S2540_MOUSE
ID S2540_MOUSE Reviewed; 337 AA.
AC Q8BGP6; Q3UVZ5; Q8BXT8;
DT 26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Probable mitochondrial glutathione transporter SLC25A40 {ECO:0000305};
DE AltName: Full=Solute carrier family 25 member 40 {ECO:0000305};
GN Name=Slc25a40 {ECO:0000303|PubMed:34707288, ECO:0000312|MGI:MGI:2442486};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD;
RC TISSUE=Corpora quadrigemina, Diencephalon, Heart, Retina, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Embryonic stem cell;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=34707288; DOI=10.1038/s41586-021-04025-w;
RA Wang Y., Yen F.S., Zhu X.G., Timson R.C., Weber R., Xing C., Liu Y.,
RA Allwein B., Luo H., Yeh H.W., Heissel S., Unlu G., Gamazon E.R.,
RA Kharas M.G., Hite R., Birsoy K.;
RT "SLC25A39 is necessary for mitochondrial glutathione import in mammalian
RT cells.";
RL Nature 599:136-140(2021).
CC -!- FUNCTION: Probable mitochondrial transporter required for glutathione
CC import into mitochondria. Glutathione, which plays key roles in
CC oxidative metabolism, is produced exclusively in the cytosol and is
CC imported in many organelles (By similarity). Mitochondrial glutathione
CC is required for the activity and stability of proteins containing iron-
CC sulfur clusters, as well as erythropoiesis (PubMed:34707288).
CC {ECO:0000250|UniProtKB:Q8TBP6, ECO:0000269|PubMed:34707288}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q9BZJ4}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BGP6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BGP6-2; Sequence=VSP_026243;
CC -!- DISRUPTION PHENOTYPE: Cells lacking both Slc25a39 and Slc25a40 show
CC defects in the activity and stability of proteins containing iron-
CC sulfur clusters. {ECO:0000269|PubMed:34707288}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK044260; BAC31842.1; -; mRNA.
DR EMBL; AK045863; BAC32513.1; -; mRNA.
DR EMBL; AK084797; BAC39281.1; -; mRNA.
DR EMBL; AK088444; BAC40356.1; -; mRNA.
DR EMBL; AK136770; BAE23124.1; -; mRNA.
DR EMBL; BC083103; AAH83103.1; -; mRNA.
DR CCDS; CCDS39010.1; -. [Q8BGP6-1]
DR RefSeq; NP_001276524.1; NM_001289595.1. [Q8BGP6-1]
DR RefSeq; NP_001276525.1; NM_001289596.1. [Q8BGP6-2]
DR RefSeq; NP_848881.2; NM_178766.5. [Q8BGP6-1]
DR RefSeq; XP_006503654.1; XM_006503591.3. [Q8BGP6-1]
DR AlphaFoldDB; Q8BGP6; -.
DR SMR; Q8BGP6; -.
DR BioGRID; 235425; 1.
DR STRING; 10090.ENSMUSP00000067611; -.
DR PhosphoSitePlus; Q8BGP6; -.
DR SwissPalm; Q8BGP6; -.
DR EPD; Q8BGP6; -.
DR jPOST; Q8BGP6; -.
DR MaxQB; Q8BGP6; -.
DR PaxDb; Q8BGP6; -.
DR PeptideAtlas; Q8BGP6; -.
DR PRIDE; Q8BGP6; -.
DR ProteomicsDB; 256864; -. [Q8BGP6-1]
DR ProteomicsDB; 256865; -. [Q8BGP6-2]
DR Antibodypedia; 29759; 42 antibodies from 11 providers.
DR DNASU; 319653; -.
DR Ensembl; ENSMUST00000066921; ENSMUSP00000067611; ENSMUSG00000054099. [Q8BGP6-1]
DR Ensembl; ENSMUST00000170496; ENSMUSP00000130630; ENSMUSG00000054099. [Q8BGP6-1]
DR GeneID; 319653; -.
DR KEGG; mmu:319653; -.
DR UCSC; uc008wkf.3; mouse. [Q8BGP6-1]
DR CTD; 55972; -.
DR MGI; MGI:2442486; Slc25a40.
DR VEuPathDB; HostDB:ENSMUSG00000054099; -.
DR eggNOG; KOG0761; Eukaryota.
DR GeneTree; ENSGT00940000160249; -.
DR HOGENOM; CLU_015166_0_0_1; -.
DR InParanoid; Q8BGP6; -.
DR OMA; YWWGYES; -.
DR OrthoDB; 1007936at2759; -.
DR PhylomeDB; Q8BGP6; -.
DR TreeFam; TF314720; -.
DR BioGRID-ORCS; 319653; 3 hits in 71 CRISPR screens.
DR PRO; PR:Q8BGP6; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8BGP6; protein.
DR Bgee; ENSMUSG00000054099; Expressed in paneth cell and 165 other tissues.
DR ExpressionAtlas; Q8BGP6; baseline and differential.
DR Genevisible; Q8BGP6; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0048821; P:erythrocyte development; IMP:UniProtKB.
DR GO; GO:1990542; P:mitochondrial transmembrane transport; IEA:InterPro.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR002067; Mit_carrier.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR InterPro; IPR045315; Mtm1-like.
DR PANTHER; PTHR45760; PTHR45760; 1.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00926; MITOCARRIER.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..337
FT /note="Probable mitochondrial glutathione transporter
FT SLC25A40"
FT /id="PRO_0000291810"
FT TRANSMEM 20..40
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..221
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..319
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 14..132
FT /note="Solcar 1"
FT REPEAT 140..224
FT /note="Solcar 2"
FT REPEAT 234..328
FT /note="Solcar 3"
FT VAR_SEQ 1..63
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_026243"
FT CONFLICT 24
FT /note="C -> Y (in Ref. 1; BAC31842)"
FT /evidence="ECO:0000305"
FT CONFLICT 156
FT /note="A -> G (in Ref. 1; BAE23124)"
FT /evidence="ECO:0000305"
FT CONFLICT 183
FT /note="F -> L (in Ref. 1; BAE23124)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 337 AA; 37964 MW; 80554124DD995581 CRC64;
MEPETEGPPP TIPVTPLQQM IASCTGAVLT SLMVTPLDVV KIRLQAQNNP FPKGKCFLYS
NGLMDHMCVC EEESKKAWYK KPGNFRGTLD AFLKILRNEG IKSLWSGLPP TLVMAIPATV
IYFTCYEQLS AFLKTKLGEN ETRIPIVAGV VARFGAVTVI SPLELIRTKV QSKKFSYKEL
YQFVSMRVSE DGWISLWKGW APTILRDVPF SAMYWYNYEN LKRWLCEKSG LYEPTFMINF
TSGALSGSFA AVATLPFDVV KTQKQTQLWT NEYCKFPAPL DMSTWTIMKN IVADKGFSGL
FTGLIPRLVK IVPACAIMIS SYELGKSFFQ KQNVESR
