S22AK_MOUSE
ID S22AK_MOUSE Reviewed; 556 AA.
AC Q80UJ1; Q5JBT1;
DT 07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Solute carrier family 22 member 20;
DE AltName: Full=Organic anion transporter 6;
GN Name=Slc22a20;
GN Synonyms=Gm963 {ECO:0000312|EMBL:AAH46588.1, ECO:0000312|MGI:MGI:2685809},
GN Oat6;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RA Mount D.B.;
RT "Sequence of murine OAT1-like organic anion transporter.";
RL Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000312|EMBL:AAH46588.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Olfactory epithelium {ECO:0000312|EMBL:AAH46588.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3] {ECO:0000305}
RP PUTATIVE FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=15369770; DOI=10.1016/j.bbrc.2004.08.112;
RA Monte J.C., Nagle M.A., Eraly S.A., Nigam S.K.;
RT "Identification of a novel murine organic anion transporter family member,
RT OAT6, expressed in olfactory mucosa.";
RL Biochem. Biophys. Res. Commun. 323:429-436(2004).
RN [4]
RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=16478971; DOI=10.1152/ajprenal.00497.2005;
RA Schnabolk G.W., Youngblood G.L., Sweet D.H.;
RT "Transport of estrone sulfate by the novel organic anion transporter Oat6
RT (Slc22a20).";
RL Am. J. Physiol. 291:F314-F321(2006).
RN [5]
RP TISSUE SPECIFICITY, AND FUNCTION.
RX PubMed=17094945; DOI=10.1016/j.bbrc.2006.10.136;
RA Kaler G., Truong D.M., Sweeney D.E., Logan D.W., Nagle M., Wu W.,
RA Eraly S.A., Nigam S.K.;
RT "Olfactory mucosa-expressed organic anion transporter, Oat6, manifests high
RT affinity interactions with odorant organic anions.";
RL Biochem. Biophys. Res. Commun. 351:872-876(2006).
RN [6]
RP BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=17553798; DOI=10.1074/jbc.m703467200;
RA Kaler G., Truong D.M., Khandelwal A., Nagle M., Eraly S.A., Swaan P.W.,
RA Nigam S.K.;
RT "Structural variation governs substrate specificity for organic anion
RT transporter (OAT) homologs. Potential remote sensing by OAT family
RT members.";
RL J. Biol. Chem. 282:23841-23853(2007).
RN [7]
RP BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=18174163; DOI=10.1074/jbc.m708615200;
RA Truong D.M., Kaler G., Khandelwal A., Swaan P.W., Nigam S.K.;
RT "Multi-level analysis of organic anion transporters 1, 3, and 6 reveals
RT major differences in structural determinants of antiviral discrimination.";
RL J. Biol. Chem. 283:8654-8663(2008).
CC -!- FUNCTION: Organic anion transporter that mediates the uptake of estrone
CC sulfate. Inhibited by probenecid, propionate, 2-methylbutyrate, 3-
CC methylbutyrate, benzoate, heptanoate and 2-ethylhaxanoate. May act as
CC an odorant transporter. {ECO:0000269|PubMed:16478971,
CC ECO:0000269|PubMed:17094945}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=109.8 uM for estrone sulfate {ECO:0000269|PubMed:16478971,
CC ECO:0000269|PubMed:17553798, ECO:0000269|PubMed:18174163};
CC Vmax=40 pmol/h/mg enzyme for ES uptake {ECO:0000269|PubMed:16478971,
CC ECO:0000269|PubMed:17553798, ECO:0000269|PubMed:18174163};
CC Vmax=4.6 pmol/h/mg enzyme for PAH uptake
CC {ECO:0000269|PubMed:16478971, ECO:0000269|PubMed:17553798,
CC ECO:0000269|PubMed:18174163};
CC Vmax=0.9 pmol/h/mg enzyme for prostaglandin E2 uptake
CC {ECO:0000269|PubMed:16478971, ECO:0000269|PubMed:17553798,
CC ECO:0000269|PubMed:18174163};
CC Vmax=0.28 pmol/h/mg enzyme for ibuprofen uptake
CC {ECO:0000269|PubMed:16478971, ECO:0000269|PubMed:17553798,
CC ECO:0000269|PubMed:18174163};
CC Vmax=4.8 pmol/h/mg enzyme for ochratoxin A uptake
CC {ECO:0000269|PubMed:16478971, ECO:0000269|PubMed:17553798,
CC ECO:0000269|PubMed:18174163};
CC Vmax=5.1 pmol/h/mg enzyme for fluorescein uptake
CC {ECO:0000269|PubMed:16478971, ECO:0000269|PubMed:17553798,
CC ECO:0000269|PubMed:18174163};
CC Vmax=2.1 pmol/h/mg enzyme for 2-carboxyfluorescein-DM uptake
CC {ECO:0000269|PubMed:16478971, ECO:0000269|PubMed:17553798,
CC ECO:0000269|PubMed:18174163};
CC Vmax=3.9 pmol/h/mg enzyme for eosin-Y uptake
CC {ECO:0000269|PubMed:16478971, ECO:0000269|PubMed:17553798,
CC ECO:0000269|PubMed:18174163};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Highly expressed in olfactory mucosa. Weakly
CC expressed in testis. Not detected in heart, spleen, lung, kidney or
CC brain. {ECO:0000269|PubMed:15369770, ECO:0000269|PubMed:17094945}.
