S29A2_MOUSE
ID S29A2_MOUSE Reviewed; 456 AA.
AC Q61672; Q9JIT8;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 08-MAR-2011, sequence version 2.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Equilibrative nucleoside transporter 2;
DE AltName: Full=36 kDa hydrophobic nucleolar protein;
DE AltName: Full=36 kDa nucleolar protein HNP36;
DE AltName: Full=Delayed-early response protein 12;
DE AltName: Full=Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter;
DE Short=Equilibrative NBMPR-insensitive nucleoside transporter;
DE AltName: Full=Nucleoside transporter, ei-type;
DE AltName: Full=Solute carrier family 29 member 2;
GN Name=Slc29a2; Synonyms=Der12, Ent2, Hnp36;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=CD-1; TISSUE=Brain;
RX PubMed=11085929; DOI=10.1042/bj3520363;
RA Kiss A., Farah K., Kim J., Garriock R.J., Drysdale T.A., Hammond J.R.;
RT "Molecular cloning and functional characterization of inhibitor-sensitive
RT (mENT1) and inhibitor-resistant (mENT2) equilibrative nucleoside
RT transporters from mouse brain.";
RL Biochem. J. 352:363-372(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 64-456.
RC STRAIN=BALB/cJ; TISSUE=Fibroblast;
RX PubMed=7639753; DOI=10.1006/bbrc.1995.2133;
RA Williams J.B., Lanahan A.A.;
RT "A mammalian delayed-early response gene encodes HNP36, a novel, conserved
RT nucleolar protein.";
RL Biochem. Biophys. Res. Commun. 213:325-333(1995).
CC -!- FUNCTION: Mediates equilibrative transport of purine and pyrimidine
CC nucleosides, and the purine base hypoxanthine.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.75 mM for adenosine;
CC -!- SUBCELLULAR LOCATION: Nucleus membrane; Multi-pass membrane protein.
CC Basolateral cell membrane {ECO:0000250}; Multi-pass membrane protein
CC {ECO:0000250}.
CC -!- INDUCTION: By platelet derived growth factor (PDGF) and fibroblast
CC growth factor (FGF).
CC -!- SIMILARITY: Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA60381.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF183397; AAF78477.1; -; mRNA.
DR EMBL; CH466612; EDL33088.1; -; Genomic_DNA.
DR EMBL; BC048958; AAH48958.1; -; mRNA.
DR EMBL; X86682; CAA60381.1; ALT_INIT; mRNA.
DR CCDS; CCDS50354.1; -.
DR PIR; JC4195; JC4195.
DR RefSeq; NP_031880.2; NM_007854.3.
DR AlphaFoldDB; Q61672; -.
DR SMR; Q61672; -.
DR STRING; 10090.ENSMUSP00000025826; -.
DR ChEMBL; CHEMBL1287612; -.
DR TCDB; 2.A.57.1.2; the equilibrative nucleoside transporter (ent) family.
DR GlyGen; Q61672; 1 site.
DR iPTMnet; Q61672; -.
DR PhosphoSitePlus; Q61672; -.
DR EPD; Q61672; -.
DR MaxQB; Q61672; -.
DR PaxDb; Q61672; -.
DR PeptideAtlas; Q61672; -.
DR PRIDE; Q61672; -.
DR ProteomicsDB; 253379; -.
DR Antibodypedia; 16257; 142 antibodies from 23 providers.
DR DNASU; 13340; -.
DR Ensembl; ENSMUST00000236152; ENSMUSP00000157780; ENSMUSG00000024891.
DR GeneID; 13340; -.
DR KEGG; mmu:13340; -.
DR UCSC; uc008gbv.2; mouse.
DR CTD; 3177; -.
DR MGI; MGI:1345278; Slc29a2.
DR VEuPathDB; HostDB:ENSMUSG00000024891; -.
DR eggNOG; KOG1479; Eukaryota.
DR GeneTree; ENSGT00950000182898; -.
DR HOGENOM; CLU_021611_6_0_1; -.
DR InParanoid; Q61672; -.
DR OMA; FNIMDWV; -.
DR OrthoDB; 559763at2759; -.
DR PhylomeDB; Q61672; -.
DR TreeFam; TF313950; -.
DR Reactome; R-MMU-83936; Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.
