S26A8_MACFA
ID S26A8_MACFA Reviewed; 727 AA.
AC Q4R445;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 25-MAY-2022, entry version 65.
DE RecName: Full=Testis anion transporter 1;
DE AltName: Full=Anion exchange transporter;
DE AltName: Full=Solute carrier family 26 member 8;
GN Name=SLC26A8 {ECO:0000312|EMBL:BAE02121.1}; ORFNames=QtsA-12484;
OS Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC Cercopithecidae; Cercopithecinae; Macaca.
OX NCBI_TaxID=9541;
RN [1] {ECO:0000312|EMBL:BAE02121.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis {ECO:0000269|Ref.1};
RG International consortium for macaque cDNA sequencing and analysis;
RT "DNA sequences of macaque genes expressed in brain or testis and its
RT evolutionary implications.";
RL Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Acts as a DIDS-sensitive anion exchanger mediating chloride,
CC sulfate and oxalate transport. May fulfill critical anion exchange
CC functions in male germ line during meiosis and hence may play a role in
CC spermatogenesis. May be involved in a new regulatory pathway linking
CC sulfate transport to RhoGTPase signaling in male germ cells. A critical
CC component of the sperm annulus that is essential for correct sperm tail
CC differentiation and motility and hence male fertility (By similarity).
CC {ECO:0000250|UniProtKB:Q8R0C3, ECO:0000250|UniProtKB:Q96RN1}.
CC -!- SUBUNIT: Interacts with RACGAP1. {ECO:0000250|UniProtKB:Q96RN1}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:Q8R0C3,
CC ECO:0000250|UniProtKB:Q96RN1}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q8R0C3, ECO:0000250|UniProtKB:Q96RN1}.
CC Note=Located at the annulus ring structure within the sperm cell.
CC {ECO:0000250|UniProtKB:Q8R0C3, ECO:0000250|UniProtKB:Q96RN1}.
CC -!- PTM: N-glycosylated. {ECO:0000250|UniProtKB:Q96RN1}.
CC -!- SIMILARITY: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.
CC {ECO:0000255}.
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DR EMBL; AB179070; BAE02121.1; -; mRNA.
DR AlphaFoldDB; Q4R445; -.
DR SMR; Q4R445; -.
DR STRING; 9541.XP_005553259.1; -.
DR eggNOG; KOG0236; Eukaryota.
DR Proteomes; UP000233100; Unplaced.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015301; F:anion:anion antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0008271; F:secondary active sulfate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0030317; P:flagellated sperm motility; IEA:InterPro.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0048240; P:sperm capacitation; IEA:InterPro.
DR Gene3D; 3.30.750.24; -; 1.
DR InterPro; IPR011547; SLC26A/SulP_dom.
DR InterPro; IPR001902; SLC26A/SulP_fam.
DR InterPro; IPR030303; SLC26A8.
DR InterPro; IPR002645; STAS_dom.
DR InterPro; IPR036513; STAS_dom_sf.
DR PANTHER; PTHR11814; PTHR11814; 1.
DR PANTHER; PTHR11814:SF11; PTHR11814:SF11; 1.
DR Pfam; PF01740; STAS; 1.
DR Pfam; PF00916; Sulfate_transp; 1.
DR SUPFAM; SSF52091; SSF52091; 1.
DR PROSITE; PS50801; STAS; 1.
PE 2: Evidence at transcript level;
KW Anion exchange; Developmental protein; Differentiation; Glycoprotein;
KW Ion transport; Meiosis; Membrane; Reference proteome; Spermatogenesis;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..727
FT /note="Testis anion transporter 1"
FT /id="PRO_0000322587"
FT TOPO_DOM 1..23
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 24..44
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 45..47
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 69..74
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 96..130
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 152..160
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 182..198
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 199..219
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 220..235
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 257..283
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..304
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 305..360
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 382..383
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 384..404
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 405..424
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 446..727
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT DOMAIN 471..722
FT /note="STAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00198"
FT REGION 592..727
FT /note="Interaction with RACGAP1"
FT /evidence="ECO:0000250|UniProtKB:Q96RN1"
FT CARBOHYD 120
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 727 AA; 80618 MW; 272F87E176CAA9C5 CRC64;
MLTIFPFLEW MCMYRLKDWL LGDLLAGISV GLVQVPQGLT LSLLARQLIP PLNIAYAAFC
SSVIYVIFGS CHQMSIGSFF LVSALLINVL KISPLNNGHL VMGSFLKDEF SAPSYLMGYN
KSLSVVATTT FLTGIIQLIM GVLGLGFIAT YLPESAMSAY LAAVALHIML SQLTCIFGIM
ISFHAGPISF FYDIINYCVA LPKANSTSIL LFLTVVVALR INKCIRISFN QYPIEFPMEL
FLIIGFTVIG NKITMATETS QTLIDMIPYS FLFPVTPDFS VLPKIILQAI SLSLVSSFLL
VFLGKKIASL HNYSVNSNQD LIAIGLCNVV SSFFRSCVFT GAVARTIIQD KSGGRQQFAS
LVGAGVMLLL MVKMGHFFYA LPNAVLAGII LSNVVPYLET ISNLPSLWRQ DQYDCALWMM
TFSSSIFLGL DIGLIISVVS AFFITSVRSH RAKILLLGQI PNTNIYRSVN DYREIITIPG
VKIFQCCSSI TFVNVYYLKH KLLKEVGMVR VPLKEEEIFS LFNSSDTSLQ GEKICRCFCN
CDDLEPLPRI LYTERFENKL DPDASSVNLI HCSHFESVNT SQTASEDQVP YTVSSMSQKN
QGQQYEEVEK VWLPNNSSRN SSPGLPDVAE SQGRRSLIPY SDASLLPSVH TIILDFSMVH
YVDSQGLVVL RQICNAFRNA NILILIAGCH SSLFCTGITL PDHITKCQQL SLNSLSRLPS
FHLQHIF
