S28A1_MOUSE
ID S28A1_MOUSE Reviewed; 648 AA.
AC E9PXX9; Q6P8I9;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Sodium/nucleoside cotransporter 1;
DE AltName: Full=Concentrative nucleoside transporter 1;
DE Short=CNT 1;
DE AltName: Full=Na(+)/nucleoside cotransporter 1;
DE AltName: Full=Sodium-coupled nucleoside transporter 1;
DE AltName: Full=Solute carrier family 28 member 1;
GN Name=Slc28a1 {ECO:0000312|MGI:MGI:3605073};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Sodium-dependent and pyrimidine-selective transporter.
CC Exhibits the transport characteristics of the nucleoside transport
CC system cit or N2 subtype (N2/cit) (selective for pyrimidine nucleosides
CC and adenosine). Transports uridine, cytidine, thymidine, and
CC nucleoside-derived drugs. It has the following selective inhibition:
CC adenosine, thymidine, cytidine, uridine >> guanosine, inosine.
CC {ECO:0000250|UniProtKB:O00337}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Na(+)(out) + uridine(out) = Na(+)(in) + uridine(in);
CC Xref=Rhea:RHEA:69887, ChEBI:CHEBI:16704, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000250|UniProtKB:O00337};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Na(+)(out) + thymidine(out) = Na(+)(in) + thymidine(in);
CC Xref=Rhea:RHEA:69891, ChEBI:CHEBI:17748, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000250|UniProtKB:O00337};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cytidine(out) + Na(+)(out) = cytidine(in) + Na(+)(in);
CC Xref=Rhea:RHEA:69895, ChEBI:CHEBI:17562, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000250|UniProtKB:O00337};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:O00337};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the concentrative nucleoside transporter (CNT)
CC (TC 2.A.41) family. {ECO:0000305}.
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DR EMBL; BC061230; AAH61230.1; -; mRNA.
DR RefSeq; NP_001004184.2; NM_001004184.3.
DR RefSeq; XP_006541039.1; XM_006540976.3.
DR RefSeq; XP_006541040.1; XM_006540977.3.
DR RefSeq; XP_006541041.1; XM_006540978.3.
DR RefSeq; XP_006541042.1; XM_006540979.3.
DR AlphaFoldDB; E9PXX9; -.
DR SMR; E9PXX9; -.
DR STRING; 10090.ENSMUSP00000026820; -.
DR iPTMnet; Q6P8I9; -.
DR PhosphoSitePlus; E9PXX9; -.
DR PaxDb; E9PXX9; -.
DR PRIDE; E9PXX9; -.
DR ProteomicsDB; 339181; -.
DR Antibodypedia; 65918; 71 antibodies from 19 providers.
DR DNASU; 434203; -.
DR Ensembl; ENSMUST00000026820; ENSMUSP00000026820; ENSMUSG00000025726.
DR Ensembl; ENSMUST00000119083; ENSMUSP00000112421; ENSMUSG00000025726.
DR GeneID; 434203; -.
DR KEGG; mmu:434203; -.
DR UCSC; uc009ibs.1; mouse.
DR CTD; 9154; -.
DR MGI; MGI:3605073; Slc28a1.
DR VEuPathDB; HostDB:ENSMUSG00000025726; -.
DR eggNOG; KOG3747; Eukaryota.
DR GeneTree; ENSGT00390000016025; -.
DR HOGENOM; CLU_016813_3_2_1; -.
DR InParanoid; E9PXX9; -.
DR OMA; IVWHTVI; -.
DR OrthoDB; 471043at2759; -.
DR PhylomeDB; E9PXX9; -.
DR TreeFam; TF314131; -.
DR Reactome; R-MMU-83936; Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.
DR BioGRID-ORCS; 434203; 3 hits in 71 CRISPR screens.
DR ChiTaRS; Slc28a1; mouse.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; E9PXX9; protein.
DR Bgee; ENSMUSG00000025726; Expressed in proximal tubule and 26 other tissues.
DR GO; GO:0016324; C:apical plasma membrane; ISO:MGI.
DR GO; GO:0031526; C:brush border membrane; IDA:ARUK-UCL.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; ISO:MGI.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:1901474; F:azole transmembrane transporter activity; ISO:MGI.
