BEND5_HUMAN
ID BEND5_HUMAN Reviewed; 421 AA.
AC Q7L4P6; D3DQ27; Q96A62; Q9HAI3;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=BEN domain-containing protein 5;
GN Name=BEND5; Synonyms=C1orf165;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Embryo;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=B-cell, and Muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-133, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C.,
RA Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [5]
RP FUNCTION, DOMAIN BEN, AND DNA-BINDING.
RX PubMed=23468431; DOI=10.1101/gad.213314.113;
RA Dai Q., Ren A., Westholm J.O., Serganov A.A., Patel D.J., Lai E.C.;
RT "The BEN domain is a novel sequence-specific DNA-binding domain conserved
RT in neural transcriptional repressors.";
RL Genes Dev. 27:602-614(2013).
RN [6]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-258, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25755297; DOI=10.1074/mcp.o114.044792;
RA Xiao Z., Chang J.G., Hendriks I.A., Sigurdsson J.O., Olsen J.V.,
RA Vertegaal A.C.;
RT "System-wide analysis of SUMOylation dynamics in response to replication
RT stress reveals novel small ubiquitin-like modified target proteins and
RT acceptor lysines relevant for genome stability.";
RL Mol. Cell. Proteomics 14:1419-1434(2015).
RN [7]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-258, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- FUNCTION: Acts as a transcriptional repressor (PubMed:23468431).
CC {ECO:0000269|PubMed:23468431}.
CC -!- INTERACTION:
CC Q7L4P6; P41223: BUD31; NbExp=3; IntAct=EBI-724373, EBI-3904603;
CC Q7L4P6; O15371: EIF3D; NbExp=3; IntAct=EBI-724373, EBI-353818;
CC Q7L4P6; Q3B820: FAM161A; NbExp=3; IntAct=EBI-724373, EBI-719941;
CC Q7L4P6; Q9H8Y8: GORASP2; NbExp=4; IntAct=EBI-724373, EBI-739467;
CC Q7L4P6; Q5T7P3: LCE1B; NbExp=3; IntAct=EBI-724373, EBI-10245913;
CC Q7L4P6; Q8TAP4-4: LMO3; NbExp=3; IntAct=EBI-724373, EBI-11742507;
CC Q7L4P6; Q96HA8: NTAQ1; NbExp=3; IntAct=EBI-724373, EBI-741158;
CC Q7L4P6; Q9BSJ6: PIMREG; NbExp=3; IntAct=EBI-724373, EBI-2568609;
CC Q7L4P6; O43741: PRKAB2; NbExp=4; IntAct=EBI-724373, EBI-1053424;
CC Q7L4P6; Q8N8N7: PTGR2; NbExp=3; IntAct=EBI-724373, EBI-17614618;
CC Q7L4P6; Q14D33: RTP5; NbExp=3; IntAct=EBI-724373, EBI-10217913;
CC Q7L4P6; Q96GM5: SMARCD1; NbExp=3; IntAct=EBI-724373, EBI-358489;
CC Q7L4P6; Q7KZS0: UBE2I; NbExp=3; IntAct=EBI-724373, EBI-10180829;
CC Q7L4P6; Q9Y2K6: USP20; NbExp=3; IntAct=EBI-724373, EBI-2511991;
CC Q7L4P6; Q9BRG1: VPS25; NbExp=3; IntAct=EBI-724373, EBI-741945;
CC Q7L4P6; A0A0S2Z6H0: ZGPAT; NbExp=3; IntAct=EBI-724373, EBI-16428984;
CC Q7L4P6; Q8N5A5: ZGPAT; NbExp=3; IntAct=EBI-724373, EBI-3439227;
CC Q7L4P6; Q8TAU3: ZNF417; NbExp=3; IntAct=EBI-724373, EBI-740727;
CC Q7L4P6; A0A0S2Z5X4: ZNF688; NbExp=3; IntAct=EBI-724373, EBI-16429014;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7L4P6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7L4P6-2; Sequence=VSP_022695;
CC -!- DOMAIN: The BEN domain mediates DNA-binding.
CC {ECO:0000269|PubMed:23468431}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH11804.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK021650; BAB13864.1; -; mRNA.
DR EMBL; CH471059; EAX06851.1; -; Genomic_DNA.
DR EMBL; BC007932; AAH07932.2; -; mRNA.
DR EMBL; BC011804; AAH11804.1; ALT_INIT; mRNA.
DR CCDS; CCDS552.2; -. [Q7L4P6-1]
DR RefSeq; NP_001289011.1; NM_001302082.1. [Q7L4P6-2]
DR RefSeq; NP_078879.2; NM_024603.3. [Q7L4P6-1]
DR RefSeq; XP_011540444.1; XM_011542142.2. [Q7L4P6-2]
DR RefSeq; XP_016857823.1; XM_017002334.1.
DR AlphaFoldDB; Q7L4P6; -.
DR SMR; Q7L4P6; -.