DR Reactome; R-MMU-9748787; Azathioprine ADME.
DR BioGRID-ORCS; 13340; 1 hit in 76 CRISPR screens.
DR PRO; PR:Q61672; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q61672; protein.
DR Bgee; ENSMUSG00000024891; Expressed in ectoplacental cone and 91 other tissues.
DR ExpressionAtlas; Q61672; baseline and differential.
DR Genevisible; Q61672; MM.
DR GO; GO:0016323; C:basolateral plasma membrane; ISS:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IGI:MGI.
DR GO; GO:0098793; C:presynapse; IEA:GOC.
DR GO; GO:0005326; F:neurotransmitter transmembrane transporter activity; IGI:ARUK-UCL.
DR GO; GO:0005337; F:nucleoside transmembrane transporter activity; IDA:MGI.
DR GO; GO:0015211; F:purine nucleoside transmembrane transporter activity; IGI:ARUK-UCL.
DR GO; GO:0015213; F:uridine transmembrane transporter activity; ISO:MGI.
DR GO; GO:0015853; P:adenine transport; ISO:MGI.
DR GO; GO:0032238; P:adenosine transport; IGI:ARUK-UCL.
DR GO; GO:0032869; P:cellular response to insulin stimulus; IEA:Ensembl.
DR GO; GO:0015854; P:guanine transport; ISO:MGI.
DR GO; GO:0035344; P:hypoxanthine transport; ISO:MGI.
DR GO; GO:0007595; P:lactation; IEA:Ensembl.
DR GO; GO:0098810; P:neurotransmitter reuptake; ISO:MGI.
DR GO; GO:0006836; P:neurotransmitter transport; ISO:MGI.
DR GO; GO:0001504; P:neurotransmitter uptake; IGI:ARUK-UCL.
DR GO; GO:1901642; P:nucleoside transmembrane transport; ISO:MGI.
DR GO; GO:0015858; P:nucleoside transport; IDA:MGI.
DR GO; GO:0015860; P:purine nucleoside transmembrane transport; IGI:ARUK-UCL.
DR GO; GO:0072531; P:pyrimidine-containing compound transmembrane transport; ISO:MGI.
DR GO; GO:0035364; P:thymine transport; ISO:MGI.
DR GO; GO:0015862; P:uridine transport; ISO:MGI.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR034764; ENT1/ENT2.
DR InterPro; IPR030197; ENT2.
DR InterPro; IPR002259; Eqnu_transpt.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR10332; PTHR10332; 1.
DR PANTHER; PTHR10332:SF8; PTHR10332:SF8; 1.
DR Pfam; PF01733; Nucleoside_tran; 1.
DR PIRSF; PIRSF016379; ENT; 1.
DR PRINTS; PR01130; DERENTRNSPRT.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00939; 2a57; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Membrane; Nucleus; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..456
FT /note="Equilibrative nucleoside transporter 2"
FT /id="PRO_0000209341"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 192..212
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 323..343
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 360..380
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 396..416
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 432..452
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 248..277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 252
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q14542"
FT CARBOHYD 56
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 456 AA; 50255 MW; A9FE7CA037A4468F CRC64;
MARGNAPRDS YHLVGISFFI LGLGTLLPWN FFITAIPYFQ GRLAGTNSSA ETMGTNHTSP
TDTFNFNNWV TLLSQLPLLL FTLLNSFLYQ CIPESVRILG SLLAILLLFA LTAALVKVDL
SPGLFFSVTM ASVWFINSFC AVLQGSLFGQ LGTMPSTYST LFLSGQGLAG IFAALAMLMS
LASGVDAQTS ALGYFITPCV GILLSIVCYL SLPHLKFARY YLTEKLSQAP TQELETKAEL
LQADEKNGVP ISPQQASPTL DLDPEKEPEP EEPQKPGKPS VFVVFRKIWL TALCLVLVFT
VTLSVFPAIT AMVTTSSNSP GKWGLFFNPI CCFLLFNVMD WLGRSLTSYF LWPDEDSQQL
LPLLVCLRFL FVPLFMLCHV PQHARLPIIF RQDAYFITFM LLFAVSNGYL VSLTMCLAPR
QVLPHEREVA GALMTFFLAL GLSCGASLSF LFKALL
