S29A1_RAT
ID S29A1_RAT Reviewed; 457 AA.
AC O54698;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Equilibrative nucleoside transporter 1;
DE AltName: Full=Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter;
DE Short=Equilibrative NBMPR-sensitive nucleoside transporter;
DE AltName: Full=Nucleoside transporter, es-type;
DE AltName: Full=Solute carrier family 29 member 1;
GN Name=Slc29a1; Synonyms=Ent1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=Sprague-Dawley; TISSUE=Jejunum;
RX PubMed=9353301; DOI=10.1074/jbc.272.45.28423;
RA Yao S.Y.M., Ng A.M.L., Muzyka W.R., Griffiths M., Cass C.E., Baldwin S.A.,
RA Young J.D.;
RT "Molecular cloning and functional characterization of
RT nitrobenzylthioinosine (NBMPR)-sensitive (es) and NBMPR-insensitive (ei)
RT equilibrative nucleoside transporter proteins (rENT1 and rENT2) from rat
RT tissues.";
RL J. Biol. Chem. 272:28423-28430(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Mediates both influx and efflux of nucleosides across the
CC membrane (equilibrative transporter). It is sensitive (ES) to low
CC concentrations of the inhibitor nitrobenzylmercaptopurine riboside
CC (NBMPR) and is sodium-independent. It has a higher affinity for
CC adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer
CC chemotherapeutics drugs). {ECO:0000269|PubMed:9353301}.
CC -!- SUBUNIT: Identified in a complex with STOM. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Basolateral cell membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}. Apical cell membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Cell membrane
CC {ECO:0000269|PubMed:9353301}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:9353301}. Note=Predominantly localized in the
CC basolateral membrane in polarized MDCK cells. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in jejunum, liver and lung.
CC -!- PTM: Glycosylated.
CC -!- SIMILARITY: Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.
CC {ECO:0000305}.
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DR EMBL; AF015304; AAB88049.1; -; mRNA.
DR EMBL; BC078789; AAH78789.1; -; mRNA.
DR RefSeq; NP_113872.1; NM_031684.2.
DR RefSeq; XP_006244622.1; XM_006244560.2.
DR RefSeq; XP_006244623.1; XM_006244561.2.
DR RefSeq; XP_006244624.1; XM_006244562.3.
DR RefSeq; XP_006244625.1; XM_006244563.3.
DR RefSeq; XP_017452108.1; XM_017596619.1.
DR AlphaFoldDB; O54698; -.
DR SMR; O54698; -.
DR STRING; 10116.ENSRNOP00000026831; -.
DR BindingDB; O54698; -.
DR ChEMBL; CHEMBL4604; -.
DR DrugCentral; O54698; -.
DR TCDB; 2.A.57.1.3; the equilibrative nucleoside transporter (ent) family.
DR GlyGen; O54698; 3 sites.
DR iPTMnet; O54698; -.
DR PhosphoSitePlus; O54698; -.
DR jPOST; O54698; -.
DR PaxDb; O54698; -.
DR Ensembl; ENSRNOT00000108087; ENSRNOP00000082743; ENSRNOG00000019752.
DR GeneID; 63997; -.
DR KEGG; rno:63997; -.
DR UCSC; RGD:61899; rat.
DR CTD; 2030; -.
DR RGD; 61899; Slc29a1.
DR eggNOG; KOG1479; Eukaryota.
DR GeneTree; ENSGT00950000182898; -.
DR HOGENOM; CLU_021611_6_0_1; -.
DR InParanoid; O54698; -.
DR OMA; KIMFINS; -.
DR OrthoDB; 559763at2759; -.
DR PhylomeDB; O54698; -.
DR Reactome; R-RNO-83936; Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.
DR Reactome; R-RNO-9748787; Azathioprine ADME.
DR PRO; PR:O54698; -.
DR Proteomes; UP000002494; Chromosome 9.
DR Bgee; ENSRNOG00000019752; Expressed in lung and 19 other tissues.
DR Genevisible; O54698; RN.