DR BioGRID; 122782; 29.
DR IntAct; Q7L4P6; 32.
DR MINT; Q7L4P6; -.
DR STRING; 9606.ENSP00000360899; -.
DR GlyGen; Q7L4P6; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q7L4P6; -.
DR PhosphoSitePlus; Q7L4P6; -.
DR BioMuta; BEND5; -.
DR DMDM; 74738577; -.
DR EPD; Q7L4P6; -.
DR MassIVE; Q7L4P6; -.
DR MaxQB; Q7L4P6; -.
DR PaxDb; Q7L4P6; -.
DR PeptideAtlas; Q7L4P6; -.
DR PRIDE; Q7L4P6; -.
DR ProteomicsDB; 68779; -. [Q7L4P6-1]
DR ProteomicsDB; 68780; -. [Q7L4P6-2]
DR Antibodypedia; 53655; 69 antibodies from 24 providers.
DR DNASU; 79656; -.
DR Ensembl; ENST00000371833.4; ENSP00000360899.3; ENSG00000162373.13. [Q7L4P6-1]
DR GeneID; 79656; -.
DR KEGG; hsa:79656; -.
DR MANE-Select; ENST00000371833.4; ENSP00000360899.3; NM_024603.4; NP_078879.2.
DR UCSC; uc001crx.5; human. [Q7L4P6-1]
DR CTD; 79656; -.
DR DisGeNET; 79656; -.
DR GeneCards; BEND5; -.
DR HGNC; HGNC:25668; BEND5.
DR HPA; ENSG00000162373; Low tissue specificity.
DR neXtProt; NX_Q7L4P6; -.
DR OpenTargets; ENSG00000162373; -.
DR PharmGKB; PA164716542; -.
DR VEuPathDB; HostDB:ENSG00000162373; -.
DR eggNOG; ENOG502QTVS; Eukaryota.
DR GeneTree; ENSGT00390000001724; -.
DR HOGENOM; CLU_739568_0_0_1; -.
DR InParanoid; Q7L4P6; -.
DR OMA; HNTCPEE; -.
DR PhylomeDB; Q7L4P6; -.
DR TreeFam; TF331898; -.
DR PathwayCommons; Q7L4P6; -.
DR SignaLink; Q7L4P6; -.
DR BioGRID-ORCS; 79656; 9 hits in 1065 CRISPR screens.
DR GenomeRNAi; 79656; -.
DR Pharos; Q7L4P6; Tdark.
DR PRO; PR:Q7L4P6; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q7L4P6; protein.
DR Bgee; ENSG00000162373; Expressed in ganglionic eminence and 130 other tissues.
DR ExpressionAtlas; Q7L4P6; baseline and differential.
DR Genevisible; Q7L4P6; HS.
DR GO; GO:0005794; C:Golgi apparatus; IEA:Ensembl.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR InterPro; IPR018379; BEN_domain.
DR InterPro; IPR040391; BEND5.
DR PANTHER; PTHR14628; PTHR14628; 1.
DR Pfam; PF10523; BEN; 1.
DR SMART; SM01025; BEN; 1.
DR PROSITE; PS51457; BEN; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Coiled coil; DNA-binding;
KW Isopeptide bond; Reference proteome; Repressor; Transcription;
KW Transcription regulation; Ubl conjugation.
FT CHAIN 1..421
FT /note="BEN domain-containing protein 5"
FT /id="PRO_0000274279"
FT DOMAIN 302..408
FT /note="BEN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00784"
FT COILED 180..243
FT /evidence="ECO:0000255"
FT MOD_RES 133
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:19608861"
FT CROSSLNK 258
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:25755297,
FT ECO:0007744|PubMed:28112733"
FT VAR_SEQ 1..169
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_022695"
FT CONFLICT 363
FT /note="H -> R (in Ref. 1; BAB13864)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 421 AA; 48182 MW; C2C552B932F42AAC CRC64;
MYAFVRFLED NVCYALPVSC VRDFSPRSRL DFDNQKVYAV YRGPEELGAG PESPPRAPRD
WGALLLHKAQ ILALAEDKSD LENSVMQKKI KIPKLSLNHV EEDGEVKDYG EEDLQLRHIK
RPEGRKPSEV AHKSIEAVVA RLEKQNGLSL GHSTCPEEVF VEASPGTEDM DSLEDAVVPR
ALYEELLRNY QQQQEEMRHL QQELERTRRQ LVQQAKKLKE YGALVSEMKE LRDLNRRLQD
VLLLRLGSGP AIDLEKVKSE CLEPEPELRS TFSEEANTSS YYPAPAPVMD KYILDNGKVH
LGSGIWVDEE KWHQLQVTQG DSKYTKNLAV MIWGTDVLKN RSVTGVATKK KKDAVPKPPL
SPHKLSIVRE CLYDRIAQET VDETEIAQRL SKVNKYICEK IMDINKSCKN EERREAKYNL
Q