CC -!- DEVELOPMENTAL STAGE: Expressed at embryonic day 7, but not later in
CC embryogenesis. Expressed in adult. {ECO:0000269|PubMed:15369770}.
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
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DR EMBL; AY194118; AAO91751.1; -; mRNA.
DR EMBL; BC046588; AAH46588.1; -; mRNA.
DR CCDS; CCDS29486.1; -.
DR RefSeq; NP_941052.1; NM_198650.2.
DR AlphaFoldDB; Q80UJ1; -.
DR SMR; Q80UJ1; -.
DR STRING; 10090.ENSMUSP00000049473; -.
DR BindingDB; Q80UJ1; -.
DR ChEMBL; CHEMBL5269; -.
DR DrugCentral; Q80UJ1; -.
DR TCDB; 2.A.1.19.16; the major facilitator superfamily (mfs).
DR GlyGen; Q80UJ1; 4 sites.
DR iPTMnet; Q80UJ1; -.
DR PhosphoSitePlus; Q80UJ1; -.
DR PaxDb; Q80UJ1; -.
DR PRIDE; Q80UJ1; -.
DR ProteomicsDB; 253361; -.
DR DNASU; 381203; -.
DR Ensembl; ENSMUST00000041827; ENSMUSP00000049473; ENSMUSG00000037451.
DR GeneID; 381203; -.
DR KEGG; mmu:381203; -.
DR UCSC; uc008ggh.1; mouse.
DR CTD; 381203; -.
DR MGI; MGI:2685809; Slc22a20.
DR VEuPathDB; HostDB:ENSMUSG00000037451; -.
DR eggNOG; KOG0255; Eukaryota.
DR GeneTree; ENSGT00940000162438; -.
DR HOGENOM; CLU_001265_33_3_1; -.
DR InParanoid; Q80UJ1; -.
DR OMA; QAFFYNA; -.
DR OrthoDB; 704438at2759; -.
DR PhylomeDB; Q80UJ1; -.
DR TreeFam; TF315847; -.
DR BioGRID-ORCS; 381203; 1 hit in 70 CRISPR screens.
DR PRO; PR:Q80UJ1; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q80UJ1; protein.
DR Bgee; ENSMUSG00000037451; Expressed in granulocyte and 3 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008514; F:organic anion transmembrane transporter activity; NAS:UniProtKB.
DR GO; GO:0015711; P:organic anion transport; NAS:UniProtKB.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..556
FT /note="Solute carrier family 22 member 20"
FT /id="PRO_0000220512"
FT TOPO_DOM 1..15
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..137
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..166
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..194
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 216..225
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 247..250
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..271
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 272..339
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 340..360
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 361..366
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 367..387
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 388..397
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 398..418
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 419..425
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 426..446
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 447..457
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 458..478
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 479..485
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 486..506
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 507..556
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 526..556
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 526..540
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 541..556
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 39
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 54
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 61
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 96
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 556 AA; 60944 MW; B0A89E00824AFC39 CRC64;
MAFTDLLDAL GGVGRFQLVY TALLLLPCGL LACHTFLQNF TAAAPPHHCQ HPANYTEPTT
NVSGVWLRAA IPLNQHGDPE PCRRYVEPQW ALLKPNASSH GVATEGCKDG WVYDRSIFPS
TIVMEWDLVC EARTLRDLAQ SIYMSGVLVG AALFGGLADR LGRKAPLVWS YLQLAVSGAA
TAYVGSFSAY CVFRFLMGMT FSGIILNSLS LVVEWMPTRG RTVAGILLGF SFTLGQLILA
GVAYLIRPWR WLQFAVSAPF LVFFLYSWWL PESSRWLLLH GKAQQAVQNL QKVAMMNGRK
AEGERLTTEV VSSYIQDEFA SVRTSNSILD LFRTPAIRRV TCCLMGVWFS NSVAYYGLAM
DLQKFGLSIY LVQALFGIID IPAMLVATTT MIYVGRRATV SSFLILAGLM VIANMFMPED
LQTLRTVQAA LGKGCLASSF ICVYLFTGEL YPTEIRQMGM GFASVNARLG GLVAPLITTL
GEISPVLPPV SFGATSVLAG MAVACFLTET RNVPLVETIA AMERRVKQGR SKRDTEQKSE
EISLQQLGAS PLKETI
