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BENY_ASPTE
ID   BENY_ASPTE              Reviewed;        1110 AA.
AC   P9WEU8;
DT   12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT   12-AUG-2020, sequence version 1.
DT   25-MAY-2022, entry version 5.
DE   RecName: Full=Nonribisomal peptide synthetase benY {ECO:0000303|PubMed:28604695};
DE            Short=NRPS benY {ECO:0000303|PubMed:28604695};
DE            EC=6.3.1.- {ECO:0000305|PubMed:28604695};
DE   AltName: Full=Benzomalvin biosynthesis cluster protein Y {ECO:0000303|PubMed:28604695};
GN   Name=benY {ECO:0000303|PubMed:28604695};
OS   Aspergillus terreus.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=33178;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, AND
RP   PATHWAY.
RC   STRAIN=ATCC 20542 / MF4845;
RX   PubMed=28604695; DOI=10.1038/nchembio.2408;
RA   Clevenger K.D., Bok J.W., Ye R., Miley G.P., Verdan M.H., Velk T., Chen C.,
RA   Yang K., Robey M.T., Gao P., Lamprecht M., Thomas P.M., Islam M.N.,
RA   Palmer J.M., Wu C.C., Keller N.P., Kelleher N.L.;
RT   "A scalable platform to identify fungal secondary metabolites and their
RT   gene clusters.";
RL   Nat. Chem. Biol. 13:895-901(2017).
CC   -!- FUNCTION: Nonribisomal peptide synthetase; part of the gene cluster
CC       that mediates the biosynthesis of benzomalvin A and D
CC       (PubMed:28604695). The pathway begins with the loading of amino acid
CC       precursors onto the A domains of the non ribosomal peptide synthetases
CC       benY and benZ (PubMed:28604695). BenY and the A1 domain of benZ are
CC       loaded with anthranilate (Anth), while the A2 domain of benZ is loaded
CC       with phenylalanine (Phe) (PubMed:28604695). N-methylation of Phe by the
CC       methyltransferase benX may happen before loading of Phe onto benZ,
CC       after loading of Phe, or after dipeptide formation (PubMed:28604695).
CC       Condensation of Anth with the secondary amine of NmPhe or Phe is
CC       catalyzed by the C1 domain of benZ, forming a dipeptide intermediate
CC       (PubMed:28604695). This is followed by in trans condensation of the
CC       Anth-NmPhe dipeptide with Anth bound to the T domain of benY by the C2
CC       domain of benZ to form the linear tripeptide Anth-NmPhe-Anth
CC       (PubMed:28604695). Cyclization and release of the tripeptide is then
CC       catalyzed by the C-terminal C domain of benY and the resulting 11-
CC       member macrocyclic intermediate is expected to spontaneously collapse
CC       to form the benzodiazepine core (PubMed:28604695). Benzomalvin A is in
CC       conformational equilibrium with its atropisomer, benzomalvin D
CC       (PubMed:28604695). {ECO:0000269|PubMed:28604695}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:28604695}.
CC   -!- DOMAIN: NRP synthetases are composed of discrete domains (adenylation
CC       (A), thiolation (T) or peptidyl carrier protein (PCP) and condensation
CC       (C) domains) which when grouped together are referred to as a single
CC       module. Each module is responsible for the recognition (via the A
CC       domain) and incorporation of a single amino acid into the growing
CC       peptide product. Thus, an NRP synthetase is generally composed of one
CC       or more modules and can terminate in a thioesterase domain (TE) that
CC       releases the newly synthesized peptide from the enzyme. Occasionally,
CC       epimerase (E) domains (responsible for L- to D-amino acid conversion)
CC       are present within the NRP synthetase. BenY as the following
CC       monomodular architecture: A-T-C. {ECO:0000305|PubMed:28604695}.
CC   -!- DISRUPTION PHENOTYPE: Leads to the accumulation of the benzomalvin
CC       dipeptide precursor anthranilate-N-methylphenylalanine (Anth-NmPhe).
CC       {ECO:0000269|PubMed:28604695}.
CC   -!- SIMILARITY: Belongs to the NRP synthetase family. {ECO:0000305}.
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DR   EMBL; KX449366; AQM58288.1; -; Genomic_DNA.
DR   AlphaFoldDB; P9WEU8; -.
DR   SMR; P9WEU8; -.
DR   VEuPathDB; FungiDB:ATEG_06113; -.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1200.10; -; 1.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.30.559.10; -; 1.
