DR PhosphoSitePlus; Q78KK3; -.
DR SwissPalm; Q78KK3; -.
DR jPOST; Q78KK3; -.
DR MaxQB; Q78KK3; -.
DR PaxDb; Q78KK3; -.
DR PRIDE; Q78KK3; -.
DR ProteomicsDB; 260761; -.
DR DNASU; 18400; -.
DR GeneID; 18400; -.
DR KEGG; mmu:18400; -.
DR UCSC; uc009kpg.1; mouse.
DR CTD; 5002; -.
DR MGI; MGI:1336884; Slc22a18.
DR eggNOG; ENOG502QT94; Eukaryota.
DR InParanoid; Q78KK3; -.
DR OrthoDB; 1090653at2759; -.
DR PhylomeDB; Q78KK3; -.
DR TreeFam; TF352510; -.
DR Reactome; R-MMU-549127; Organic cation transport.
DR BioGRID-ORCS; 18400; 4 hits in 73 CRISPR screens.
DR ChiTaRS; Slc22a18; mouse.
DR PRO; PR:Q78KK3; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q78KK3; protein.
DR GO; GO:0016324; C:apical plasma membrane; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:MGI.
DR GO; GO:0005635; C:nuclear envelope; ISO:MGI.
DR GO; GO:0008514; F:organic anion transmembrane transporter activity; TAS:MGI.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; ISO:MGI.
DR GO; GO:0006820; P:anion transport; TAS:MGI.
DR GO; GO:1990961; P:xenobiotic detoxification by transmembrane export across the plasma membrane; ISO:MGI.
DR GO; GO:0042908; P:xenobiotic transport; ISO:MGI.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR001958; Tet-R_TetA/multi-R_MdtG.
DR Pfam; PF07690; MFS_1; 1.
DR PRINTS; PR01035; TCRTETA.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Ion transport; Membrane; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..406
FT /note="Solute carrier family 22 member 18"
FT /id="PRO_0000220510"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 316..336
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 33
FT /note="Y -> V (in Ref. 4; CAC16401)"
FT /evidence="ECO:0000305"
FT CONFLICT 66
FT /note="F -> Y (in Ref. 4; CAC16401)"
FT /evidence="ECO:0000305"
FT CONFLICT 68
FT /note="D -> N (in Ref. 2; AAC04788)"
FT /evidence="ECO:0000305"
FT CONFLICT 83
FT /note="A -> G (in Ref. 3; AAB82728)"
FT /evidence="ECO:0000305"
FT CONFLICT 117
FT /note="L -> I (in Ref. 2; AAC04788)"
FT /evidence="ECO:0000305"
FT CONFLICT 119..120
FT /note="AA -> GT (in Ref. 4; CAC16401)"
FT /evidence="ECO:0000305"
FT CONFLICT 149
FT /note="I -> M (in Ref. 2; AAC04788)"
FT /evidence="ECO:0000305"
FT CONFLICT 164
FT /note="I -> Y (in Ref. 4; CAC16401)"
FT /evidence="ECO:0000305"
FT CONFLICT 178
FT /note="G -> R (in Ref. 3; AAB82728)"
FT /evidence="ECO:0000305"
FT CONFLICT 205
FT /note="A -> G (in Ref. 3; AAB82728)"
FT /evidence="ECO:0000305"
FT CONFLICT 228..231
FT /note="VKVI -> SVF (in Ref. 2; AAC04788)"
FT /evidence="ECO:0000305"
FT CONFLICT 238
FT /note="L -> V (in Ref. 4; CAC16401)"
FT /evidence="ECO:0000305"
FT CONFLICT 271
FT /note="M -> V (in Ref. 4; CAC16401)"
FT /evidence="ECO:0000305"
FT CONFLICT 305
FT /note="A -> V (in Ref. 4; CAC16401)"
FT /evidence="ECO:0000305"
FT CONFLICT 346
FT /note="T -> A (in Ref. 4; CAC16401)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 406 AA; 43013 MW; 034D8223CEB8013C CRC64;
MAALPRQGII ILTYVLAALE LTCLFMQFSI LPYLSRTLGL DSVSFGYLQT TFGVLQLLGG
PVFGRFADQC GARAALSLSF LAASALYLLL VASCSPALPG VFLLFASRIP SALMHTLPAA
QMVITDLTAP TERPAALGRL GLCFGIGIIF GSLLGGTLNT AYGIQCPAIL AFVVTLLGAV
LSFTCVPATT KEASVQSAPQ GGTKASVFDL KAITRLLLLP RVLPVFLVKV ISGLPSGLFL
VMFSIISMDF FQLEAAQAGY LMSFFGILQM MIQGLVIGRL STHFPEEALL RSSVLVFAVV
GLGMALMSSV LHFCFLMPGL VFSLCTLNVV TDSMLTKAVS ASDTGTMLGL SASVQPLTRT
LGPTLGGLLY RSYGVPIFGH VQLMVNLLVL LVLWKKPLSQ KGEKAR
