S22AR_MOUSE
ID S22AR_MOUSE Reviewed; 551 AA.
AC Q76M72; Q76M71;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Solute carrier family 22 member 27 {ECO:0000312|MGI:MGI:3042283};
DE AltName: Full=Organic anion transporter 9 {ECO:0000303|PubMed:20332632};
GN Name=Slc22a27 {ECO:0000312|MGI:MGI:3042283};
GN Synonyms=Oat9 {ECO:0000303|PubMed:20332632};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000312|EMBL:BAB85630.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, BIOPHYSICOCHEMICAL
RP PROPERTIES, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC TISSUE=Liver {ECO:0000312|EMBL:BAB85630.1, ECO:0000312|EMBL:BAB85631.1};
RX PubMed=20332632; DOI=10.1159/000303060;
RA Tsuchida H., Anzai N., Shin H.J., Wempe M.F., Jutabha P., Enomoto A.,
RA Cha S.H., Satoh T., Ishida M., Sakurai H., Endou H.;
RT "Identification of a novel organic anion transporter mediating carnitine
RT transport in mouse liver and kidney.";
RL Cell. Physiol. Biochem. 25:511-522(2010).
RN [2] {ECO:0000312|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: [Isoform 1]: Does not appear to have transporter activity.
CC {ECO:0000269|PubMed:20332632}.
CC -!- FUNCTION: [Isoform 2]: Sodium-independent organic anion transporter
CC which exhibits high specificity for L-carnitine. Can also transport
CC salicylic acid and the drug cimetidine. {ECO:0000269|PubMed:20332632}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=2.8 uM for L-carnitine (isoform 2) {ECO:0000269|PubMed:20332632};
CC KM=16.1 uM for cimetidine (isoform 2) {ECO:0000269|PubMed:20332632};
CC KM=175.5 uM for salicylic acid (isoform 2)
CC {ECO:0000269|PubMed:20332632};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20332632};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=L {ECO:0000303|PubMed:20332632};
CC IsoId=Q76M72-1; Sequence=Displayed;
CC Name=2; Synonyms=S {ECO:0000303|PubMed:20332632};
CC IsoId=Q76M72-2; Sequence=VSP_058421;
CC -!- TISSUE SPECIFICITY: Expressed in proximal kidney tubules, and in liver
CC hepatocytes (at protein level). {ECO:0000269|PubMed:20332632}.
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
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DR EMBL; AB056442; BAB85630.1; -; mRNA.
DR EMBL; AB056443; BAB85631.1; -; mRNA.
DR EMBL; AC115715; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC122119; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS50380.1; -. [Q76M72-1]
DR CCDS; CCDS89333.1; -. [Q76M72-2]
DR RefSeq; NP_599017.1; NM_134256.1. [Q76M72-1]
DR RefSeq; XP_006526791.1; XM_006526728.1. [Q76M72-1]
DR AlphaFoldDB; Q76M72; -.
DR SMR; Q76M72; -.
DR STRING; 10090.ENSMUSP00000093991; -.
DR TCDB; 2.A.1.19.40; the major facilitator superfamily (mfs).
DR GlyGen; Q76M72; 5 sites.
DR iPTMnet; Q76M72; -.
DR PhosphoSitePlus; Q76M72; -.
DR jPOST; Q76M72; -.
DR PaxDb; Q76M72; -.
DR PRIDE; Q76M72; -.
DR ProteomicsDB; 260890; -. [Q76M72-1]
DR ProteomicsDB; 260891; -. [Q76M72-2]
DR DNASU; 171405; -.
DR Ensembl; ENSMUST00000075619; ENSMUSP00000093991; ENSMUSG00000067656. [Q76M72-1]
DR Ensembl; ENSMUST00000182102; ENSMUSP00000138475; ENSMUSG00000067656. [Q76M72-2]
DR GeneID; 171405; -.
DR KEGG; mmu:171405; -.
DR UCSC; uc008gls.1; mouse. [Q76M72-1]
DR UCSC; uc012bhz.1; mouse.
DR CTD; 171405; -.
DR MGI; MGI:3042283; Slc22a27.
DR VEuPathDB; HostDB:ENSMUSG00000067656; -.
DR eggNOG; KOG0255; Eukaryota.
DR GeneTree; ENSGT00940000161239; -.
DR InParanoid; Q76M72; -.
DR OMA; ANCITLI; -.
DR OrthoDB; 464838at2759; -.
DR PhylomeDB; Q76M72; -.
DR TreeFam; TF315847; -.
DR BioGRID-ORCS; 171405; 0 hits in 39 CRISPR screens.
DR ChiTaRS; Slc22a27; mouse.
DR PRO; PR:Q76M72; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q76M72; protein.
DR Bgee; ENSMUSG00000067656; Expressed in proximal tubule and 6 other tissues.
DR GO; GO:0016323; C:basolateral plasma membrane; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015301; F:anion:anion antiporter activity; ISO:MGI.
DR GO; GO:0015636; F:short-chain fatty acid transmembrane transporter activity; ISO:MGI.
DR GO; GO:0015347; F:sodium-independent organic anion transmembrane transporter activity; ISO:MGI.
DR GO; GO:0015711; P:organic anion transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Glycoprotein; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..551
FT /note="Solute carrier family 22 member 27"
FT /id="PRO_0000436775"
FT TOPO_DOM 1..15
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..145
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 146..166
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 167..173
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 195..203
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..234
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 235..255
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 256..258
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 259..279
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 280..348
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 349..369
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 370..376
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 398..405
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 406..426
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 427..432
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 433..453
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 454..467
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 468..488
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 489..494
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 495..515
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 516..551
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT REGION 523..551
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 528..551
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 39
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 56
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 62
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 102
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 107
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 170..277
FT /note="Missing (in isoform 2)"
FT /id="VSP_058421"
SQ SEQUENCE 551 AA; 62045 MW; CA81960DC4C3393F CRC64;
MSFQELLNQV GSLGRFQILQ IVFLLLLNAI VVPHIAMENF TAAIPNHRCW VPILDNDTAS
DNGSRILSQD DLLRISIPLD SNLRPEKCRR FAQPQWHLLH LNGTFSNVSE PDTEPCVDGW
VYDRSNFLST IVTEWDLVCE SQALNSVTKF SFMIGLFIGG IICGHLSDRL GRKFILTCAL
LQFAITETCV AFAPSFFIYC SLRFLAGLSV EPILVNSHLL MLEWTSPKFL TMMAALLSCA
PNIGYMISAG LAFLFRIWHH LQLTMSVPIF FFLILTRWLS ESARWLIVTN KPQKGLKELR
KVAHMNGMKN SGDLTMEIVR TSMKAELEAA KTKPSLRDLF HTSILRKRIC VLSFMRLFFT
VSIFGLAVHL QHLSSNIILL QFLISALAIL VSVIGPFVLN HIGRRITYLV LMSLRGIFIL
IAVFVPQEMQ TLRIIMATLA EGISSLCVGV SRLHTNELLP TTLRATAVGV IGFFGNSGSF
LSPLFMLLAT YYANMPWIFY GGFSIFNAFT VFLLPETKNQ PLPDSTHDVG NDWKESRKGK
KEDPIIKVTR F