DR   Gene3D; 3.40.50.12780; -; 1.
DR   InterPro; IPR010071; AA_adenyl_domain.
DR   InterPro; IPR036736; ACP-like_sf.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   InterPro; IPR042099; ANL_N_sf.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   InterPro; IPR001242; Condensatn.
DR   InterPro; IPR009081; PP-bd_ACP.
DR   InterPro; IPR006162; Ppantetheine_attach_site.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF00668; Condensation; 1.
DR   Pfam; PF00550; PP-binding; 1.
DR   SUPFAM; SSF47336; SSF47336; 1.
DR   TIGRFAMs; TIGR01733; AA-adenyl-dom; 1.
DR   PROSITE; PS50075; CARRIER; 1.
DR   PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE   3: Inferred from homology;
KW   Ligase; Phosphopantetheine; Phosphoprotein.
FT   CHAIN           1..1110
FT                   /note="Nonribisomal peptide synthetase benY"
FT                   /id="PRO_0000450604"
FT   DOMAIN          575..651
FT                   /note="Carrier"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258,
FT                   ECO:0000305|PubMed:28604695"
FT   REGION          47..443
FT                   /note="Adenylation"
FT                   /evidence="ECO:0000255, ECO:0000305|PubMed:28604695"
FT   REGION          713..1025
FT                   /note="Condensation"
FT                   /evidence="ECO:0000255, ECO:0000305|PubMed:28604695"
FT   MOD_RES         612
FT                   /note="O-(pantetheine 4'-phosphoryl)serine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ   SEQUENCE   1110 AA;  121887 MW;  02AFA3571C035AA2 CRC64;
     MVSRKPALAV KELGCISDRD LRQLQRWNLR AATPATDQLM HEIIHQRALE FPEKIAVEAW
     NGTFTYQQLD RLASHLASCL ASRGIGSNDF VPISFHKSRW AIVAMLAVNK SGAAFVPVDP
     SLPAGRVIHI LRQTEARVAL ACDKRSTAMS EAGISVITVA DSMDCEHLDK PLWSPSFPGH
     NAPAYCLFTS GSTGEPKGCV VGHAAFASIA SHSQSAYIHS GSRVLQFASL GFGMGLFEVF
     CTLSTGATLC IPSDEDRMNC LAHAMTSMNV TWTILSPTTL STLSPADMDC LVTVVTGGEP
     LSESQVTVWA PHVRLLQLYG LTECSGMFTV SDQIFSSDNP ERNIGYPISG RCWIADPQDH
     HRLRAIGAVG ELLIDTPNLA QNYLHNPAKT AAAFISPPGW IEDQLPARQS RPAVLYKTGD
     LARFNLDGSI CHLGRKDHQL KVRGQRVEPG ELEHHLRQLF PAVGDVVVDM ACPVEANGVA
     SLTAFILQEN ECSDDLFVEP TEAFLECVQS VRQSLTKIVP AYMIPNLFLR LGTMPKTVSG
     KKDRRRLRQE VGLLTWDRLR KYMTVDNARG RAAHTLETES EKILAQIWAD LLHLDVGTLG
     PEDDILALGA DSITAMRAVA MARIRGLGLT VSDIFATPTL AEMAQSARVV PTTAVTTHRS
     VSLVDDNVRE LCLSHLREQT SLLDSDEQTP LILPATGMQK FFLDRSSFDY FAYILDGDVD
     FDRMQAACTT AVNQHSILRT VFVQNASGIF QVTLSSIPTA LYHITTARNV ADVSEKLWSP
     NTSKTVTLDH PATRFMLVSN PDAQQHALIL RLSHSQYDGL SWPNLVGAIA ATYNGSALSP
     CLQFSDYIRC RYQQDTTAGY NFWRRYLLGY TPTHMRDCTF EATTQSKAVS TDSDAVNVHH
     TIPSPPDTPD GITMATLIKA AGALVLGQLT RRPDIVIGQT VHGRSSLPLA GIETILGPCL
     NFVPIHVQIH PTWTAERFLH HVQDSHIQTT AYDYLELADI IEQSTSWAPG TSLGAIFHHQ
     NIDTKMEISL QGMKNSKTIH HLTGSYIHQQ MRSEVWVYSM PVDQGLEISI RGSSHVISAP
     QADELARKLG AFVQTLARHP EQALVNIMAP
 
 
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