S22AK_HUMAN
ID S22AK_HUMAN Reviewed; 555 AA.
AC A6NK97; B9EJB2; Q6ZN88;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Solute carrier family 22 member 20;
DE AltName: Full=Organic anion transporter 6;
DE AltName: Full=Solute carrier family 22 member 20 pseudogene {ECO:0000312|HGNC:HGNC:29867};
GN Name=SLC22A20P {ECO:0000312|HGNC:HGNC:29867}; Synonyms=OAT6, SLC22A20;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Synovium;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Organic anion transporter that mediates the uptake of estrone
CC sulfate. Inhibited by probenecid, propionate, 2-methylbutyrate, 3-
CC methylbutyrate, benzoate, heptanoate and 2-ethylhaxanoate. May act as
CC an odorant transporter (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A6NK97-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A6NK97-2; Sequence=VSP_032653, VSP_032654;
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD18487.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK131327; BAD18487.1; ALT_INIT; mRNA.
DR EMBL; AP003068; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC146850; AAI46851.1; -; mRNA.
DR RefSeq; NP_001004326.4; NM_001004326.4.
DR AlphaFoldDB; A6NK97; -.
DR SMR; A6NK97; -.
DR BioGRID; 136241; 1.
DR IntAct; A6NK97; 2.
DR MINT; A6NK97; -.
DR TCDB; 2.A.1.19.35; the major facilitator superfamily (mfs).
DR GlyGen; A6NK97; 2 sites.
DR iPTMnet; A6NK97; -.
DR PhosphoSitePlus; A6NK97; -.
DR BioMuta; HGNC:29867; -.
DR jPOST; A6NK97; -.
DR PRIDE; A6NK97; -.
DR GeneCards; SLC22A20P; -.
DR HGNC; HGNC:29867; SLC22A20P.
DR MIM; 611696; gene.
DR neXtProt; NX_A6NK97; -.
DR InParanoid; A6NK97; -.
DR PhylomeDB; A6NK97; -.
DR PathwayCommons; A6NK97; -.
DR SignaLink; A6NK97; -.
DR BioGRID-ORCS; 440044; 13 hits in 193 CRISPR screens.
DR GenomeRNAi; 440044; -.
DR Pharos; A6NK97; Tdark.
DR PRO; PR:A6NK97; -.
DR Proteomes; UP000005640; Unplaced.
DR RNAct; A6NK97; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..555
FT /note="Solute carrier family 22 member 20"
FT /id="PRO_0000326428"
FT TOPO_DOM 1..23
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 24..44
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 45..137
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..166
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..194
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 216..225
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 247..250
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..271
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 272..339
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 340..360
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 361..373
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 395..397
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 398..418
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 419..429
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 430..450
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 451..485
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 486..506
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 507..555
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 526..555
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 526..542
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 54
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 114
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 213..347
FT /note="VEWMPTRGRTVAGILLGYSFTLGQLILAGVAYLIRPWRCLQFAISAPFLIFF
FT LYSWWLPESSRWLLLHGKSQLAVQNLQKVAAMNGRKEEGERLTKEVMSSYIQSEFASVC
FT TSNSILDLFRTPAIRKVTCCLMVI -> ASRVIPLAPPAWQVPVSCTESAEGGCNEREE
FT GGRGKADQGGDELLHPKRVCKCLHLQLNLGPLPNPGHPQGHMLSHGDLRGRQRSSGSGE
FT QGGSCEPESRNGRLPAVAPAGLGLPFVHLPLSAREKQILSSKFQS (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_032653"
FT VAR_SEQ 348..555
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_032654"
FT VARIANT 58
FT /note="A -> D (in dbSNP:rs11605576)"
FT /id="VAR_040065"
FT VARIANT 139
FT /note="A -> V (in dbSNP:rs12420456)"
FT /id="VAR_040066"
FT VARIANT A6NK97-2:244
FT /note="G -> G (in dbSNP:rs514076)"
FT /evidence="ECO:0000305"
FT /id="VAR_082917"
SQ SEQUENCE 555 AA; 60459 MW; 3F3D75A7FFB5E91C CRC64;
MAFTDLLDAL GSMGRFQLNH TALLLLPCGL LACHNFLQNF TAAVPPHHCR GPANHTEAST
NDSGAWLRAT IPLDQLGAPE PCRRFTKPQW ALLSPNSSIP GAATEGCKDG WVYNRSVFPS
TIVMEWDLVC EARTLRDLAQ SVYMAGVLVG AAVFGSLADR LGCKGPLVWS YLQLAASGAA
TAYFSSFSAY CVFRFLMGMT FSGIILNSVS LVVEWMPTRG RTVAGILLGY SFTLGQLILA
GVAYLIRPWR CLQFAISAPF LIFFLYSWWL PESSRWLLLH GKSQLAVQNL QKVAAMNGRK
EEGERLTKEV MSSYIQSEFA SVCTSNSILD LFRTPAIRKV TCCLMVIWFS NSVAYYGLAM
DLQKFGLSLY LVQALFGIIN TPAMLVATAT MIYVGRRATV ASFLILAGLM VIANMFVPEG
TQILCTAQAA LGKGCLASSF ICVYLFTGEL YPTEIRQMGM GFASVHARLG GLTAPLVTTL
GEYSTILPPV SFGATAILAG LAVCVLTETR NMPLVETIAA MERRVKEGSS KKHVEEKSEE
ISLQQLRASP LKETI
