S23A3_HUMAN
ID S23A3_HUMAN Reviewed; 610 AA.
AC Q6PIS1; B7Z512; Q2PYN6; Q96NA6;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Solute carrier family 23 member 3;
DE AltName: Full=HPC E2-binding protein 3;
DE AltName: Full=Na(+)/L-ascorbic acid transporter 3;
DE AltName: Full=Sodium-dependent vitamin C transporter 3;
GN Name=SLC23A3; Synonyms=E2BP3, SVCT3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1-225 (ISOFORM 1).
RC TISSUE=Kidney;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 108-225 (ISOFORM 1).
RA Cheng J., Lan X.-Y., Zhang L.-Y., Guo J.;
RT "Cloning and analysis of the cDNA of Hepatitis C virus E2-binding protein
RT 3.";
RL Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q6PIS1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6PIS1-2; Sequence=VSP_033970;
CC Name=3;
CC IsoId=Q6PIS1-5; Sequence=VSP_043670;
CC -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC 2.A.40) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABC17635.1; Type=Miscellaneous discrepancy; Note=Fragment of a potential novel isoform.; Evidence={ECO:0000305};
CC Sequence=BAB70999.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR EMBL; AK055730; BAB70999.1; ALT_SEQ; mRNA.
DR EMBL; AK298277; BAH12748.1; -; mRNA.
DR EMBL; AC020575; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC068946; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC097468; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC030243; AAH30243.1; -; mRNA.
DR EMBL; DQ294736; ABC17635.1; ALT_SEQ; mRNA.
DR CCDS; CCDS42819.1; -. [Q6PIS1-2]
DR CCDS; CCDS46517.1; -. [Q6PIS1-5]
DR CCDS; CCDS46518.1; -. [Q6PIS1-1]
DR RefSeq; NP_001138361.1; NM_001144889.1. [Q6PIS1-1]
DR RefSeq; NP_001138362.1; NM_001144890.1. [Q6PIS1-5]
DR RefSeq; NP_653313.3; NM_144712.4. [Q6PIS1-2]
DR AlphaFoldDB; Q6PIS1; -.
DR SMR; Q6PIS1; -.
DR BioGRID; 127365; 3.
DR IntAct; Q6PIS1; 2.
DR STRING; 9606.ENSP00000406546; -.
DR TCDB; 2.A.40.6.4; the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.
DR PhosphoSitePlus; Q6PIS1; -.
DR BioMuta; SLC23A3; -.
DR DMDM; 189046185; -.
DR MassIVE; Q6PIS1; -.
DR PaxDb; Q6PIS1; -.
DR PeptideAtlas; Q6PIS1; -.
DR PRIDE; Q6PIS1; -.
DR ProteomicsDB; 67173; -. [Q6PIS1-1]
DR ProteomicsDB; 67174; -. [Q6PIS1-2]
DR ProteomicsDB; 67175; -. [Q6PIS1-5]
DR Antibodypedia; 11409; 66 antibodies from 17 providers.
DR DNASU; 151295; -.
DR Ensembl; ENST00000295738.11; ENSP00000295738.7; ENSG00000213901.11. [Q6PIS1-2]
DR Ensembl; ENST00000409878.8; ENSP00000386473.4; ENSG00000213901.11. [Q6PIS1-1]
DR Ensembl; ENST00000455516.6; ENSP00000406546.2; ENSG00000213901.11. [Q6PIS1-5]
DR GeneID; 151295; -.
DR KEGG; hsa:151295; -.
DR MANE-Select; ENST00000409878.8; ENSP00000386473.4; NM_001144889.2; NP_001138361.1.
DR UCSC; uc010fwb.4; human. [Q6PIS1-1]
DR CTD; 151295; -.
DR DisGeNET; 151295; -.
DR GeneCards; SLC23A3; -.
DR HGNC; HGNC:20601; SLC23A3.
DR HPA; ENSG00000213901; Group enriched (intestine, kidney).
DR neXtProt; NX_Q6PIS1; -.
DR OpenTargets; ENSG00000213901; -.
DR PharmGKB; PA134932069; -.
DR VEuPathDB; HostDB:ENSG00000213901; -.
DR eggNOG; KOG1292; Eukaryota.
DR GeneTree; ENSGT00950000182953; -.
