BEND6_MOUSE
ID BEND6_MOUSE Reviewed; 281 AA.
AC Q6PFX2; Q8BLC9; Q8BXN2;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=BEN domain-containing protein 6;
GN Name=Bend6;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Corpora quadrigemina, and Retina;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=23571214; DOI=10.1242/dev.087502;
RA Dai Q., Andreu-Agullo C., Insolera R., Wong L.C., Shi S.H., Lai E.C.;
RT "BEND6 is a nuclear antagonist of Notch signaling during self-renewal of
RT neural stem cells.";
RL Development 140:1892-1902(2013).
RN [4]
RP FUNCTION.
RX PubMed=25561495; DOI=10.1101/gad.252122.114;
RA Dai Q., Ren A., Westholm J.O., Duan H., Patel D.J., Lai E.C.;
RT "Common and distinct DNA-binding and regulatory activities of the BEN-solo
RT transcription factor family.";
RL Genes Dev. 29:48-62(2015).
CC -!- FUNCTION: Acts as a corepressor of recombining binding protein
CC suppressor hairless (RBPJ) and inhibits Notch signaling in neural stem
CC cells, thereby opposing their self-renewal and promoting neurogenesis
CC (PubMed:23571214, PubMed:25561495). {ECO:0000269|PubMed:23571214,
CC ECO:0000269|PubMed:25561495}.
CC -!- SUBUNIT: Interacts (via BEN domain) with RBPJ.
CC {ECO:0000250|UniProtKB:Q5SZJ8}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23571214}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q6PFX2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6PFX2-2; Sequence=VSP_026968, VSP_026969;
CC Name=3;
CC IsoId=Q6PFX2-3; Sequence=VSP_026967;
CC -!- DEVELOPMENTAL STAGE: Expressed in differentiating neurons in embryonic
CC neocortex (at protein level). {ECO:0000269|PubMed:23571214}.
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DR EMBL; AK044633; BAC32011.1; -; mRNA.
DR EMBL; AK045534; BAC32409.1; -; mRNA.
DR EMBL; BC057378; AAH57378.1; -; mRNA.
DR CCDS; CCDS35533.1; -. [Q6PFX2-1]
DR CCDS; CCDS78557.1; -. [Q6PFX2-3]
DR RefSeq; NP_001297413.1; NM_001310484.1. [Q6PFX2-3]
DR RefSeq; NP_796209.2; NM_177235.3. [Q6PFX2-1]
DR AlphaFoldDB; Q6PFX2; -.
DR SMR; Q6PFX2; -.
DR BioGRID; 236229; 1.
DR STRING; 10090.ENSMUSP00000052919; -.
DR iPTMnet; Q6PFX2; -.
DR PhosphoSitePlus; Q6PFX2; -.
DR PaxDb; Q6PFX2; -.
DR PRIDE; Q6PFX2; -.
DR ProteomicsDB; 273483; -. [Q6PFX2-1]
DR ProteomicsDB; 273484; -. [Q6PFX2-2]
DR ProteomicsDB; 273485; -. [Q6PFX2-3]
DR Antibodypedia; 31120; 84 antibodies from 18 providers.
DR DNASU; 320705; -.
DR Ensembl; ENSMUST00000062289; ENSMUSP00000052919; ENSMUSG00000042182. [Q6PFX2-1]
DR Ensembl; ENSMUST00000115161; ENSMUSP00000110814; ENSMUSG00000042182. [Q6PFX2-3]
DR GeneID; 320705; -.
DR KEGG; mmu:320705; -.
DR UCSC; uc007aoc.1; mouse. [Q6PFX2-1]
DR UCSC; uc007aoe.1; mouse. [Q6PFX2-2]
DR CTD; 221336; -.
DR MGI; MGI:2444572; Bend6.
DR VEuPathDB; HostDB:ENSMUSG00000042182; -.
DR eggNOG; ENOG502SDDU; Eukaryota.
DR GeneTree; ENSGT00390000016954; -.
DR HOGENOM; CLU_1506790_0_0_1; -.
DR InParanoid; Q6PFX2; -.
DR OMA; WRAANNS; -.
DR OrthoDB; 880625at2759; -.
DR PhylomeDB; Q6PFX2; -.
DR TreeFam; TF335394; -.
DR BioGRID-ORCS; 320705; 3 hits in 72 CRISPR screens.
DR PRO; PR:Q6PFX2; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q6PFX2; protein.
DR Bgee; ENSMUSG00000042182; Expressed in pontine nuclear group and 139 other tissues.
DR ExpressionAtlas; Q6PFX2; baseline and differential.
DR Genevisible; Q6PFX2; MM.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0003682; F:chromatin binding; IDA:MGI.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003714; F:transcription corepressor activity; IGI:MGI.
DR GO; GO:0045746; P:negative regulation of Notch signaling pathway; IDA:MGI.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; IMP:MGI.
DR InterPro; IPR018379; BEN_domain.
DR InterPro; IPR037496; BEND6.
DR PANTHER; PTHR35346; PTHR35346; 1.
DR Pfam; PF10523; BEN; 1.
DR SMART; SM01025; BEN; 1.
DR PROSITE; PS51457; BEN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Neurogenesis; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..281
FT /note="BEN domain-containing protein 6"
FT /id="PRO_0000295661"
FT DOMAIN 171..271
FT /note="BEN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00784"
FT REGION 15..62
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 143..172
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 19..99
FT /evidence="ECO:0000255"
FT COMPBIAS 143..163
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..62
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_026967"
FT VAR_SEQ 239..251
FT /note="VTKQVFPSADDVS -> DRLITLYFPMNMK (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_026968"
FT VAR_SEQ 252..281
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_026969"
SQ SEQUENCE 281 AA; 31270 MW; B392DF45D85DAF8A CRC64;
MQKILQTDDI TDNQVLRKRK RKRTETANSE NANSALEKAQ RDPYSGNAFL PGESSSDEET
PLMELSKEEL CNKIESLKEK LRSIRKENSR LRQSLVMLQV LPQAVTQFEE LVGMAETLLK
SGGAVSTPAS TLWRATNNSS PDSFASLCSN SNSTSSSPSS VKAEEEQHPG EKQFTIERWQ
IARCNKSKPQ KFINDLMQVL YTNEYMATHS LTGAKSSTSR DKVVKPAMNQ NEVQEIIGVT
KQVFPSADDV SIRRMIGQKL NNCTKKPNAS KAPNSQDGIL K