DR GO; GO:0015212; F:cytidine transmembrane transporter activity; ISO:MGI.
DR GO; GO:0005337; F:nucleoside transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005415; F:nucleoside:sodium symporter activity; IBA:GO_Central.
DR GO; GO:0005350; F:pyrimidine nucleobase transmembrane transporter activity; ISO:MGI.
DR GO; GO:0015389; F:pyrimidine- and adenine-specific:sodium symporter activity; ISO:MGI.
DR GO; GO:0015293; F:symporter activity; IBA:GO_Central.
DR GO; GO:0015213; F:uridine transmembrane transporter activity; ISO:MGI.
DR GO; GO:0045117; P:azole transmembrane transport; ISO:MGI.
DR GO; GO:0015861; P:cytidine transport; ISO:MGI.
DR GO; GO:1901642; P:nucleoside transmembrane transport; ISO:MGI.
DR GO; GO:0015855; P:pyrimidine nucleobase transport; ISO:MGI.
DR GO; GO:0072531; P:pyrimidine-containing compound transmembrane transport; ISO:MGI.
DR GO; GO:0015862; P:uridine transport; ISO:MGI.
DR InterPro; IPR008276; C_nuclsd_transpt.
DR InterPro; IPR018270; C_nuclsd_transpt_met_bac.
DR InterPro; IPR030212; CNT1/CNT2.
DR InterPro; IPR011657; CNT_C_dom.
DR InterPro; IPR002668; CNT_N_dom.
DR InterPro; IPR011642; Gate_dom.
DR PANTHER; PTHR10590; PTHR10590; 1.
DR PANTHER; PTHR10590:SF16; PTHR10590:SF16; 1.
DR Pfam; PF07670; Gate; 1.
DR Pfam; PF07662; Nucleos_tra2_C; 1.
DR Pfam; PF01773; Nucleos_tra2_N; 1.
DR TIGRFAMs; TIGR00804; nupC; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..648
FT /note="Sodium/nucleoside cotransporter 1"
FT /id="PRO_0000455292"
FT TOPO_DOM 1..83
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 84..104
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 105..108
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 130..147
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 169..175
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..201
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 223..265
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 287..294
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 295..318
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 319..339
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 340..360
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 361
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 362..380
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 381..427
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 428..448
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 449..470
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 471..491
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 492..531
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 532..552
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 553..571
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 572..592
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 593..648
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT CARBOHYD 605
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 625
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 643
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 96
FT /note="G -> S (in Ref. 2; AAH61230)"
FT /evidence="ECO:0000305"
FT CONFLICT 137
FT /note="K -> E (in Ref. 2; AAH61230)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 648 AA; 71174 MW; C32DCEA9133147E1 CRC64;
MADDTPRQRE SISLTPVAHG LENMGAEFLE IMEEGQLPHR HSSLPEGGGS RSKAVWKPFS
RWRSLQPTVQ ARSLCREHWQ LFEWISKGLL STAYIGFLIV ACLLDFPRAL ALFVITCVVL
VFLAYNLLKR LLGSKLKKCV KFQGHSCLSL WLKRGLALAA GLGVILWLSL DTAQRPEQLV
SFAGICVFLV LLFAGSKHHR AVSWRAVSWG LGLQFVLGLF VIRTEPGFVA FQWLGDQIRV
FLSYTEAGSS FVFGEALVKD VFAFQVLPII VFFSCVMSVL YYLGLMQWVI LKIAWLMQVT
MGTSATETLS VAGNIFVSQT EAPLLIRPYL ADMTLSEVHV VMTGGYATIA GSLLGAYISF
GIDASSLIAA SVMAAPCALA LSKLVYPEVE ESKFRSEEGV KLTYGDAQNL VEAASAGAAI
SVKVVANIAA NLIAFLAVLA FINAALSWLG DMVDIQGLSF QLICSYVLRP VAFLMGVAWE
DCPVVAELLG IKLFLNEFVA YQELSQYKQR RLAGAEEWLG DKKQWISVRA EILTTYALCG
FANFSSIGIM LGGLTSMVPQ RRSDFSQIVL RALITGAFVS LVNACVAGIL YVPRGVEVDC
MSLLNQTVSS SSFEVYLCCR QVFQNTSLEF GQEALHNCCR FYNHTVCT
