S29A4_DANRE
ID S29A4_DANRE Reviewed; 518 AA.
AC A1L272;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Equilibrative nucleoside transporter 4;
DE AltName: Full=Solute carrier family 29 member 4;
GN Name=slc29a4; ORFNames=zgc:158679;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Functions as a polyspecific organic cation transporter,
CC efficiently transporting many organic cations such as monoamine
CC neurotransmitters 1-methyl-4-phenylpyridinium and biogenic amines
CC including serotonin, dopamine, norepinephrine and epinephrine. May play
CC a role in regulating central nervous system homeostasis of monoamine
CC neurotransmitters. May be involved in luminal transport of organic
CC cations in the kidney and seems to use luminal proton gradient to drive
CC organic cation reabsorption. Does not seem to transport nucleoside and
CC nucleoside analogs such as uridine, cytidine, thymidine, adenosine,
CC inosine, guanosine, and azidothymidine (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.
CC {ECO:0000305}.
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DR EMBL; BC129376; AAI29377.1; -; mRNA.
DR RefSeq; NP_001074041.1; NM_001080572.1.
DR AlphaFoldDB; A1L272; -.
DR SMR; A1L272; -.
DR STRING; 7955.ENSDARP00000117850; -.
DR PaxDb; A1L272; -.
DR GeneID; 559668; -.
DR KEGG; dre:559668; -.
DR CTD; 559668; -.
DR ZFIN; ZDB-GENE-070112-1932; slc29a4a.
DR eggNOG; KOG1479; Eukaryota.
DR InParanoid; A1L272; -.
DR OrthoDB; 674800at2759; -.
DR PhylomeDB; A1L272; -.
DR Reactome; R-DRE-83936; Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.
DR PRO; PR:A1L272; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0008504; F:monoamine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005337; F:nucleoside transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0065008; P:regulation of biological quality; IEA:UniProt.
DR InterPro; IPR030198; ENT4.
DR InterPro; IPR002259; Eqnu_transpt.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR10332; PTHR10332; 1.
DR PANTHER; PTHR10332:SF4; PTHR10332:SF4; 1.
DR Pfam; PF01733; Nucleoside_tran; 1.
DR PIRSF; PIRSF016379; ENT; 1.
DR PRINTS; PR01130; DERENTRNSPRT.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 2: Evidence at transcript level;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..518
FT /note="Equilibrative nucleoside transporter 4"
FT /id="PRO_0000326253"
FT TOPO_DOM 1..66
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 88..99
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..133
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..157
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 179..198
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 199..218
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 219..229
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 251..342
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 364..376
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 398..407
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 408..428
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 429..439
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 440..460
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 461..476
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 477..497
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 498..518
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 518 AA; 57779 MW; 8D0AA3532572350F CRC64;
MGSKGAERRK QATPGQTPEG NVVMSFSFES YQLEEEDCQG KDISDKGVLA LSEPAFEEAV
PDDRYHGIYF AMLLAGVGFL LPYNSFITDV DYLHHKFEGT SIVFDMGLTY ILVALVAVIL
NNVLVEMLSL HTRITVGYLF ALGPLLFVTI FDVWLERFTI KQAYVINLMS MGTVAFGCTV
QQSSFYGYMG MLPKRYTQGV MTGESTAGVI ISLSRIFTKL LIKDERKNTI IFFVISICMV
LVCFILHLLV RRTRFVQYYT SLARRGLSHA KDHSQHASQY QVHHDVITEE GNGAVGCSPA
GDGCADFAGG NTYVRFDVPK PKMKRSWPGV KDMILHRYVV ARVIWTYMLS IAVTYFITLC
LFPGLESEIK NATLGEWLPI LIMAIFNISD FVGKILAAVP YEWNGTRLLF FSCVRVVFIP
LFIMCVYPAQ MPMFSHPAWP CIFSLFMGIT NGYFGSVPMI HAAGKVAPEQ RELAGNIMTV
SYMSGLMLGS VVAYAAYSFT ASGSSFHSQT GYNFTQGY