DR GO; GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
DR GO; GO:0016323; C:basolateral plasma membrane; ISS:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IDA:RGD.
DR GO; GO:0098794; C:postsynapse; IDA:SynGO.
DR GO; GO:0098793; C:presynapse; IDA:SynGO.
DR GO; GO:0080122; F:AMP transmembrane transporter activity; ISO:RGD.
DR GO; GO:0005326; F:neurotransmitter transmembrane transporter activity; ISO:RGD.
DR GO; GO:0005337; F:nucleoside transmembrane transporter activity; IDA:RGD.
DR GO; GO:0015211; F:purine nucleoside transmembrane transporter activity; ISO:RGD.
DR GO; GO:0015213; F:uridine transmembrane transporter activity; ISO:RGD.
DR GO; GO:0032238; P:adenosine transport; ISO:RGD.
DR GO; GO:0071333; P:cellular response to glucose stimulus; IEP:RGD.
DR GO; GO:0071456; P:cellular response to hypoxia; IEP:RGD.
DR GO; GO:0060079; P:excitatory postsynaptic potential; IMP:RGD.
DR GO; GO:0007595; P:lactation; IEP:RGD.
DR GO; GO:0098810; P:neurotransmitter reuptake; ISO:RGD.
DR GO; GO:0006836; P:neurotransmitter transport; ISO:RGD.
DR GO; GO:0001504; P:neurotransmitter uptake; ISO:RGD.
DR GO; GO:1901642; P:nucleoside transmembrane transport; ISO:RGD.
DR GO; GO:0015858; P:nucleoside transport; IDA:RGD.
DR GO; GO:0015860; P:purine nucleoside transmembrane transport; ISO:RGD.
DR GO; GO:0072531; P:pyrimidine-containing compound transmembrane transport; ISO:RGD.
DR GO; GO:0030431; P:sleep; IMP:RGD.
DR GO; GO:0015862; P:uridine transport; IMP:RGD.
DR InterPro; IPR030195; ENT1.
DR InterPro; IPR034764; ENT1/ENT2.
DR InterPro; IPR002259; Eqnu_transpt.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR10332; PTHR10332; 1.
DR PANTHER; PTHR10332:SF9; PTHR10332:SF9; 1.
DR Pfam; PF01733; Nucleoside_tran; 1.
DR PIRSF; PIRSF016379; ENT; 1.
DR PRINTS; PR01130; DERENTRNSPRT.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00939; 2a57; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..457
FT /note="Equilibrative nucleoside transporter 1"
FT /id="PRO_0000209339"
FT TOPO_DOM 1..12
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 13..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..82
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..111
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 131..138
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 158..174
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..206
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 228..291
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..311
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 312..323
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 324..343
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 344..360
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..379
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 380..394
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..414
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 415..432
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 433..453
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 454..457
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 255..275
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 254
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 273
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q99808"
FT CARBOHYD 44
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 48
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 54
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 457 AA; 50017 MW; A9DDF4F0D7F8D70A CRC64;
MTTSHQPQDR YKAVWLIFFV LGLGTLLPWN FFITATQYFT SRLNTSQNIS LVTNQSCEST
EALADPSVSL PARSSLSAIF NNVMTLCAML PLLIFTCLNS FLHQKVSQSL RILGSLLAIL
LVFLVTATLV KVQMDALSFF IITMIKIVLI NSFGAILQAS LFGLAGVLPA NYTAPIMSGQ
GLAGFFTSVA MICAVASGSK LSESAFGYFI TACAVVILAI LCYLALPWME FYRHYLQLNL
AGPAEQETKL DLISEGEEPR GGREESGVPG PNSLPANRNQ SIKAILKSIW VLALSVCFIF
TVTIGLFPAV TAEVESSIAG TSPWKNCYFI PVACFLNFNV FDWLGRSLTA ICMWPGQDSR
WLPVLVACRV VFIPLLMLCN VKQHHYLPSL FKHDVWFITF MAAFAFSNGY LASLCMCFGP
KKVKPAEAET AGNIMSFFLC LGLALGAVLS FLLRALV