DR HOGENOM; CLU_017959_5_4_1; -.
DR InParanoid; Q6PIS1; -.
DR OMA; CKAFPCC; -.
DR OrthoDB; 387031at2759; -.
DR PhylomeDB; Q6PIS1; -.
DR TreeFam; TF313272; -.
DR PathwayCommons; Q6PIS1; -.
DR SignaLink; Q6PIS1; -.
DR BioGRID-ORCS; 151295; 13 hits in 1073 CRISPR screens.
DR ChiTaRS; SLC23A3; human.
DR GenomeRNAi; 151295; -.
DR Pharos; Q6PIS1; Tdark.
DR PRO; PR:Q6PIS1; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q6PIS1; protein.
DR Bgee; ENSG00000213901; Expressed in kidney epithelium and 128 other tissues.
DR ExpressionAtlas; Q6PIS1; baseline and differential.
DR Genevisible; Q6PIS1; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0098590; C:plasma membrane region; IEA:UniProt.
DR GO; GO:0015229; F:L-ascorbic acid transmembrane transporter activity; IEA:UniProt.
DR InterPro; IPR006043; NCS2.
DR InterPro; IPR029957; SLC23A3.
DR PANTHER; PTHR11119:SF57; PTHR11119:SF57; 1.
DR Pfam; PF00860; Xan_ur_permease; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..610
FT /note="Solute carrier family 23 member 3"
FT /id="PRO_0000337203"
FT TOPO_DOM 1..49
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 50..70
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 71..85
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 107..164
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 186..187
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 188..208
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 209..211
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 233..266
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 267..287
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 288..316
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 338..355
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 356..376
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 377..394
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..414
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 415..423
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 424..446
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 447..452
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 453..472
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 473..486
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 487..507
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 508..610
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 571..610
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 140
FT /note="S -> CEHRARARA (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_043670"
FT VAR_SEQ 226..388
FT /note="VILLMVVCSQHLGSCQFHVCPWRRASTSSTHTPLPVFRLLSVLIPVACVWIV
FT SAFVGFSVIPQELSAPTKAPWIWLPHPGEWNWPLLTPRALAAGISMALAASTSSLGCYA
FT LCGRLLHLPPPPPHACSRGLSLEGLGSVLAGLLGSPMGTASSFPNVGKVGLI -> TWA
FT PASFMCAPGGELQRHQLTLLSLSSGSFRNCLPPPRHHGFGCLT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_033970"
FT CONFLICT 179
FT /note="M -> V (in Ref. 4; ABC17635)"
FT /evidence="ECO:0000305"
FT CONFLICT 543
FT /note="Missing (in Ref. 3; AAH30243)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 610 AA; 64531 MW; 962A3E1CBA895D10 CRC64;
MSRSPLNPSQ LRSVGSQDAL APLPPPAPQN PSTHSWDPLC GSLPWGLSCL LALQHVLVMA
SLLCVSHLLL LCSLSPGGLS YSPSQLLASS FFSCGMSTIL QTWMGSRLPL VQAPSLEFLI
PALVLTSQKL PRAIQTPGNS SLMLHLCRGP SCHGLGHWNT SLQEVSGAVV VSGLLQGMMG
LLGSPGHVFP HCGPLVLAPS LVVAGLSAHR EVAQFCFTHW GLALLVILLM VVCSQHLGSC
QFHVCPWRRA STSSTHTPLP VFRLLSVLIP VACVWIVSAF VGFSVIPQEL SAPTKAPWIW
LPHPGEWNWP LLTPRALAAG ISMALAASTS SLGCYALCGR LLHLPPPPPH ACSRGLSLEG
LGSVLAGLLG SPMGTASSFP NVGKVGLIQA GSQQVAHLVG LLCVGLGLSP RLAQLLTTIP
LPVVGGVLGV TQAVVLSAGF SSFYLADIDS GRNIFIVGFS IFMALLLPRW FREAPVLFST
GWSPLDVLLH SLLTQPIFLA GLSGFLLENT IPGTQLERGL GQGLPSPFTA QEARMPQKPR
EKAAQVYRLP FPIQNLCPCI PQPLHCLCPL PEDPGDEEGG SSEPEEMADL LPGSGEPCPE
SSREGFRSQK
