S22AP_HUMAN
ID S22AP_HUMAN Reviewed; 547 AA.
AC Q6T423; A4FU27;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Solute carrier family 22 member 25;
DE AltName: Full=Organic anion transporter UST6;
GN Name=SLC22A25 {ECO:0000312|HGNC:HGNC:32935};
GN Synonyms=UST6 {ECO:0000303|PubMed:15054140};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAR84082.1}
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND VARIANT VAL-486.
RC TISSUE=Liver {ECO:0000269|PubMed:15054140};
RX PubMed=15054140; DOI=10.1152/physiolgenomics.00014.2004;
RA Eraly S.A., Monte J.C., Nigam S.K.;
RT "Novel slc22 transporter homologs in fly, worm, and human clarify the
RT phylogeny of organic anion and cation transporters.";
RL Physiol. Genomics 18:12-24(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAI01317.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS GLY-250; THR-300 AND
RP VAL-486.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed exclusively in liver in both embryo and
CC adult. {ECO:0000269|PubMed:15054140}.
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
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DR EMBL; AY437532; AAR84082.1; -; mRNA.
DR EMBL; AP001880; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP003420; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC101316; AAI01317.1; -; mRNA.
DR CCDS; CCDS31592.1; -.
DR RefSeq; NP_955384.3; NM_199352.3.
DR RefSeq; XP_016873176.1; XM_017017687.1.
DR AlphaFoldDB; Q6T423; -.
DR SMR; Q6T423; -.
DR BioGRID; 132365; 2.
DR STRING; 9606.ENSP00000307443; -.
DR TCDB; 2.A.1.19.33; the major facilitator superfamily (mfs).
DR GlyGen; Q6T423; 2 sites.
DR iPTMnet; Q6T423; -.
DR PhosphoSitePlus; Q6T423; -.
DR BioMuta; SLC22A25; -.
DR DMDM; 296452891; -.
DR MassIVE; Q6T423; -.
DR PaxDb; Q6T423; -.
DR PeptideAtlas; Q6T423; -.
DR PRIDE; Q6T423; -.
DR Antibodypedia; 51271; 47 antibodies from 11 providers.
DR DNASU; 387601; -.
DR Ensembl; ENST00000306494.11; ENSP00000307443.6; ENSG00000196600.13.
DR GeneID; 387601; -.
DR KEGG; hsa:387601; -.
DR MANE-Select; ENST00000306494.11; ENSP00000307443.6; NM_199352.6; NP_955384.3.
DR UCSC; uc001nwr.2; human.
DR CTD; 387601; -.
DR DisGeNET; 387601; -.
DR GeneCards; SLC22A25; -.
DR HGNC; HGNC:32935; SLC22A25.
DR HPA; ENSG00000196600; Tissue enriched (liver).
DR MIM; 610792; gene.
DR neXtProt; NX_Q6T423; -.
DR OpenTargets; ENSG00000196600; -.
DR PharmGKB; PA162403567; -.
DR VEuPathDB; HostDB:ENSG00000196600; -.
DR eggNOG; KOG0255; Eukaryota.
DR GeneTree; ENSGT00940000167143; -.
DR HOGENOM; CLU_001265_33_3_1; -.
DR InParanoid; Q6T423; -.
DR OMA; APWALNH; -.
DR OrthoDB; 464838at2759; -.
DR PhylomeDB; Q6T423; -.
DR TreeFam; TF315847; -.
DR PathwayCommons; Q6T423; -.
DR BioGRID-ORCS; 387601; 37 hits in 1063 CRISPR screens.
DR ChiTaRS; SLC22A25; human.
DR GeneWiki; SLC22A25; -.
DR GenomeRNAi; 387601; -.
DR Pharos; Q6T423; Tdark.
DR PRO; PR:Q6T423; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q6T423; protein.
DR Bgee; ENSG00000196600; Expressed in right lobe of liver and 27 other tissues.
DR ExpressionAtlas; Q6T423; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015711; P:organic anion transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..547
FT /note="Solute carrier family 22 member 25"
FT /id="PRO_0000328926"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..145
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 167..177
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 199..204
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 205..225
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 226..234
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 235..255
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 256..259
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 281..349
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 350..370
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 371..377
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 378..398
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 399..406
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..427
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 428..434
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 435..455
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 456..470
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 471..491
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 492..494
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 495..515
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 516..547
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 56
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 102
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 89
FT /note="R -> C (in dbSNP:rs35722529)"
FT /id="VAR_057783"
FT VARIANT 250
FT /note="S -> G (in dbSNP:rs11231409)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_042576"
FT VARIANT 300
FT /note="R -> T (in dbSNP:rs11231397)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_042577"
FT VARIANT 448
FT /note="G -> A (in dbSNP:rs17157907)"
FT /id="VAR_057784"
FT VARIANT 486
FT /note="M -> V (in dbSNP:rs6591771)"
FT /evidence="ECO:0000269|PubMed:15054140,
FT ECO:0000269|PubMed:15489334"
FT /id="VAR_060287"
SQ SEQUENCE 547 AA; 61008 MW; A6964B2363FE68A0 CRC64;
MAFQDLLDQV GGLGRFQILQ MVFLIMFNVI VYHQTQLENF AAFILDHRCW VHILDNDTIP
DNDPGTLSQD ALLRISIPFD SNLRPEKCRR FVHPQWKLIH LNGTFPNTSE PDTEPCVDGW
VYDQSSFPST IVTKWDLVCE SQPLNSVAKF LFMAGMMVGG NLYGHLSDRF GRKFVLRWSY
LQLAIVGTCA AFAPTILVYC SLRFLAGAAT FSIIVNTVLL IVEWITHQFC AMALTLTLCA
ASIGHITLGS LAFVIRDQCI LQLVMSAPCF VFFLFSRWLA ESARWLIINN KPEEGLKELR
KAAHRNGMKN AEDILTMEVL KSTMKQELEA AQKKHSLCEL LRIPNICKRI CFLSFVRFAS
TIPFWGLTLH LQHLGNNVFL LQTLFGAVTL LANCVAPWAL NHMSRRLSQM LLMFLLATCL
LAIIFVPQEM QTLRVVLATL GVGAASLGIT CSTAQENELI PSIIRGRATG ITGNFANIGG
ALASLMMILS IYSRPLPWII YGVFAILSGL VVLLLPETRN QPLLDSIQDV ENEGVNSLAA
PQRSSVL
